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. 1966 Dec;101(3):680–686.

Enzymic hydrolysis of sphingolipids

Hydrolysis of ceramide lactoside by an enzyme from rat brain

Shimon Gatt 1, Maurice M Rapport 1
PMCID: PMC1270172  PMID: 16742444

Abstract

Ceramide lactoside [1-O-(galactosido-4-β-glucosido)-2-N-acyl-sphingosine] was hydrolysed to ceramide glucoside and galactose by β-galactosidase of rat brain. The reaction was not reversible, required cholate or taurocholate, had optimum pH5·0 and Km 2·2×10−5m. It was inhibited by γ-galactonolactone and galactose as well as by ceramide, sphingosine and fatty acid. Ceramide lactoside could be degraded to ceramide, galactose and glucose by mixtures of rat-brain β-galactosidase and ox-brain β-glucosidase.

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Selected References

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  1. Barnholz Y., Roitman A., Gatt S. Enzymatic hydrolysis of sphingolipids. II. Hydrolysis of sphingomyelin by an enzyme from rat brain. J Biol Chem. 1966 Aug 25;241(16):3731–3737. [PubMed] [Google Scholar]
  2. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  3. GATT S., BERMAN E. R. Studies on brain lipids in Tay-Sachs' disease. I. Isolation of two sialic acid-free glycolipids. J Neurochem. 1963 Jan;10:43–49. doi: 10.1111/j.1471-4159.1963.tb05036.x. [DOI] [PubMed] [Google Scholar]
  4. GATT S., BERMAN E. R. Studies on brain lipids in Tay-Sachs' disease. III. Solubility properties of gangliosides. J Neurochem. 1963 Feb;10:65–72. [PubMed] [Google Scholar]
  5. Gatt S. Enzymatic hydrolysis of sphingolipids. I. Hydrolysis and synthesis of ceramides by an enzyme from rat brain. J Biol Chem. 1966 Aug 25;241(16):3724–3730. [PubMed] [Google Scholar]
  6. Gatt S. Enzymic hydrolysis of sphingolipids: Hydrolysis of ceramide glucoside by an enzyme from ox brain. Biochem J. 1966 Dec;101(3):687–691. [PMC free article] [PubMed] [Google Scholar]
  7. Gatt S., Rapport M. M. Isolation of beta-galactosidase and beta-glucosidase from brain. Biochim Biophys Acta. 1966 Mar 7;113(3):567–576. doi: 10.1016/s0926-6593(66)80014-0. [DOI] [PubMed] [Google Scholar]
  8. Hajra A. K., Bowen D. M., Kishimoto Y., Radin N. S. Cerebroside galactosidase of brain. J Lipid Res. 1966 May;7(3):379–386. [PubMed] [Google Scholar]
  9. KLENK E., DEBUCH H. The lipides. Annu Rev Biochem. 1959;28:39–68. doi: 10.1146/annurev.bi.28.070159.000351. [DOI] [PubMed] [Google Scholar]
  10. KLENK E., LIEDTKE U., GIELEN W. DAS GANGLIOSID DES GEHIRNS BEI DER INFANTILEN AMAUROTISCHEN IDIOTIE VOM TYP TAY-SACHS. Hoppe Seylers Z Physiol Chem. 1963;334:186–192. doi: 10.1515/bchm2.1963.334.1.186. [DOI] [PubMed] [Google Scholar]
  11. Papavassiliou J. Hydrolysis of ceramide lactoside and ceramide glucoside by glycosidases from brain. Isr J Med Sci. 1965 Jul;1(4):624–627. [PubMed] [Google Scholar]
  12. RADIN N. S. Glycolipide determination. Methods Biochem Anal. 1958;6:163–189. doi: 10.1002/9780470110225.ch7. [DOI] [PubMed] [Google Scholar]
  13. RAPPORT M. M., GRAF L., ALONZO N. F. Immunochemical studies of organ and tumor lipids: VIII. Comparison of human tumor and ox spleen cytosides. J Lipid Res. 1960 Jul;1:301–304. [PubMed] [Google Scholar]
  14. RAPPORT M. M., GRAF L., SKIPSKI V. P., ALONZO N. F. Immunochemical studies of organ and tumor lipids. VI. Isolation and properties of cytolipin H. Cancer. 1959 May-Jun;12(3):438–445. doi: 10.1002/1097-0142(195905/06)12:3<438::aid-cncr2820120303>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  15. RAPPORT M. M., SCHNEIDER H., GRAF L. Immunochemical studies of organ and tumor lipids. XI. A simplified preparation of cytolipin H. J Biol Chem. 1962 Apr;237:1056–1059. [PubMed] [Google Scholar]
  16. ROTH H., SEGAL S., BERTOLI D. THE QUANTITATIVE DETERMINATION OF GALACTOSE--AN ENZYMIC METHOD USING GALACTOSE OXIDASE, WITH APPLICATIONS TO BLOOD AND OTHER BIOLOGICAL FLUIDS. Anal Biochem. 1965 Jan;10:32–52. doi: 10.1016/0003-2697(65)90238-1. [DOI] [PubMed] [Google Scholar]
  17. SAMBASIVARAO K., MCCLUER R. H. THIN-LAYER CHROMATOGRAPHIC SEPARATION OF SPHINGOSINE AND RELATED BASES. J Lipid Res. 1963 Jan;4:106–108. [PubMed] [Google Scholar]
  18. SKIPSKI V. P., ARFIN S. M., RAPPORT M. M. Paper chromatography, unsaturated and hydroxy fatty acids. Arch Biochem Biophys. 1960 Apr;87:259–265. doi: 10.1016/0003-9861(60)90170-3. [DOI] [PubMed] [Google Scholar]
  19. SVENNERHOLM E., SVENNERHOLM L. THE SEPARATION OF NEUTRAL BLOOD-SERUM GLYCOLIPIDS BY THIN-LAYER CHROMATOGRAPHY. Biochim Biophys Acta. 1963 Aug 27;70:432–441. doi: 10.1016/0006-3002(63)90773-x. [DOI] [PubMed] [Google Scholar]
  20. SVENNERHOLM L. THE GANGLIOSIDES. J Lipid Res. 1964 Apr;5:145–155. [PubMed] [Google Scholar]
  21. SVENNERHOLM L. The chemical structure of normal human brain and Tay-Sachs gangliosides. Biochem Biophys Res Commun. 1962 Nov 27;9:436–441. doi: 10.1016/0006-291x(62)90030-x. [DOI] [PubMed] [Google Scholar]
  22. WIEGANDT H., BASCHANG G. DIE GEWINNUNG DES ZUCKERANTEILES DER GLYKOSPHINGOLIPIDE DURCH OZONOLYSE UND FRAGMENTIERUNG. Z Naturforsch B. 1965 Feb;20:164–166. [PubMed] [Google Scholar]
  23. YAMAKAWA T., YOKOYAMA S., KISO N. Structure of main globoside of human erythrocytes. J Biochem. 1962 Sep;52:228–229. doi: 10.1093/oxfordjournals.jbchem.a127603. [DOI] [PubMed] [Google Scholar]

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