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. 1966 Dec;101(3):680–686.

Enzymic hydrolysis of sphingolipids

Hydrolysis of ceramide lactoside by an enzyme from rat brain

Shimon Gatt 1, Maurice M Rapport 1
PMCID: PMC1270172  PMID: 16742444

Abstract

Ceramide lactoside [1-O-(galactosido-4-β-glucosido)-2-N-acyl-sphingosine] was hydrolysed to ceramide glucoside and galactose by β-galactosidase of rat brain. The reaction was not reversible, required cholate or taurocholate, had optimum pH5·0 and Km 2·2×10−5m. It was inhibited by γ-galactonolactone and galactose as well as by ceramide, sphingosine and fatty acid. Ceramide lactoside could be degraded to ceramide, galactose and glucose by mixtures of rat-brain β-galactosidase and ox-brain β-glucosidase.

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Selected References

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