Abstract
1. The mucopolysaccharide from the glands of Lorenzini of Squalus acanthias has been isolated and purified. 2. The material is a sulphated polysaccharide containing glucosamine, galactosamine, galactose and traces of an uronic acid. 3. This vertebrate polysaccharide is unusual in appearing to have both amino sugars present in the molecule. The amino sugars are N-acetylated. 4. This molecule is one of a group of related mucopolysaccharides which vary in composition with the animal species. The generic name `lorenzan sulphates' is suggested to describe these substances.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
- BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
- Duncombe W. G. The colorimetric micro-determination of long-chain fatty acids. Biochem J. 1963 Jul;88(1):7–10. doi: 10.1042/bj0880007. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- LUDOWIEG J., DORFMAN A. A micromethod for the colorimetric determination of N-acetyl groups in acid mucopolysaccharides. Biochim Biophys Acta. 1960 Feb 26;38:212–218. doi: 10.1016/0006-3002(60)91233-6. [DOI] [PubMed] [Google Scholar]
- MEYER K., LINKER A., DAVIDSON E. A., WEISSMANN B. The mucopolysaccharides of bovine cornea. J Biol Chem. 1953 Dec;205(2):611–616. [PubMed] [Google Scholar]
- STOFFYN P. J., JEANLOZ R. W. Identification of amino sugars by paper chromatography. Arch Biochem Biophys. 1954 Oct;52(2):373–379. doi: 10.1016/0003-9861(54)90137-x. [DOI] [PubMed] [Google Scholar]
- TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. doi: 10.1042/bj0500298. [DOI] [PMC free article] [PubMed] [Google Scholar]