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. 1967 Jun;103(3):714–719. doi: 10.1042/bj1030714

Ubiquinone deficiency in an auxotroph of Escherichia coli requiring 4-hydroxybenzoic acid

R G Wyn Jones 1
PMCID: PMC1270473  PMID: 4292836

Abstract

1. Escherichia coli 156:53D2 synthesized ubiquinone only when the growth medium was supplemented with 4-hydroxybenzoate acid. 2. Little or no vitamin K2 was formed by the mutant under the growth conditions employed, in contrast with wild-type strains. 3. In the mutant ubiquinone deficiency was correlated with low respiration and with low particulate NADH-oxidase and NADH-cytochrome b1-reductase activity. 4. Preincubation of ubiquinone-deficient particles with ubiquinone-30 largely restored the NADH-oxidase and NADH-cytochrome b1-reductase activities. 5. Various NADH–dye-linked reductases which may be associated with NADH dehydrogenase were not affected by the absence of ubiquinone. 6. The succinate-oxidase complex was less affected than the particulate NADH oxidase by ubiquinone deficiency. 7. A pathway for electrons in the NADH-oxidase complex of the auxotroph of E. coli is proposed and its relationship to the pathway in the wild-type strain is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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