Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Jun;103(3):805–810. doi: 10.1042/bj1030805

Electrophoretic properties of ovomucoid

M D Melamed 1,*
PMCID: PMC1270487  PMID: 6049406

Abstract

1. The nature of the electrophoretic heterogeneity of ovomucoid was investigated. Optimum resolution of the fractions on starch-gel electrophoresis occurred over a narrow range of pH and ionic strength. The pattern was not altered in the presence of 8m-urea but the bands were sharper. Ovomucoid–trypsin complex is stable at pH4·6 but dissociated in 6m-urea. 2. The two major fractions of ovomucoid were eluted from the gels. One of these was virtually free of sialic acid and the other, which contained 0·4mole of sialic acid/mole of protein, split into two components on electrophoresis after neuraminidase treatment. It was concluded that these two components, and likewise the two major fractions of ovomucoid, differ by a unit charge/mol. Differences in sialic acid content account for only part of the electrophoretic heterogeneity of ovomucoid.

Full text

PDF
806

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BEELEY J. G., JEVONS F. R. HETEROGENEITY OF OVOMUCOID. Biochim Biophys Acta. 1965 Mar 1;101:133–135. doi: 10.1016/0926-6534(65)90041-2. [DOI] [PubMed] [Google Scholar]
  3. BIER M., TERMINIELLO L., DUKE J. A., GIBBS R. J., NORD F. F. Investigations on proteins and polymers. X. Composition and fractionation of ovomucoid. Arch Biochem Biophys. 1953 Dec;47(2):465–473. doi: 10.1016/0003-9861(53)90483-4. [DOI] [PubMed] [Google Scholar]
  4. BULL H. B., BREESE K., FERGUSON G. L., SWENSON C. A. THE PH OF UREA SOLUTIONS. Arch Biochem Biophys. 1964 Feb;104:297–304. doi: 10.1016/s0003-9861(64)80017-5. [DOI] [PubMed] [Google Scholar]
  5. CHATTERJEE A. K., MONTGOMERY R. The carbohydrate of ovomucoid. Arch Biochem Biophys. 1962 Dec;99:426–432. doi: 10.1016/0003-9861(62)90290-4. [DOI] [PubMed] [Google Scholar]
  6. CLARK A. Eriochrome black T as a protein dye. Nature. 1962 Dec 15;196:1097–1098. doi: 10.1038/1961097a0. [DOI] [PubMed] [Google Scholar]
  7. FEENEY R. E., ABPLANALP H., CLARY J. J., EDWARDS D. L., CLARK J. R. A genetically varying minor protein constituent of chicken egg white. J Biol Chem. 1963 May;238:1732–1736. [PubMed] [Google Scholar]
  8. FEENEY R. E., STEVENS F. C., OSUGA D. T. The specificities of chicken ovomucoid and ovoinhibitor. J Biol Chem. 1963 Apr;238:1415–1418. [PubMed] [Google Scholar]
  9. FINKENSTADT W. R., LASKOWSKI M., Jr PEPTIDE BOND CLEAVAGE ON TRYPSINTRYPSIN INHIBITOR COMPLEX FORMATION. J Biol Chem. 1965 Feb;240:962–963. [PubMed] [Google Scholar]
  10. FREDERICQ E., DEUTSCH H. F. Studies on ovomucoid. J Biol Chem. 1949 Dec;181(2):499–510. [PubMed] [Google Scholar]
  11. GORDON A. H. The catabolic rate of albumin doubly labelled with 131-I and 14-C in the isolated perfused rat liver. Biochem J. 1962 Mar;82:531–540. doi: 10.1042/bj0820531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JEVONS F. R. The heterogeneity of ovomucoid. Biochim Biophys Acta. 1960 Dec 4;45:384–386. doi: 10.1016/0006-3002(60)91465-7. [DOI] [PubMed] [Google Scholar]
  13. Ketterer B. Ovoglycoprotein, a protein of hen's-egg white. Biochem J. 1965 Aug;96(2):372–376. doi: 10.1042/bj0960372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LLOYD H. M., MEARES J. D. AN ELECTROPHORETIC METHOD FOR RECOVERY OF PROTEINS FROM STARCH GEL. Clin Chim Acta. 1964 Feb;9:192–194. doi: 10.1016/0009-8981(64)90018-x. [DOI] [PubMed] [Google Scholar]
  15. LUSH I. E. Genetic polymorphisms in the egg albumen proteins of the domestic fowl. Nature. 1961 Mar 25;189:981–984. doi: 10.1038/189981a0. [DOI] [PubMed] [Google Scholar]
  16. MARSHALL R. D., NEUBERGER A. Amide nitrogen content of ovomucoid. Nature. 1960 Apr 23;186:311–312. doi: 10.1038/186311a0. [DOI] [PubMed] [Google Scholar]
  17. MONTREUIL J., CASTIGLIONI B., ADAM-CHOSSON A., CANER F., QUEVAL J. ETUDES SUR LES GLYCOPROT'EIDES. 8. L'H'ET'EROG'EN'EIT'E DE L'OVOMUCOUIDE. ETUDE CRITIQUE DES M'ETHODES DE PR'EPARATION DE L'OVOMUCOUIDE. J Biochem. 1965 Apr;57:514–528. doi: 10.1093/oxfordjournals.jbchem.a128110. [DOI] [PubMed] [Google Scholar]
  18. NEUBERGER A., PAPKOFF H. Carbohydrates in protein. 7. The nature of the carbohydrate in ovomucoid. Biochem J. 1963 Jun;87:581–585. doi: 10.1042/bj0870581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. NOBLE E., LEGAULT-DEMARE J., JUTISZ M. Absence de groupement C-terminal libre dans l'ovomucoide. Bull Soc Chim Biol (Paris) 1957 Sep 26;39(9-10):1077–1083. [PubMed] [Google Scholar]
  20. RAM J. S., TERMINIELLO L., BIER M., NORD F. F. On the mechanism of enzyme action. LVII. Interaction between trypsin and ovomucoid. Arch Biochem Biophys. 1954 Oct;52(2):451–463. doi: 10.1016/0003-9861(54)90145-9. [DOI] [PubMed] [Google Scholar]
  21. RHODES M. B., AZARI P. R., FEENEY R. E. Analysis, fractionation, and purification of egg white proteins with cellulose-cation exchanger. J Biol Chem. 1958 Jan;230(1):399–408. [PubMed] [Google Scholar]
  22. RUSSELL B., MEAD T. H., POLSON A. A METHOD OF PREPARING AGAROSE. Biochim Biophys Acta. 1964 Apr 4;86:169–174. doi: 10.1016/0304-4165(64)90171-0. [DOI] [PubMed] [Google Scholar]
  23. SMITHIES O. An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem J. 1959 Mar;71(3):585–587. doi: 10.1042/bj0710585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SMITHIES O. Molecular size and starch-gel electrophoresis. Arch Biochem Biophys. 1962 Sep;Suppl 1:125–131. [PubMed] [Google Scholar]
  25. SMITHIES O. Zone electrophoresis in starch gels and its application to studies of serum proteins. Adv Protein Chem. 1959;14:65–113. doi: 10.1016/s0065-3233(08)60609-9. [DOI] [PubMed] [Google Scholar]
  26. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  28. WISE R. W., KETTERER B., HANSEN I. A. PRE-ALBUMINS OF EMBRYONIC CHICK PLASMA. Comp Biochem Physiol. 1964 Jul;12:439–443. doi: 10.1016/0010-406x(64)90072-6. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES