Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Jul;104(1):78–82. doi: 10.1042/bj1040078

The electrophoresis of histones in polyacrylamide gel and their quantitative determination

E W Johns 1
PMCID: PMC1270547  PMID: 6035526

Abstract

1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2·4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative determination of the separated histone fractions by measuring the colour yields of dye–histone complexes formed in the gel.

Full text

PDF
82

Images in this article

80-1Plate 1 (a)
on p.80-1
80-1Plate 1 (b)
on p.80-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butler J. A., Power D. F., Palau J. Countercurrent-distribution studies on histones. Biochem J. 1967 Feb;102(2):539–547. doi: 10.1042/bj1020539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHN P., SIMSON P. BASIC AND OTHER PROTEINS IN MICROSOMES OF RAT LIVER. Biochem J. 1963 Aug;88:206–212. doi: 10.1042/bj0880206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. HNILICA L. S., BUSCH H. Fractionation of the histones of the Walker 256 carcinosarcoma by combined chemical and chromatographic techniques. J Biol Chem. 1963 Mar;238:918–924. [PubMed] [Google Scholar]
  5. HNILICA L., JOHNS E. W., BUTLER J. A. Observations on the species and tissue specificity of histones. Biochem J. 1962 Jan;82:123–129. doi: 10.1042/bj0820123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hjertén S., Jerstedt S., Tiselius A. Some aspects of the use of "continuous" and "discontinuous" buffer systems in polyacrylamide gel electrophoresis. Anal Biochem. 1965 May;11(2):219–223. doi: 10.1016/0003-2697(65)90008-4. [DOI] [PubMed] [Google Scholar]
  7. Hnilica L. S., Edwards L. J., Hey A. E. Studies on nuclear proteins. II. Quantitative distribution of histone fractions in various tissues. Biochim Biophys Acta. 1966 Jul 27;124(1):109–117. doi: 10.1016/0304-4165(66)90318-7. [DOI] [PubMed] [Google Scholar]
  8. JOHNS E. W., PHILLIPS D. M., SIMSON P., BUTLER J. A. The electrophoresis of histones and histone fractions on starch gel. Biochem J. 1961 Jul;80:189–193. doi: 10.1042/bj0800189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johns E. W. Studies on histones. 7. Preparative methods for histone fractions from calf thymus. Biochem J. 1964 Jul;92(1):55–59. doi: 10.1042/bj0920055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LAURENCE D. J., BUTLER J. A. METABOLISM OF HISTONES IN MALIGNANT TISSUES AND LIVER OF THE RAT AND MOUSE. Biochem J. 1965 Jul;96:53–62. doi: 10.1042/bj0960053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NEIDLE A., WAELSCH H. HISTONES: SPECIES AND TISSUE SPECIFICITY. Science. 1964 Sep 4;145(3636):1059–1061. doi: 10.1126/science.145.3636.1059. [DOI] [PubMed] [Google Scholar]
  12. PHILLIPS D. M., JOHNS E. W. A FRACTIONATION OF THE HISTONES OF GROUP F2A FROM CALF THYMUS. Biochem J. 1965 Jan;94:127–130. doi: 10.1042/bj0940127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PHILLIPS D. M. The presence of acetyl groups of histones. Biochem J. 1963 May;87:258–263. doi: 10.1042/bj0870258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RASMUSSEN P. S., MURRAY K., LUCK J. M. On the complexity of calf thymus histone. Biochemistry. 1962 Jan;1:79–89. doi: 10.1021/bi00907a013. [DOI] [PubMed] [Google Scholar]
  15. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  16. Shepherd G. R., Gurley L. R. High-resolution disc electrophoresis of histones. I. An improved method. Anal Biochem. 1966 Mar;14(3):356–363. doi: 10.1016/0003-2697(66)90278-8. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES