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. 1967 Jul;104(1):254–257. doi: 10.1042/bj1040254

Isolation of deoxyribonucleic acid from mammalian tissues

K S Kirby 1, E A Cook 1
PMCID: PMC1270569  PMID: 4291998

Abstract

1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol–cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides remained in solution when DNA was precipitated with 2-butoxyethanol in the presence of 0·5m-sodium chloride and 1·5m-sodium benzoate. 4. Ribosomal RNA was removed by precipitation in the presence of 3m-sodium chloride at 0°, when DNA remained soluble.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Frearson P. M., Kirby K. S. Nucleoproteins. 2. Fractionation of deoxyribonucleoproteins into acidic proteins and lysine-rich histones associated with deoxyribonucleic acid. Biochem J. 1964 Mar;90(3):578–583. doi: 10.1042/bj0900578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hastings J. R., Kirby K. S. The nucleic acids of Drosophila melanogaster. Biochem J. 1966 Aug;100(2):532–539. doi: 10.1042/bj1000532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KIRBY K. S. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem J. 1957 Jul;66(3):495–504. doi: 10.1042/bj0660495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KIRBY K. S. Induction of tumours by tannin extracts. Br J Cancer. 1960 Mar;14:147–150. doi: 10.1038/bjc.1960.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KIRBY K. S. Preparation of some deoxyribonucleic acid-protein complexes from rat-liver homogenates. Biochem J. 1958 Oct;70(2):260–265. doi: 10.1042/bj0700260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KIRBY K. S. The preparation of deoxyribonucleic acids by the p-aminosalicylate-phenol method. Biochim Biophys Acta. 1959 Nov;36:117–124. doi: 10.1016/0006-3002(59)90075-7. [DOI] [PubMed] [Google Scholar]
  8. Kirby K. S., Fox-Carter E., Guest M. Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochem J. 1967 Jul;104(1):258–262. doi: 10.1042/bj1040258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'SULLIVAN M. A., KIRBY K. S. STUDIES ON NUCLEIC ACIDS DURING CARCINOGENESIS. Br J Cancer. 1964 Dec;18:792–800. doi: 10.1038/bjc.1964.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Parish J. H., Kirby K. S. Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim Biophys Acta. 1966 Dec 21;129(3):554–562. doi: 10.1016/0005-2787(66)90070-0. [DOI] [PubMed] [Google Scholar]
  11. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]

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