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. 1967 Aug;104(2):404–409. doi: 10.1042/bj1040404

Fractionation of a hyaluronic acid preparation in a density gradient

The isolation and identification of a chondroitin sulphate

Panee Silpananta 1, J R Dunstone 1, A G Ogston 1
PMCID: PMC1270600  PMID: 6048782

Abstract

1. Following the suggestion of Nichol, Ogston & Preston (1967) that hyaluronic acid, prepared by filtration from ox synovial fluid, contains a component of high density, such material has been detected and isolated by equilibrium sedimentation in a density gradient of caesium chloride. 2. This material (fraction III) has been characterized as a chondroitin sulphate–protein complex of average molecular weight about 250000. Its amino acid pattern is characteristic of such complexes present in cartilage. It contains a proportion of glucosamine (as well as galactosamine); this is not due to contamination with hyaluronic acid. 3. Preliminary findings on fraction I (low density) and fraction II (intermediate density) suggest that these consist chiefly of protein and hyaluronic acid respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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