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. 1967 Aug;104(2):663–668. doi: 10.1042/bj1040663

A study of three enzymes acting on glucose in the lens of different species

Patricia K Pottinger 1,*
PMCID: PMC1270634  PMID: 6048806

Abstract

1. The activities of three enzymes which act on glucose, namely hexokinase, aldose reductase and glucose dehydrogenase, were measured in extracts of eye lens from cow, calf, rabbit, rat and guinea pig, and in human cataractous lenses. 2. The Km (glucose) of these three enzymes in extracts of cow lens was found to be 0·12mm, 28mm and 690mm respectively. 3. The physiological importance of hexokinase, aldose reductase and glucose dehydrogenase in the lens of normal and diabetic animals is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALLARD F. J. GLUCOSE UTILIZATION IN MAMMALIAN LIVER. Comp Biochem Physiol. 1965 Mar;14:437–443. doi: 10.1016/0010-406x(65)90218-5. [DOI] [PubMed] [Google Scholar]
  2. BECK W. S. The control of leukocyte glycolysis. J Biol Chem. 1958 May;232(1):251–270. [PubMed] [Google Scholar]
  3. GREEN H., BOCHER C. A., LEOPOLD I. H. Anaerobic carbohydrate metabolism of the crystalline lens. I. Glucose and glucose-6-phosphate. Am J Ophthalmol. 1955 Feb;39(2 Pt 2):106–113. doi: 10.1016/0002-9394(55)90014-3. [DOI] [PubMed] [Google Scholar]
  4. GREEN H., SOLOMON S. A. Hexokinase of rabbit lenses; effect of age of animal. AMA Arch Ophthalmol. 1959 Apr;61(4):616–625. doi: 10.1001/archopht.1959.00940090618017. [DOI] [PubMed] [Google Scholar]
  5. HAYMAN S., KINOSHITA J. H. ISOLATION AND PROPERTIES OF LENS ALDOSE REDUCTASE. J Biol Chem. 1965 Feb;240:877–882. [PubMed] [Google Scholar]
  6. KINOSHITA J. H., FUTTERMAN S., SATOH K., MEROLA L. O. FACTORS AFFECTING THE FORMATION OF SUGAR ALCOHOLS IN OCULAR LENS. Biochim Biophys Acta. 1963 Aug 13;74:340–350. doi: 10.1016/0006-3002(63)91377-5. [DOI] [PubMed] [Google Scholar]
  7. KINOSHITA J. H., MEROLA L. O., DIKMAK E. Osmotic changes in experimental galactose cataracts. Exp Eye Res. 1962 Jun;1:405–410. doi: 10.1016/s0014-4835(62)80030-x. [DOI] [PubMed] [Google Scholar]
  8. KINOSHITA J. H., MEROLA L. O., DIKMAK E. The accumulation of dulcitol and water in rabbit lens incubated with galactose. Biochim Biophys Acta. 1962 Jul 30;62:176–178. doi: 10.1016/0006-3002(62)90508-5. [DOI] [PubMed] [Google Scholar]
  9. KINOSHITA J. H., WACHTL C. A study of the C14-glucose metabolism of the rabbit lens. J Biol Chem. 1958 Jul;233(1):5–7. [PubMed] [Google Scholar]
  10. KLETHI J., MANDEL P. [Di- and triphosphopyridine nucleotides (DPN and TPN) from crystalline lens of young and old bovine animals]. Biochim Biophys Acta. 1960 Jan 29;37:549–550. doi: 10.1016/0006-3002(60)90522-9. [DOI] [PubMed] [Google Scholar]
  11. KUCK J. F., Jr SUGAR AND SUGAR ALCOHOL LEVELS IN THE AGING RAT LENS. Invest Ophthalmol. 1963 Dec;2:607–611. [PubMed] [Google Scholar]
  12. Klethi J., Mandel P. Eye lens nucleotides of different species of vertebrates. Nature. 1965 Mar 13;205(976):1114–1115. doi: 10.1038/2051114a0. [DOI] [PubMed] [Google Scholar]
  13. METZGER R. P., WILCOX S. S., WICK A. N. STUDIES WITH RAT LIVER GLUCOSE DEHYDROGENASE. J Biol Chem. 1964 Jun;239:1769–1772. [PubMed] [Google Scholar]
  14. METZGER R. P., WILCOX S. S., WICK A. N. SUBCELLULAR DISTRIBUTION AND PROPERTIES OF HEPATIC GLUCOSE DEHYDROGENASES OF SELECTED VERTEBRATES. J Biol Chem. 1965 Jul;240:2767–2771. [PubMed] [Google Scholar]
  15. NORDMANN J., MANDEL P. Enzyme studies in radiation cataract. Am J Ophthalmol. 1955 Dec;40(6):871–876. doi: 10.1016/0002-9394(55)91118-1. [DOI] [PubMed] [Google Scholar]
  16. PIRIE A., VANHEYNINGEN R. THE EFFECT OF DIABETES ON THE CONTENT OF SORBITOL, GLUCOSE, FRUCTOSE AND INOSITOL IN THE HUMAN LENS. Exp Eye Res. 1964 Jun;3:124–131. doi: 10.1016/s0014-4835(64)80027-0. [DOI] [PubMed] [Google Scholar]
  17. SIPPEL T. O. Pyridine nucleotides in normal and diabetic rat lenses. Exp Eye Res. 1962 Jun;1:368–371. doi: 10.1016/s0014-4835(62)80026-8. [DOI] [PubMed] [Google Scholar]
  18. SOLS A., CRANE R. K. Substrate specificity of brain hexokinase. J Biol Chem. 1954 Oct;210(2):581–595. [PubMed] [Google Scholar]
  19. STRECKER H. J., KORKES S. Glucose dehydrogenase. J Biol Chem. 1952 May;196(2):769–784. [PubMed] [Google Scholar]
  20. VAN HEYNINGEN R. Metabolism of xylose by the lens; calf lens in vitro. Biochem J. 1958 Aug;69(4):481–491. doi: 10.1042/bj0690481a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. VAN HEYNINGEN R., PHIL D., PIRIE A. Hexokinase in x-ray cataract in rabbits. Am J Ophthalmol. 1957 Jun;43(6):893–898. doi: 10.1016/0002-9394(57)91792-0. [DOI] [PubMed] [Google Scholar]
  22. Van Heyningen R. Some glycolytic enzymes and intermediates in the rabbit lens. Exp Eye Res. 1965 Dec;4(4):298–301. doi: 10.1016/s0014-4835(65)80044-6. [DOI] [PubMed] [Google Scholar]

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