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. 1967 Sep;104(3):852–860. doi: 10.1042/bj1040852

Transport of monosaccharides in kidney-cortex cells

A Kleinzeller 1, J Kolínská 1,*, I Beneš 1
PMCID: PMC1271224  PMID: 6049927

Abstract

1. The aerobic accumulation of various monosaccharides in slices of rabbit kidney cortex at 25° was studied. 2. d-Fructose and α-methyl d-glucoside were readily accumulated against their concentration gradient by a phlorrhizin-sensitive Na+-dependent active transport. In the absence of external Na+ the maximal rate of α-methyl glucoside transport was decreased tenfold, the Km of entry into the cells (8·2mm) not being affected. Phlorrhizin and d-galactose inhibited the entry of α-methyl glucoside also in the absence of external Na+. 3. d-Xylose, 6-deoxy-d-glucose and 6-deoxy-d-galactose were poorly accumulated ([S]i/[S]o ratios slightly above 1·0); this transport was inhibited by phlorrhizin and by the absence of Na+. 4. 3-O-Methyl-d-glucose, d-arabinose and l-arabinose were not actively transported, [S]i/[S]o ratios never exceeding 1·0. 5. 2-Deoxy-d-glucose and 2-deoxy-d-galactose were readily accumulated against a high concentration gradient, this transport being Na+-independent and only slightly sensitive to phlorrhizin. External Na+ was not required for an inhibitory action of phlorrhizin and d-galactose on the entry of 2-deoxy-d-galactose into the cells. 6. Interference for entry into the cells between the following saccharides was found: d-galactose inhibited α-methyl d-glucoside transport; d-xylose entry was inhibited by d-glucose; d-galactose transport was inhibited by d-xylose; a mutual interference between d-galactose and its 2-deoxy analogue was found. 7. It is concluded that d-glucose, d-galactose, α-methyl d-glucoside, d-xylose and possibly also some other monosaccharides share a common active transport system. 8. The specificity of the Na+-dependent phlorrhizin-sensitive active transport system for monosaccharides in kidney-cortex cells differs from that in intestinal epithelial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alvarado F. The relationship between Na+ and the active transport of arbutin in the small intestine. Biochim Biophys Acta. 1965 Nov 29;109(2):478–494. doi: 10.1016/0926-6585(65)90173-1. [DOI] [PubMed] [Google Scholar]
  2. Alvarado F. Transport of sugars and amino acids in the intestine: evidence for a common carrier. Science. 1966 Feb 25;151(3713):1010–1013. doi: 10.1126/science.151.3713.1010. [DOI] [PubMed] [Google Scholar]
  3. CRANE R. K. Intestinal absorption of sugars. Physiol Rev. 1960 Oct;40:789–825. doi: 10.1152/physrev.1960.40.4.789. [DOI] [PubMed] [Google Scholar]
  4. CRANE R. K., KRANE S. M. On the mechanism of the intestinal absorption of sugars. Biochim Biophys Acta. 1956 Jun;20(3):568–569. doi: 10.1016/0006-3002(56)90361-4. [DOI] [PubMed] [Google Scholar]
  5. CSAKY T. Z., RIGOR B. M., Sr A CONCENTRATIVE MECHANISM FOR SUGARS IN THE CHOROID PLEXUS. Life Sci. 1964 Sep;3:931–936. doi: 10.1016/0024-3205(64)90101-8. [DOI] [PubMed] [Google Scholar]
  6. Campbell P. N., Davson H. Absorption of 3-methylglucose from the small intestine of the rat and the cat. Biochem J. 1948;43(3):426–429. doi: 10.1042/bj0430426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crane R. K., Forstner G., Eichholz A. Studies on the mechanism of the intestinal absorption of sugars. X. An effect of Na+ concentration on the apparent Michaelis constants for intestinal sugar transport, in vitro. Biochim Biophys Acta. 1965 Nov 29;109(2):467–477. doi: 10.1016/0926-6585(65)90172-x. [DOI] [PubMed] [Google Scholar]
  8. Crane R. K. Na+ -dependent transport in the intestine and other animal tissues. Fed Proc. 1965 Sep-Oct;24(5):1000–1006. [PubMed] [Google Scholar]
  9. KLEINZELLER A., KOTYK A. Cations and transport of galactose in kidney-cortex slices. Biochim Biophys Acta. 1961 Dec 9;54:367–369. doi: 10.1016/0006-3002(61)90383-3. [DOI] [PubMed] [Google Scholar]
  10. KREBS H. A., BENNETT D. A., DE GASQUET P., GASQUET P., GASCOYNE T., YOSHIDA T. Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices. Biochem J. 1963 Jan;86:22–27. doi: 10.1042/bj0860022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KREBS H. A. Body size and tissue respiration. Biochim Biophys Acta. 1950 Jan;4(1-3):249–269. doi: 10.1016/0006-3002(50)90032-1. [DOI] [PubMed] [Google Scholar]
  12. Kleinzeller A., Kolínská J., Benes I. Transport of glucose and galactose in kidney-cortex cells. Biochem J. 1967 Sep;104(3):843–851. doi: 10.1042/bj1040843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Krebs H. A., Lund P. Formation of glucose from hexoses, pentoses, polyols and related substances in kidney cortex. Biochem J. 1966 Jan;98(1):210–214. doi: 10.1042/bj0980210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. THIER S., FOX M., ROSENBERG L., SEGAL S. HEXOSE INHIBITION OF AMINO ACID UPTAKE IN THE RAT-KIDNEY-CORTEX SLICE. Biochim Biophys Acta. 1964 Oct 9;93:106–115. doi: 10.1016/0304-4165(64)90265-x. [DOI] [PubMed] [Google Scholar]
  15. VOGEL G., LAUTERBACH F., KROEGER W. DIE BEDEUTUNG DES NATRIUMS FUER DIE RENALEN TRANSPORTE VON GLUCOSE UND PARA-AMINOHIPPURSAEURE. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965 Mar 18;283:151–159. [PubMed] [Google Scholar]
  16. WARAVDEKAR V. S., SASLAW L. D. A method of estimation of 2-deoxyribose. Biochim Biophys Acta. 1957 May;24(2):439–439. doi: 10.1016/0006-3002(57)90224-x. [DOI] [PubMed] [Google Scholar]
  17. WILSON T. H., CRANE R. K. The specificity of sugar transport by hamster intestine. Biochim Biophys Acta. 1958 Jul;29(1):30–32. doi: 10.1016/0006-3002(58)90142-2. [DOI] [PubMed] [Google Scholar]

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