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. 1967 Sep;104(3):960–969. doi: 10.1042/bj1040960

The microbial oxidation of methanol

The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group

C Anthony 1,*, L J Zatman 1
PMCID: PMC1271238  PMID: 6049934

Abstract

1. The purified alcohol dehydrogenase of Pseudomonas sp. M27, whose action is independent of nicotinamide nucleotides, has absorption peaks at 280mμ and at 350mμ with little or no absorption at or above 450mμ. 2. It does not fluoresce, but green-fluorescent material, diffusible on dialysis, is produced when the enzyme is treated with acid or alkali or when it is boiled. 3. Evidence is presented that the enzyme is not a flavoprotein. 4. Kinetic studies show a correlation between enzyme inactivation by acid, alkali or heat and liberation of the fluorescent material. 5. Some purification of the fluorescent material was achieved, but definite identification was not possible; the major component has a fluorescence maximum at about 460mμ with excitation maxima at about 260mμ and 365mμ. 6. Data are given (including absorption and fluorescence spectra) that support the suggestion that the prosthetic group of the enzyme is a pteridine derivative. 7. Possible mechanisms of action of the enzyme are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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