Abstract
Calves were inoculated at four week intervals with infectious bovine rhinotracheitis virus (IBRV). Sera were obtained at eight days (early sample) and 21 days (late sample) after each inoculation. Kinetic neutralization was carried out with 7S and 19S globulins derived from these sera, both in the presence and in the absence of guinea pig complement (C). In all instances the 19S neutralizing antibody (AB) was dependent on C for neutralization of IBVR. However, C dependency was not observed with any of the 7S preparations. Neutralizing activity was readily detected in 19S globulins from the early sera after primary and subsequent inoculations but not in any of the late sera. Early sera collected after primary inoculation did not contain any 7S neutralizing AB but it was present in all the other sera tested. The 7S AB when present was always at a considerably higher concentration than 19S AB. Thus it may be possible to determine whether cattle have recently been exposed to IBRV when paired serum samples are not available by determining the presence of C-dependent 19S globulins. In addition, by comparing 19S and 7S levels, a distinction may be made between primary and secondary responses to IBRV.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hampar B., Martos L. M., Ablashi D. V., Siguënza R. F., Wells G. A. Differentiation of cross-reacting simian cytomegalovirus strains by late 19S rabbit neutralizing antibodies. J Immunol. 1969 Nov;103(5):1155–1156. [PubMed] [Google Scholar]
- Hampar B., Miyamoto K., Martos L. M. Serologic classification of Herpes simplex viruses. J Immunol. 1971 Feb;106(2):580–582. [PubMed] [Google Scholar]
- Hampar B., Notkins A. L., Mage M., Keehn M. A. Heterogeneity in the properties of 7 S and 19S rabbit-neutralizing antibodies to herpes simplex virus. J Immunol. 1968 Mar;100(3):586–593. [PubMed] [Google Scholar]
- KENDRICK J. W., GILLESPIE J. H., MCENTEE K. Infectious pustular vulvovaginitis of cattle. Cornell Vet. 1958 Oct;48(4):458–495. [PubMed] [Google Scholar]
- Linscott W. D., Levinson W. E. Complement components required for virus neutralization by early immunoglobulin antibody. Proc Natl Acad Sci U S A. 1969 Oct;64(2):520–527. doi: 10.1073/pnas.64.2.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McBRIDE W. D. Antigenic analysis of polioviruses by kinetic studies of serum neutralization. Virology. 1959 Jan;7(1):45–58. doi: 10.1016/0042-6822(59)90176-x. [DOI] [PubMed] [Google Scholar]
- Potgieter L. N., Maré C. J. Differentiation of strains of infectious bovine rhinotracheitis virus by neutralization kinetics with late 19S rabbit antibodies. Infect Immun. 1974 Sep;10(3):520–527. doi: 10.1128/iai.10.3.520-527.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rossi C. R., Kiesel G. K. Complement-requiring neutralizing antibodies in cattle to infectious bovine rhinotracheitis virus. Arch Gesamte Virusforsch. 1974;45(4):328–334. doi: 10.1007/BF01242876. [DOI] [PubMed] [Google Scholar]
- Sheffy B. E., Davies D. H. Reactivation of a bovine herpesvirus after corticosteroid treatment. Proc Soc Exp Biol Med. 1972 Jul;140(3):974–976. doi: 10.3181/00379727-140-36592. [DOI] [PubMed] [Google Scholar]
- Stevens D. A., Pincus T., Burroughs M. A., Hampar B. Serologic relationship o a simian Herpes virus (SA8) and Herpes simplex virus: heterogeneity in the degree of reciprocal cross-reactivity shown by rabbit 7S and 19S antibodies. J Immunol. 1968 Nov;101(5):979–983. [PubMed] [Google Scholar]
- Straub O. C., Wizigmann G. Die Laboratoriumsdiagnose der Infektiösen Bovinen Rhinotracheitis (IBR) Berl Munch Tierarztl Wochenschr. 1968 Mar 15;81(6):108–109. [PubMed] [Google Scholar]