Skip to main content
PMC home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1976 Jan;40(1):46–52.

Studies on the response of ewes to live chlamydiae adapted to chicken embryos or tissue culture.

V M Becerra, F A Ata, J Storz
PMCID: PMC1277517  PMID: 1000377

Abstract

Ewes infected before gestation with chicken embryo or tissue culture adapted chlamydial strain B-577 were challenge inoculated with the homologous strain at four to 18 weeks of gestation. The ewes responsed with group specific complement fixing antibody titers of 1:8 to 1:256 by the second week after initial infection. A secondary antibody response in the surviving challenge inoculated ewes occurred at the time of lambing and reached titers of 1:32 to 1:256 by the second week after parturition. Group specific complement fixing antibodies did not appear to play a significant role in resistance to chlamydial infection. Ewes infected with the chicken embryo adapted strain B-577 excreted chlamydiae in their feces 60 days after inoculation. However, chlamydiae were not recovered from feces of ewes infected with the tissue culture adapted strain B-577. Placentas of ewes challenge inoculated by the intravenous route were consistently infected. Chlamydiae were recovered from placentas, some fetuses and lambs. In two instances when challenge inoculation was given by the intramuscular route, infection was detected only by the direct fluorescent antibody method.

Full text

PDF
49

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ata F. A., Storz J. Chlamydial blood clearance in convalescent sheep. Cornell Vet. 1974 Jan;64(1):25–36. [PubMed] [Google Scholar]
  2. BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becerra V. M., Storz J. Studies on conditions of interaction between chlamydiae and cultured animal cells. Zentralbl Bakteriol Orig. 1970;214(2):250–258. [PubMed] [Google Scholar]
  4. Eugster A. K., Joyce B. K., Storz J. Immunofluorescence studies on the pathogenesis of intestinal chlamydial infections in calves. Infect Immun. 1970 Oct;2(4):351–359. doi: 10.1128/iai.2.4.351-359.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eugster A. K., Storz J. Pathogenetic events in intestinal chlamydial infections leading to polyarthritis in calves. J Infect Dis. 1971 Jan;123(1):41–50. doi: 10.1093/infdis/123.1.41. [DOI] [PubMed] [Google Scholar]
  6. GIMENEZ D. F. STAINING RICKETTSIAE IN YOLK-SAC CULTURES. Stain Technol. 1964 May;39:135–140. doi: 10.3109/10520296409061219. [DOI] [PubMed] [Google Scholar]
  7. McEWEN A. D., STAMP J. T., LITTLEJOHN A. I. Enzootic abortion in ewes. II. Immunization and infection experiments. Vet Rec. 1951 Mar 17;63(11):197–201. doi: 10.1136/vr.63.11.197. [DOI] [PubMed] [Google Scholar]
  8. McKercher D. G., Crenshaw G. L., Theis J. H., Wada E. M., Mauris C. M. Experimentally induced immunity to chlamydial abortion of cattle. J Infect Dis. 1973 Aug;128(2):231–234. doi: 10.1093/infdis/128.2.231. [DOI] [PubMed] [Google Scholar]
  9. McKercher D. G., Robinson E. A., Wada E. M., Saito J. K., Franti C. E. Vaccination of cattle against epizootic bovine abortion. Cornell Vet. 1969 Apr;59(2):210–226. [PubMed] [Google Scholar]
  10. Philip R. N., Frank F. W., Lackman D. B., Price D. A., Casper E. A., Meinershagen W. A. Lambing performance of Idaho sheep vaccinated against naturally occurring enzootic abortion in ewes. Am J Vet Res. 1968 Jun;29(6):1153–1159. [PubMed] [Google Scholar]
  11. STORZ J. SUPERINFECTION OF PREGNANT EWES LATENTLY INFECTED WITH A PSITTACOSIS-LYMPHOGRANULOMA AGENT. Cornell Vet. 1963 Oct;53:469–480. [PubMed] [Google Scholar]
  12. Schoop G., Wachendörfer G., Krüger-Hansen-Schoop, Berger J. Erfahrungen mit einer Lebendvakzine zur Bekämpfung des Miyagawanellenabortes der Schafe. Zentralbl Veterinarmed B. 1968 Mar;15(2):209–223. [PubMed] [Google Scholar]
  13. Storz J., Marriott M. E., Thornley W. R. The dynamics of the blood infectious phase in psittacosis-induced abortions in animals. J Infect Dis. 1968 Jun;118(3):333–339. doi: 10.1093/infdis/118.3.333. [DOI] [PubMed] [Google Scholar]
  14. Storz J. Psittacosis-lymphogranuloma infection of sheep. Antigenic structures and interrelations of PL agents associated with polyarthritis, enzootic abortion, intrauterine and latent intestinal infections. J Comp Pathol. 1966 Oct;76(4):351–362. doi: 10.1016/0021-9975(66)90055-7. [DOI] [PubMed] [Google Scholar]
  15. Studdert M. J., McKercher D. G. Bedsonia abortion of sheep. 3. Immunological studies. Res Vet Sci. 1968 Jul;9(4):331–336. [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES