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. 1977 Oct;41(4):357–363.

Transmissible gastroenteritis: demonstration of the virus from field specimens by means of cell culture and pig inoculation.

G C Dulac, G M Ruckerbauer, P Boulanger
PMCID: PMC1277731  PMID: 200316

Abstract

Isolation of transmissible gastroenteritis virus was attempted from segments of jejunum collected from piglets submitted for diagnosis of transmissible gastroenteritis. The virus was isolated more frequently in susceptible piglets than in pig kidney or pig thyroid cells. Practically, both cell systems were equally capable of demonstrating the virus when the tissue suspensions were sonicated. The pig thyroid cells prepared with glands collected from minimal disease pigs were preferred to the pig kidney cells for initial virus isolation because of their ability to respond to transmissible gastroenteritis virus with a progressive cytopathic effect. However, the pig thyroid cells, prepared from pool of glands collected in abattoirs, were often contaminated with parvoviruses and could not be used for diagnostic work. Controlled ultrasound treatments of the inoculum increased the frequency of virus isolation in both cell systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boulanger P., Bannister G. L., Gray D. P., Ruckerbauer G. M., Willis N. G. African swine fever. II. Detection of the virus in swine tissues by means of the modified direct complement-fixation test. Can J Comp Med Vet Sci. 1967 Jan;31(1):7–11. [PMC free article] [PubMed] [Google Scholar]
  2. Boulanger P., Bannister G. L., Greig A. S., Gray D. P., Ruckerbauer G. M., Willis N. G. African swine fever. IV. Demonstration of the viral antigen by means of immunofluorescence. Can J Comp Med Vet Sci. 1967 Jan;31(1):16–23. [PMC free article] [PubMed] [Google Scholar]
  3. Cartwright S. F., Harris H. M., Blandford T. B., Fincham I., Gitter M. A cytopathic virus causing a transmissible gastroenteritis in swine. I. Isolation and properties. J Comp Pathol. 1965 Oct;75(4):387–396. doi: 10.1016/0021-9975(65)90019-8. [DOI] [PubMed] [Google Scholar]
  4. Cartwright S. F., Lucas M., Huck R. A. A small haemagglutinating porcine DNA virus. I. Isolation and properties. J Comp Pathol. 1969 Jul;79(3):371–377. doi: 10.1016/0021-9975(69)90053-x. [DOI] [PubMed] [Google Scholar]
  5. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dulac G. C., Boulanger P., Phaneuf J. B. Isolement du virus de la gastro-entérite transmissible du porc sur cultures cellulaires et comparaisons antigéniques avec deux souches américaines. Can Vet J. 1975 Mar;16(3):77–81. [PMC free article] [PubMed] [Google Scholar]
  7. Greig A. S., Boulanger P., Bannister G. L. African swine fever. V. Cultivation of the virus in primary pig kidney cells. Can J Comp Med Vet Sci. 1967 Jan;31(1):24–31. [PMC free article] [PubMed] [Google Scholar]
  8. Huygelen C., Peetermans J. Isolation of a hemagglutinating picornavirus from a primary swine kidney cell culture. Arch Gesamte Virusforsch. 1967;20(2):260–262. doi: 10.1007/BF01241281. [DOI] [PubMed] [Google Scholar]
  9. MCCLURKIN A. W. STUDIES ON TRANSMISSIBLE GASTROENTERITIS OF SWINE. I. THE ISOLATION AND IDENTIFICATION OF A CYTOPATHOGENIC VIRUS OF TRANSMISSIBLE GASTROENTERITIS IN PRIMARY SWINE KIDNEY CELL CULTURES. Can J Comp Med Vet Sci. 1965 Feb;29:46–53. [PMC free article] [PubMed] [Google Scholar]
  10. Mayr A., Bachmann P. A., Siegl G., Mahnel H., Sheffy B. E. Characterization of a small porcine DNA virus. Arch Gesamte Virusforsch. 1968;25(1):38–51. doi: 10.1007/BF01243088. [DOI] [PubMed] [Google Scholar]
  11. Mengeling W. L. Porcine parvovirus: properties and prevalence of a strain isolated in the United States. Am J Vet Res. 1972 Nov;33(11):2239–2248. [PubMed] [Google Scholar]
  12. Pensaert M., Haelterman E. O., Burnstein T. Transmissible gastroenteritis of swine: virus-intestinal cell interactions. I. Immunofluorescence, histopathology and virus production in the small intestine through the course of infection. Arch Gesamte Virusforsch. 1970;31(3):321–334. doi: 10.1007/BF01253767. [DOI] [PubMed] [Google Scholar]
  13. Sprino P. J., Morilla A., Ristic M. Intestinal immune response of feeder pigs to infection with transmissible gastroenteritis virus. Am J Vet Res. 1976 Feb;37(2):171–175. [PubMed] [Google Scholar]
  14. Witte K. H., Easterday B. C. Isolation and propagation of the virus of transmissible gastroenteritis of pigs in various pig cell cultures. Arch Gesamte Virusforsch. 1967;20(3):327–350. doi: 10.1007/BF01241952. [DOI] [PubMed] [Google Scholar]
  15. Witte K. H. Isolation of the virus of transmissible gastroenteritis (TGE) from naturally infected piglets in cell culture. Zentralbl Veterinarmed B. 1971 Dec;18(10):770–778. doi: 10.1111/j.1439-0450.1971.tb01654.x. [DOI] [PubMed] [Google Scholar]
  16. Witte K. H. Micro-color test for assay of transmissible gastroenteritis virus-neutralizing antibodies. Arch Gesamte Virusforsch. 1971;33(1):171–176. doi: 10.1007/BF01254174. [DOI] [PubMed] [Google Scholar]

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