Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1979 Jul;292:339–350. doi: 10.1113/jphysiol.1979.sp012854

Two-carrier influx of neutral amino acids into rabbit ileal mucosa.

J Y Paterson, F V Sepúlveda, M W Smith
PMCID: PMC1280861  PMID: 490361

Abstract

1. The influx of serine, alanine and methionine across the brush border membrane of the rabbit ileal mucosa has been measured during short periods of incubation. 2. A kinetic analysis of the uptake data, assuming one mediated entry mechanism or one mediated entry mechanism plus a diffusion component to be present, does not provide an adequate explanation for the results obtained. Methionine inhibition of serine uptake provided direct evidence that the diffusive entry of serine into the rabbit ileum was small or non-existent. 3. Data taken from amino acid inhibition and substrate-uptake experiments, fitted simultaneously to a double hyperbolic model of amino acid uptake, give good agreement between predicted and experimental results. There is also good quantitative agreement between computer-derived kinetic constants in the present work and similar constants obtained previously using a different method of analysis. 4. Present work supports the general hypothesis that neutral amino acids use two mediated pathways to enter the rabbit ileal mucosa. The possible physiological significance of these results and their probable effect on currently held concepts of how amino acids cross the brush border membrane of the rabbit intestinal mucosa is discussed.

Full text

PDF
339

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antonioli J. A., Joseph C., Robinson J. W. Kinetics of the absorption of amino acids by the rat intestine in vivo. Biochim Biophys Acta. 1978 Sep 11;512(1):172–191. doi: 10.1016/0005-2736(78)90228-6. [DOI] [PubMed] [Google Scholar]
  2. Atkins G. L., Gardner M. L. The computation of saturable and linear components of intestinal and other transport kinetics. Biochim Biophys Acta. 1977 Jul 4;468(1):127–145. doi: 10.1016/0005-2736(77)90156-0. [DOI] [PubMed] [Google Scholar]
  3. Bliss C. I., James A. T. Fitting the rectangular hyperbola. Biometrics. 1966 Sep;22(3):573–602. [PubMed] [Google Scholar]
  4. Christensen H. N., Handlogten M. E. Does the non-saturable cell entry apply to the charge-free form of amino acids? Biochim Biophys Acta. 1977 Sep 5;469(2):216–220. doi: 10.1016/0005-2736(77)90184-5. [DOI] [PubMed] [Google Scholar]
  5. Christensen H. N., Liang M. On the nature of the "non-saturable" migration of amino acids into Ehrlich cells and into rat jejunum. Bibl Laeger. 1966 Mar 14;112(3):524–531. doi: 10.1016/0926-6585(66)90255-x. [DOI] [PubMed] [Google Scholar]
  6. Curran P. F., Schultz S. G., Chez R. A., Fuisz R. E. Kinetic relations of the Na-amino acid interaction at the mucosal border of intestine. J Gen Physiol. 1967 May;50(5):1261–1286. doi: 10.1085/jgp.50.5.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Munck B. G., Schultz S. G. Interactions between leucine and lysine transport in rabbit ileum. Biochim Biophys Acta. 1969 Jun 3;183(1):182–193. doi: 10.1016/0005-2736(69)90142-4. [DOI] [PubMed] [Google Scholar]
  8. NEWEY H., SMYTH D. H. THE TRANSFER SYSTEM FOR NEUTRAL AMINO ACIDS IN THE RAT SMALL INTESTINE. J Physiol. 1964 Mar;170:328–343. doi: 10.1113/jphysiol.1964.sp007334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Peterson S. C., Goldner A. M., Curran P. F. Glycine transport in rabbit ileum. Am J Physiol. 1970 Oct;219(4):1027–1032. doi: 10.1152/ajplegacy.1970.219.4.1027. [DOI] [PubMed] [Google Scholar]
  10. Preston R. L., Schaeffer J. F., Curran P. F. Structure-affinity relationships of substrates for the neutral amino acid transport system in rabbit ileum. J Gen Physiol. 1974 Oct;64(4):443–467. doi: 10.1085/jgp.64.4.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schultz S. G., Markscheid-Kaspi L. Competitive interactions between L-alanine and L-phenylalanine in rabbit ileum. Biochim Biophys Acta. 1971 Sep 14;241(3):857–860. doi: 10.1016/0005-2736(71)90013-7. [DOI] [PubMed] [Google Scholar]
  12. Schultz S. G., Yu-Tu L., Strecker C. K. Influx of neutral amino acids across the brush border of rabbit ileum. Stereospecificity and the roles of the -amino and -carboxylate groups. Biochim Biophys Acta. 1972 Nov 2;288(2):367–379. doi: 10.1016/0005-2736(72)90258-1. [DOI] [PubMed] [Google Scholar]
  13. Sepúlveda F. V., Smith M. W. Different mechanisms for neutral amino acid uptake by new-born pig colon. J Physiol. 1979 Jan;286:479–490. doi: 10.1113/jphysiol.1979.sp012632. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES