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. 1979 Mar;288:411–423.

The effects of acetylcholine and dopamine on carotid chemosensory activity in the rabbit.

R J Docherty, D S McQueen
PMCID: PMC1281435  PMID: 469727

Abstract

1. Intracarotid (I.C.) injection of either acetylcholine (ACh) or dopamine inhibited spontaneous chemosensory activity recorded from the peripheral cut end of the sinus nerve in the anaesthetized rabbit. 2. High doses of ACh (greater than or equal to 50 micrograms I.C.) evoked a slight increase in discharge which preceded the inhibition. This excitation was attributable to a nicotinic action of the drug since it was abolished by mecamylamine. 3. The muscarinic agonist bethanechol inhibited chemoreceptor activity, an effect which was blocked by high doses of atropine, as was the inhibition caused by ACh. Dopamine-induced inhibition was unaffected by atropine. 4. Atropine, in doses sufficient to abolish the vasodepressor effect of ACh, only slightly reduced the inhibitory action of ACh on the chemoreceptors. Also, the vasodilators sodium nitrite and sodium nitroprusside did not appreciably alter chemosensory discharge. It seems unlikely, therefore, that the inhibitory response to ACh is secondary to vascular changes. 5. The inhibitory response to dopamine, but not that to ACh, was blocked by the dopamine antagonist alpha-flupenthixol. This implies that inhibition of chemosensory activity evoked by exogenous ACh was not secondary to dopamine release. 6. The implications of the results are discussed, particularly with regard to the possible physiological role of ACh as a modulator of carotid chemosensory activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMBACHE N. The use and limitations of atropine for pharmacological studies on autonomic effectors. Pharmacol Rev. 1955 Dec;7(4):467–494. [PubMed] [Google Scholar]
  2. BENNETT G., TYLER C., ZAIMIS E. Mecamylamine and its mode of action. Lancet. 1957 Aug 3;273(6988):218–222. doi: 10.1016/s0140-6736(57)91598-2. [DOI] [PubMed] [Google Scholar]
  3. Belmonte C., Eyzaguirre C. Efferent influences on carotid body chemoreceptors. J Neurophysiol. 1974 Nov;37(6):1131–1143. doi: 10.1152/jn.1974.37.6.1131. [DOI] [PubMed] [Google Scholar]
  4. Biscoe T. J. Carotid body: structure and function. Physiol Rev. 1971 Jul;51(3):437–495. doi: 10.1152/physrev.1971.51.3.437. [DOI] [PubMed] [Google Scholar]
  5. Biscoe T. J., Sampson S. R. Rhythmical and non-rhythmical spontaneous activity recorded from the central cut end of the sinus nerve. J Physiol. 1968 May;196(2):327–338. doi: 10.1113/jphysiol.1968.sp008510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Black A. M., Comroe J. H., Jr, Jacobs L. Species difference in carotid body response of cat and dog to dopamine and serotonin. Am J Physiol. 1972 Nov;223(5):1097–1102. doi: 10.1152/ajplegacy.1972.223.5.1097. [DOI] [PubMed] [Google Scholar]
  7. Brown G. L., Gray J. A. Some effects of nicotine-like substances and their relation to sensory nerve endings. J Physiol. 1948 Jun 25;107(3):306–317. doi: 10.1113/jphysiol.1948.sp004275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DARDYMOV I. V., GER B. A. PHARMACOLOGY OF CORCONIUM (SUBECHOLINE). Fed Proc Transl Suppl. 1964 Sep-Oct;23:917–920. [PubMed] [Google Scholar]
  9. Dearnaley D. P., Fillenz M., Woods R. I. The identification of dopamine in the rabbit's carotid body. Proc R Soc Lond B Biol Sci. 1968 Jun 11;170(1019):195–203. doi: 10.1098/rspb.1968.0033. [DOI] [PubMed] [Google Scholar]
  10. Docherty R. J., McQueen D. S. Inhibitory action of dopamine on cat carotid chemoreceptors. J Physiol. 1978 Jun;279:425–436. doi: 10.1113/jphysiol.1978.sp012354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Docherty R. J., McQueen D. S. Inhibitory effects of acetylcholine and dopamine on rabbit carotid chemoreceptors [proceedings]. J Physiol. 1978 Apr;277:64P–66P. [PubMed] [Google Scholar]
  12. Eyzaguirre C., Zapata P. The release of acetylcholine from carotid body tissues. Further study on the effects of acetylcholine and cholinergic blocking agents on the chemosensory discharge. J Physiol. 1968 Apr;195(3):589–607. doi: 10.1113/jphysiol.1968.sp008475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. GRAY J. A., DIAMOND J. Pharmacological properties of sensory receptors and their relation to those of the autonomic nervous system. Br Med Bull. 1957 Sep;13(3):185–188. doi: 10.1093/oxfordjournals.bmb.a069611. [DOI] [PubMed] [Google Scholar]
  14. Hellström S., Hanbauer I., Costa E. Selective decrease of dopamine content in rat carotid body during exposure to hypoxic conditions. Brain Res. 1976 Dec 17;118(2):352–355. doi: 10.1016/0006-8993(76)90725-3. [DOI] [PubMed] [Google Scholar]
  15. Henderson C. G., Ungar A. Antagonist affinity constants for adrenomedullary muscarinic receptors [proceedings]. Br J Pharmacol. 1977 Mar;59(3):499P–500P. [PMC free article] [PubMed] [Google Scholar]
  16. Hilton J. G. Acetylcholine stimulation of the sympathetic ganglia: effects of taurine and nicotinic and muscarinic ganglion block. J Pharmacol Exp Ther. 1977 Nov;203(2):426–434. [PubMed] [Google Scholar]
  17. Jacobs L., Comroe J. H., Jr Stimulation of the carotid chemoreceptors of the dog by dopamine. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1187–1193. doi: 10.1073/pnas.59.4.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Korner P. I., Uther J. B., White S. W. Circulatory effects of chloralose-urethane and sodium pentobarbitone anaesthesis in the rabbit. J Physiol. 1968 Dec;199(2):253–265. doi: 10.1113/jphysiol.1968.sp008652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. McCloskey D. I. Mechanisms of autonomic control of carotid chemoreceptor activity. Respir Physiol. 1975 Oct;25(1):53–61. doi: 10.1016/0034-5687(75)90050-x. [DOI] [PubMed] [Google Scholar]
  20. McQueen D. S. A quantitative study of the effects of cholinergic drugs on carotid chemoreceptors in the cat. J Physiol. 1977 Dec;273(2):515–532. doi: 10.1113/jphysiol.1977.sp012107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McQueen D. S. Effects of suberyldicholine on carotid baroreceptors and chemoreceptors. Neuropharmacology. 1974 Sep;13(9):829–835. doi: 10.1016/0028-3908(74)90038-0. [DOI] [PubMed] [Google Scholar]
  22. Neil E., O'Regan R. G. Effects of sinus and aortic nerve efferents on arterial chemoreceptor function. J Physiol. 1969 Jan;200(1):69P–71P. [PubMed] [Google Scholar]
  23. Nielsen I. M., Pedersen V., Nymark M., Franck K. F., Boeck V., Fjalland B., Christensen A. V. The comparative pharmacology of flupenthixol and some reference neuroleptics. Acta Pharmacol Toxicol (Copenh) 1973;33(5):353–362. doi: 10.1111/j.1600-0773.1973.tb01537.x. [DOI] [PubMed] [Google Scholar]
  24. STONE C. A., TORCHIANA M. L., NAVARRO A., BEYER K. H. Ganglionic blocking properties of 3-methylaminoisocamphane hydrochloride (mecamylamine): a secondary amine. J Pharmacol Exp Ther. 1956 Jun;117(2):169–183. [PubMed] [Google Scholar]
  25. Sampson S. R., Aminoff M. J., Jaffe R. A., Vidruk E. H. A pharmacological analysis of neurally induced inhibition of carotid body chemoreceptor activity in cats. J Pharmacol Exp Ther. 1976 Apr;197(1):119–125. [PubMed] [Google Scholar]
  26. Sampson S. R., Aminoff M. J., Jaffe R. A., Vidruk E. H. Analysis of inhibitory effect of dopamine on carotid body chemoreceptors in cats. Am J Physiol. 1976 Jun;230(6):1494–1498. doi: 10.1152/ajplegacy.1976.230.6.1494. [DOI] [PubMed] [Google Scholar]
  27. Sampson S. R. Effects of mecamylamine on responses of carotid body chemoreceptors in vivo to physiological and pharmacological stimuli. J Physiol. 1971 Feb;212(3):655–666. doi: 10.1113/jphysiol.1971.sp009348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sampson S. R. Mechanism of efferent inhibition of carotid body chemoreceptors in the cat. Brain Res. 1972 Oct 13;45(1):266–270. doi: 10.1016/0006-8993(72)90236-3. [DOI] [PubMed] [Google Scholar]
  29. Weiss S., Wilkins R. W., Haynes F. W. THE NATURE OF CIRCULATORY COLLAPSE INDUCED BY SODIUM NITRITE. J Clin Invest. 1937 Jan;16(1):73–84. doi: 10.1172/JCI100840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zapata P. Effects of dopamine on carotid chemo- and baroreceptors in vitro. J Physiol. 1975 Jan;244(1):235–251. doi: 10.1113/jphysiol.1975.sp010794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zapata P. Modulatory role of dopamine on arterial chemoreceptors. Adv Biochem Psychopharmacol. 1977;16:291–298. [PubMed] [Google Scholar]

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