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. 1978 Feb;275:241–262. doi: 10.1113/jphysiol.1978.sp012188

Calcium and strontium concentration changes within skinned muscle preparations following a change in the external bathing solution.

D G Moisescu, R Thieleczek
PMCID: PMC1282543  PMID: 24736

Abstract

1. A method for producing rapid [Ca2+] and [Sr2+] changes in the frog skinned muscle fibre preparation while maintaining constant all other cationic concentrations (Moisescu, 1976a, b) is described and analysed in detail. 2. Different experiments, some of them involving the Ca2+-sensitive photoprotein aequorin, as well as theoretical considerations, indicate that with this method one can produce a Ca2+ (or Sr2+) concentration change within 0.1--0.15 sec in a whole preparation having a diameter of 50 micrometer. 3. The rate of force development was similar to that observed in vivo. 4. The radial diffusion coefficient of EGTA in relaxed myofibrillar preparations was measured and found to be 4.6 x 10(-6) cm2sec-1 at 20 degrees C. 5. The sarcoplasmic reticulum in myofibrillar bundles was found to be active with respect to both Ca2+ and Sr2+ in the solutions used ([Mg2+] 1 mM; [Na] 30 mM; [K] 140-170 mM; [Cl] less than or equal to 20 mM; pH 7.10). 6. The amount of Ca released by caffeine from internal stores (previously loaded with Ca) can raise the total Ca concentration in the muscle fibre preparation by at least 1.8 mM. 7. The presence of 10 mM-caffeine in all bathing solutions reduced drastically the ability of the sarcoplasmic reticulum to accumulate both Ca and Sr.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADRIAN R. H. The effect of internal and external potassium concentration on the membrane potential of frog muscle. J Physiol. 1956 Sep 27;133(3):631–658. doi: 10.1113/jphysiol.1956.sp005615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ashley C. C., Ellory J. C. The efflux of magnesium from single crustacean muscle fibres. J Physiol. 1972 Nov;226(3):653–674. doi: 10.1113/jphysiol.1972.sp010002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ashley C. C., Moisescu D. G. Effect of changing the composition of the bathing solutions upon the isometric tension-pCa relationship in bundles of crustacean myofibrils. J Physiol. 1977 Sep;270(3):627–652. doi: 10.1113/jphysiol.1977.sp011972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ashley C. C., Moisescu D. G. Model for the action of calcium in muscle. Nat New Biol. 1972 Jun 14;237(76):208–211. doi: 10.1038/newbio237208a0. [DOI] [PubMed] [Google Scholar]
  5. Ashley C. C., Moisescu D. G. Proceedings: The influence of Mg2+ concentration and of pH upon the relationship between steady-state isometric tension and Ca2+ concentration in isolated bundles of barnacle myofibrils. J Physiol. 1974 Jun;239(2):112P–114P. [PubMed] [Google Scholar]
  6. Ashley C. C., Moisescu D. G. Tension changes in isolated bundles of frog and barnacle myofibrils in response to sudden changes in the external free calcium concentration. J Physiol. 1973 Aug;233(1):8P–9P. [PubMed] [Google Scholar]
  7. Blinks J. R., Prendergast F. G., Allen D. G. Photoproteins as biological calcium indicators. Pharmacol Rev. 1976 Mar;28(1):1–93. [PubMed] [Google Scholar]
  8. Blinks J. R., Prendergast F. G., Allen D. G. Photoproteins as biological calcium indicators. Pharmacol Rev. 1976 Mar;28(1):1–93. [PubMed] [Google Scholar]
  9. CALDWELL P. C. Studies on the internal pH of large muscle and nerve fibres. J Physiol. 1958 Jun 18;142(1):22–62. doi: 10.1113/jphysiol.1958.sp005998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Donaldson S. K., Kerrick W. G. Characterization of the effects of Mg2+ on Ca2+- and Sr2+-activated tension generation of skinned skeletal muscle fibers. J Gen Physiol. 1975 Oct;66(4):427–444. doi: 10.1085/jgp.66.4.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ebashi S., Endo M. Calcium ion and muscle contraction. Prog Biophys Mol Biol. 1968;18:123–183. doi: 10.1016/0079-6107(68)90023-0. [DOI] [PubMed] [Google Scholar]
  12. Endo M. Stretch-induced increase in activation of skinned muscle fibres by calcium. Nat New Biol. 1972 Jun 14;237(76):211–213. doi: 10.1038/newbio237211a0. [DOI] [PubMed] [Google Scholar]
  13. Endo M., Tanaka M., Ogawa Y. Calcium induced release of calcium from the sarcoplasmic reticulum of skinned skeletal muscle fibres. Nature. 1970 Oct 3;228(5266):34–36. doi: 10.1038/228034a0. [DOI] [PubMed] [Google Scholar]
  14. Fabiato A., Fabiato F. Calcium release from the sarcoplasmic reticulum. Circ Res. 1977 Feb;40(2):119–129. doi: 10.1161/01.res.40.2.119. [DOI] [PubMed] [Google Scholar]
  15. Ford L. E., Podolsky R. J. Calcium uptake and force development by skinned muscle fibres in EGTA buffered solutions. J Physiol. 1972 May;223(1):1–19. doi: 10.1113/jphysiol.1972.sp009830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ford L. E., Podolsky R. J. Intracellular calcium movements in skinned muscle fibres. J Physiol. 1972 May;223(1):21–33. doi: 10.1113/jphysiol.1972.sp009831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Godt R. E. Calcium-activated tension of skinned muscle fibers of the frog. Dependence on magnesium adenosine triphosphate concentration. J Gen Physiol. 1974 Jun;63(6):722–739. doi: 10.1085/jgp.63.6.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hellam D. C., Podolsky R. J. Force measurements in skinned muscle fibres. J Physiol. 1969 Feb;200(3):807–819. doi: 10.1113/jphysiol.1969.sp008723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. INFANTE A. A., KLAUPIKS D., DAVIES R. E. LENGTH, TENSION AND METABOLISM DURING SHORT ISOMETRIC CONTRACTIONS OF FROG SARTORIUS MUSCLES. Biochim Biophys Acta. 1964 Jul 29;88:215–217. doi: 10.1016/0926-6577(64)90171-8. [DOI] [PubMed] [Google Scholar]
  20. Inesi G., Scarpa A. [Fast kinetics of adenosine triphosphate dependent Ca 2+ uptake by fragmented sarcoplasmic reticulum]. Biochemistry. 1972 Feb 1;11(3):356–359. doi: 10.1021/bi00753a008. [DOI] [PubMed] [Google Scholar]
  21. Julian F. J. The effect of calcium on the force-velocity relation of briefly glycerinated frog muscle fibres. J Physiol. 1971 Oct;218(1):117–145. doi: 10.1113/jphysiol.1971.sp009607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Miller D. J., Moisescu D. G. The effects of very low external calcium and sodium concentrations on cardiac contractile strength and calcium-sodium antagonism. J Physiol. 1976 Jul;259(2):283–308. doi: 10.1113/jphysiol.1976.sp011466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moisescu D. G. Activation of isolated bundles of frog myofibrils in Ca-buffered solutions [proceedings]. J Physiol. 1976 Dec;263(1):161P–162P. [PubMed] [Google Scholar]
  24. Moisescu D. G., Ashley C. C. The effect of physiologically occurring cations upon aequorin light emission. Determination of the binding constants. Biochim Biophys Acta. 1977 May 11;460(2):189–205. doi: 10.1016/0005-2728(77)90206-7. [DOI] [PubMed] [Google Scholar]
  25. Moisescu D. G. Kinetics of reaction in calcium-activated skinned muscle fibres. Nature. 1976 Aug 12;262(5569):610–613. doi: 10.1038/262610a0. [DOI] [PubMed] [Google Scholar]
  26. Ogawa Y. Some properties of fragmented frog sarcoplasmic reticulum with particular reference to its response to caffeine. J Biochem. 1970 May;67(5):667–683. doi: 10.1093/oxfordjournals.jbchem.a129295. [DOI] [PubMed] [Google Scholar]
  27. Ogawa Y. The apparent binding constant of glycoletherdiaminetetraacetic acid for calcium at neutral pH. J Biochem. 1968 Aug;64(2):255–257. doi: 10.1093/oxfordjournals.jbchem.a128887. [DOI] [PubMed] [Google Scholar]
  28. Orentlicher M., Reuben J. P., Grundfest H., Brandt P. W. Calcium binding and tension development in detergent-treated muscle fibers. J Gen Physiol. 1974 Feb;63(2):168–186. doi: 10.1085/jgp.63.2.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. PORTZEHL H., CALDWELL P. C., RUEEGG J. C. THE DEPENDENCE OF CONTRACTION AND RELAXATION OF MUSCLE FIBRES FROM THE CRAB MAIA SQUINADO ON THE INTERNAL CONCENTRATION OF FREE CALCIUM IONS. Biochim Biophys Acta. 1964 May 25;79:581–591. doi: 10.1016/0926-6577(64)90224-4. [DOI] [PubMed] [Google Scholar]
  30. Podolsky R. J., Teichholz L. E. The relation between calcium and contraction kinetics in skinned muscle fibres. J Physiol. 1970 Nov;211(1):19–35. doi: 10.1113/jphysiol.1970.sp009263. [DOI] [PMC free article] [PubMed] [Google Scholar]

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