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. 1978 Aug;281:371–381. doi: 10.1113/jphysiol.1978.sp012428

The role of calcium in the receptor mediated control of potassium permeability in the rat lacrimal gland.

R J Parod, J W Putney Jr
PMCID: PMC1282703  PMID: 212554

Abstract

1. In the presence of extracellular Ca, adrenaline stimulated a large increase in the rate of K (86Rb) release from rat lacrimal slices, followed by a lower, more sustained rate. 2. In the absence of extracellular Ca, adrenaline elicited only a transient release of 86Rb. 3. The artificial introduction of Ca into the cytosol by the ionophore A-23187 could also initiate the release of 86Rb. 4. In a zero-Ca medium, if either adrenaline or carbachol produced a transient release of 86Rb, the tissue could not respond to the other agonist with a transient release unless Ca was momentarily reintroduced to the medium. 5. If Ca was present in a limiting concentration, the Ca-dependent rate of 86Rb release elicited from a lacrimal slice exposed simultaneously to carbachol and adrenaline was not significantly different from the release seen with carbachol alone. 6. It is concluded that the agonist-induced release of K from the lacrimal gland consists of both a Ca-independent phase which is initiated by the release of a limited pool of Ca, and a Ca-dependent phase which is mediated by the influx of extracellular Ca. 7. It is also concluded that both alpha-adrenergic and muscarinic receptor occupation activate a common, post-receptor mechanism which may be responsible for both phases of K release.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Deth R., van Breemen C. Agonist induced release of intracellular Ca2+ in the rabbit aorta. J Membr Biol. 1977 Jan 28;30(4):363–380. doi: 10.1007/BF01869677. [DOI] [PubMed] [Google Scholar]
  2. Deth R., van Breemen C. Relative contributions of Ca2+ influx and cellular Ca2+ release during drug induced activation of the rabbit aorta. Pflugers Arch. 1974 Apr 4;348(1):13–22. doi: 10.1007/BF00587735. [DOI] [PubMed] [Google Scholar]
  3. Douglas W. W., Rubin R. P. The mechanism of catecholamine release from the adrenal medulla and the role of calcium in stimulus-secretion coupling. J Physiol. 1963 Jul;167(2):288–310. doi: 10.1113/jphysiol.1963.sp007150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keryer G., Rossignol B. Effect of carbachol on 45Ca uptake and protein secretion in rat lacrimal gland. Am J Physiol. 1976 Jan;230(1):99–104. doi: 10.1152/ajplegacy.1976.230.1.99. [DOI] [PubMed] [Google Scholar]
  5. Koelz H. R., Kondo S., Blum A. L., Schulz I. Calcium ion uptake induced by cholinergic and alpha-adrenergic stimulation in isolated cells of rat salivary glands. Pflugers Arch. 1977 Jul 29;370(1):37–44. doi: 10.1007/BF00707943. [DOI] [PubMed] [Google Scholar]
  6. Kondo S., Schulz I. Calcium ion uptake in isolated pancreas cells induced by secretagogues. Biochim Biophys Acta. 1976 Jan 8;419(1):76–92. doi: 10.1016/0005-2736(76)90373-4. [DOI] [PubMed] [Google Scholar]
  7. Lew V. L. On the ATP dependence of the Ca 2+ -induced increase in K + permeability observed in human red cells. Biochim Biophys Acta. 1971 Jun 1;233(3):827–830. doi: 10.1016/0005-2736(71)90185-4. [DOI] [PubMed] [Google Scholar]
  8. Marier S. H., Putney J. W., Jr, Van de Walle C. M. Control of calcium channels by membrane receptors in the rat parotid gland. J Physiol. 1978 Jun;279:141–151. doi: 10.1113/jphysiol.1978.sp012336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Parod R. J., Putney J. W., Jr An alpha-adrenergic receptor mechanism controlling potassium permeability in the rat lacrimal gland acinar cell. J Physiol. 1978 Aug;281:359–369. doi: 10.1113/jphysiol.1978.sp012427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Putney J. W., Jr, Parod R. J., Marier S. H. Control by calcium of protein discharge and membrane permeability to potassium in the rat lacrimal gland. Life Sci. 1977 Jun 1;20(11):1905–1911. doi: 10.1016/0024-3205(77)90227-2. [DOI] [PubMed] [Google Scholar]
  11. Putney J. W., Jr Stimulation of 45Ca influx in rat parotid gland by carbachol. J Pharmacol Exp Ther. 1976 Dec;199(3):526–537. [PubMed] [Google Scholar]
  12. Putney J. W., Jr, Weiss S. J., Leslie B. A., Marier S. H. Is calcium the final mediator of exocytosis in the rat parotid gland? J Pharmacol Exp Ther. 1977 Oct;203(1):144–155. [PubMed] [Google Scholar]
  13. Romero P. J., Whittam R. The control by internal calcium of membrane permeability to sodium and potassium. J Physiol. 1971 May;214(3):481–507. doi: 10.1113/jphysiol.1971.sp009445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Selinger Z., Eimerl S., Schramm M. A calcium ionophore simulating the action of epinephrine on the alpha-adrenergic receptor. Proc Natl Acad Sci U S A. 1974 Jan;71(1):128–131. doi: 10.1073/pnas.71.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]

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