Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1980 Sep;306:579–586. doi: 10.1113/jphysiol.1980.sp013416

Intracellular potassium and sodium activities of chick ventricular muscle during embryonic development.

H A Fozzard, S S Sheu
PMCID: PMC1283025  PMID: 7463378

Abstract

1. The basis of the resting potential of chick embryo ventricular muscle was studied by use of ion-selective micro-electrodes. Membrane resting potential hyperpolarized from -65 . 4 +/- 1 . 1 mV (mean +/- S.E.) at age 4 day to -75 . 8 +/- 0 . 6 mV at age 18 day. Action potential overshoot increased from +19 . 8 +/- 0 . 9 at age 4 day to +33 . 1 +/- 0 . 6 mV at age 18 day. 2. Intracellular K+ activity measured with ion-selective micro-electrodes increased from 71 . 3 +/- 1 . 9 mM at age 4 day to 89 . 9 +/- 1 . 1 at age 18 day. Intracellular Na+ activity decreased from 12 . 5 +/- 0 . 4 to 7 . 0 +/- 0 . 3 mM during the same period. The difference between membrane resting potential and the calculated potassium equilibrium potential decreased with development. PNa/PK estimated from the constant field equation decreased from 0 . 012 at age 4 day to 0 . 005 at age 18 day. 3. The hyperpolarization of resting potential and the increased action potential overshoot during development could be explained by a rise in intracellular K+ activity and a fall in intracellular Na+ activity, as if the Na--K exchange pump became more active.

Full text

PDF
586

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carmeliet E. E., Horres C. R., Lieberman M., Vereecke J. S. Developmental aspects of potassium flux and permeability of the embryonic chick heart. J Physiol. 1976 Jan;254(3):673–692. doi: 10.1113/jphysiol.1976.sp011252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Couch J. R., West T. C., Hoff H. E. Development of the action potential of the prenatal rat heart. Circ Res. 1969 Jan;24(1):19–31. doi: 10.1161/01.res.24.1.19. [DOI] [PubMed] [Google Scholar]
  3. Ellis D. The effects of external cations and ouabain on the intracellular sodium activity of sheep heart Purkinje fibres. J Physiol. 1977 Dec;273(1):211–240. doi: 10.1113/jphysiol.1977.sp012090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KLEIN R. L. THE INDUCTION OF A TRANSFER ADENOSINE TRIPHOSPHATE PHOSPHOHYDROLASE IN EMBRYONIC CHICK HEART. Biochim Biophys Acta. 1963 Jul 9;73:488–498. doi: 10.1016/0006-3002(63)90450-5. [DOI] [PubMed] [Google Scholar]
  5. Kaplan J. G. Membrane cation transport and the control of proliferation of mammalian cells. Annu Rev Physiol. 1978;40:19–41. doi: 10.1146/annurev.ph.40.030178.000315. [DOI] [PubMed] [Google Scholar]
  6. Lee C. O., Armstrong W. M. State and distribution of potassium and sodium ions in frog skeletal muscle. J Membr Biol. 1974;15(4):331–362. doi: 10.1007/BF01870094. [DOI] [PubMed] [Google Scholar]
  7. Lee C. O., Fozzard H. A. Activities of potassium and sodium ions in rabbit heart muscle. J Gen Physiol. 1975 Jun;65(6):695–708. doi: 10.1085/jgp.65.6.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McDonald T. F., DeHaan R. L. Ion levels and membrane potential in chick heart tissue and cultured cells. J Gen Physiol. 1973 Jan;61(1):89–109. doi: 10.1085/jgp.61.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosen M. R., Hordof A. J., Hodess A. B., Verosky M., Vulliemoz Y. Ouabain-induced changes in electrophysiologic properties of neonatal, young and adult canine cardiac Purkinje Fibers. J Pharmacol Exp Ther. 1975 Jul;194(1):255–263. [PubMed] [Google Scholar]
  10. Shimizu Y., Tasaki K. Electrical excitability of developing cardiac muscle in chick embryos. Tohoku J Exp Med. 1966 Jan 25;88(1):49–56. doi: 10.1620/tjem.88.49. [DOI] [PubMed] [Google Scholar]
  11. Sperelakis N. (Na + , K + )-ATPase activity of embryonic chick heart and skeletal muscles as a function of age. Biochim Biophys Acta. 1972 Apr 14;266(1):230–237. doi: 10.1016/0005-2736(72)90137-x. [DOI] [PubMed] [Google Scholar]
  12. Sperelakis N., Shigenobu K. Changes in membrane properties of chick embryonic hearts during development. J Gen Physiol. 1972 Oct;60(4):430–453. doi: 10.1085/jgp.60.4.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tuganowski W., Cekański A. Electrical activity of a single fibre of the human embryonic heart. Pflugers Arch. 1971;323(1):21–26. doi: 10.1007/BF00586562. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES