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. 1980 Oct;307:429–442. doi: 10.1113/jphysiol.1980.sp013444

Effects of pentagastrin on intestinal absorption and blood flow in the anaesthetized dog.

D Mailman
PMCID: PMC1283054  PMID: 7205671

Abstract

1. Pentagastrin (1, 10 micrograms/min) was infused I.V. into fed and fasted anaesthetized dogs and the intestinal absorption of NaCl and H2O and blood flow were determined. The influence of pentagastrin-induced cardiovascular changes on absorption was investigated. 2. 22Na and 3H2O were used to determine the unidirectional Na and H2O fluxes from saline perfused through the ileal lumen and the clearances of 3H2O were used to calculate total and absorptive site blood flow. 3. Ileal absorption of Na and H2O was reduced by 10 micrograms/min pentagastrin due primarily to significant increases in the secretory flux of Na and decreases in the absorptive flux of H2O in both fed and fasted animals. 4. Neither total intestinal blood flow, arterial nor mesenteric vein pressure were changed by pentagastrin but absorptive site blood flow was decreased in fasted but not in fed dogs. 5. Pretreatment with atropine reduced the effects of pentagastrin but pretreatment with guanethidine potentiated the effects of pentagastrin. 6. Absorptive site blood flow was positively linearly correlated with the absorptive fluxes of both Na and H2O. The relationships between the secretory fluxes of Na and H2O and estimated capillary pressure were changed from a positive relationship in control periods to a less positive or negative relationship following pentagastrin. 7. It was concluded that pentagastrin reduces intestinal absorption through both a cardiovascular effect and an effect on the intestinal epithelium. Also, there is a strong autonomic component in the effects of pentagastrin on intestinal absorption.

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Selected References

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  1. BERGER E. Y., STEELE J. M. The calculation of transfer rates in two compartment systems not in dynamic equilibrium. J Gen Physiol. 1958 Jul 20;41(6):1135–1152. doi: 10.1085/jgp.41.6.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barbezat G. O., Grossman M. I. Cholera-like diarrhoea induced by glucagon plus gastrin. Lancet. 1971 May 15;1(7707):1025–1026. doi: 10.1016/s0140-6736(71)91428-0. [DOI] [PubMed] [Google Scholar]
  3. Barbezat G. O., Grossman M. I. Intestinal secretion: stimulation by peptides. Science. 1971 Oct 22;174(4007):422–424. doi: 10.1126/science.174.4007.422. [DOI] [PubMed] [Google Scholar]
  4. Bowen J. C., Pawlik W., Fang W. F., Jacobson E. D. Pharmacologic effects of gastrointestinal hormones on intestinal oxygen consumption and blood flow. Surgery. 1975 Oct;78(4):515–519. [PubMed] [Google Scholar]
  5. Bynum T. E., Jacobson E. D., Johnson L. R. Gastrin inhibition of intestinal absorption in dogs. Gastroenterology. 1971 Dec;61(6):858–862. [PubMed] [Google Scholar]
  6. Denniss A. R., Young J. A. Modification of salivary duct electrolyte transport in rat and rabbit by physalaemin, VIP, GIP and other enterohormones. Pflugers Arch. 1978 Aug 25;376(1):73–80. doi: 10.1007/BF00585250. [DOI] [PubMed] [Google Scholar]
  7. El Masri S. H., Lewin M. R., Clark C. G. In vitro effects of gastrin on the movement of electrolytes across the human colon. Scand J Gastroenterol. 1977;12(8):999–1002. doi: 10.3109/00365527709181364. [DOI] [PubMed] [Google Scholar]
  8. Folkow B. Regional adjustments of intestinal blood flow. Gastroenterology. 1967 Feb;52(2):423–432. [PubMed] [Google Scholar]
  9. Gardner J. D., Peskin G. W., Cerda J. J., Brooks F. P. Alterations of in vitro fluid and electrolyte absorption by gastrointestinal hormones. Am J Surg. 1967 Jan;113(1):57–64. doi: 10.1016/0002-9610(67)90257-7. [DOI] [PubMed] [Google Scholar]
  10. Gingell J. C., Davies M. W., Shields R. Effect of a synthetic gastrin-like pentapeptide upon the intestinal transport of sodium, potassium, and water. Gut. 1968 Feb;9(1):111–116. doi: 10.1136/gut.9.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hakim A. A., Lifson N. Effects of pressure on water and solute transport by dog intestinal mucosa in vitro. Am J Physiol. 1969 Feb;216(2):276–284. doi: 10.1152/ajplegacy.1969.216.2.276. [DOI] [PubMed] [Google Scholar]
  12. Hubel K. A. Intestinal ion transport: effect of norepinephrine, pilocarpine, and atropine. Am J Physiol. 1976 Jul;231(1):252–257. doi: 10.1152/ajplegacy.1976.231.1.252. [DOI] [PubMed] [Google Scholar]
  13. Isaacs P. E., Corbett C. L., Riley A. K., Hawker P. C., Turnberg L. A. In vitro behavior of human intestinal mucosa. The influence of acetyl choline on ion transport. J Clin Invest. 1976 Sep;58(3):535–542. doi: 10.1172/JCI108498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MacFerran S. N., Mailman D. Effects of glucagon on canine intestinal sodium and water fluxes and regional blood flow. J Physiol. 1977 Mar;266(1):1–12. doi: 10.1113/jphysiol.1977.sp011753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mailman D. Effects of vasoactive intestinal polypeptide on intestinal absorption and blood flow. J Physiol. 1978 Jun;279:121–132. doi: 10.1113/jphysiol.1978.sp012334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mailman D., Jordan K. The effect of saline and hyperoncotic dextran infusion on canine ileal salt and water absorption and regional blood flow. J Physiol. 1975 Oct;252(1):97–113. doi: 10.1113/jphysiol.1975.sp011136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Makhlouf G. M. The neuroendocrine design of the gut. The play of chemicals in a chemical playground. Gastroenterology. 1974 Jul;67(1):159–184. [PubMed] [Google Scholar]
  18. Vizi S. E., Bertaccini G., Impicciatore M., Knoll J. Evidence that acetylcholine released by gastrin and related polypeptides contributes to their effect on gastrointestinal motility. Gastroenterology. 1973 Feb;64(2):268–277. [PubMed] [Google Scholar]
  19. Winne D. The influence of blood flow and water net flux on the absorption of tritiated water from the jejunum of the rat. Naunyn Schmiedebergs Arch Pharmacol. 1972;272(4):417–436. doi: 10.1007/BF00501248. [DOI] [PubMed] [Google Scholar]
  20. Yau W. M., Makhlouf G. M. Different effects of hormonal peptides and cyclic adenosine 3',5'-monophosphate on colonic transport in vitro. Gastroenterology. 1974 Oct;67(4):662–667. [PubMed] [Google Scholar]
  21. ZOLLINGER R. M., ELLISON E. H. Primary peptic ulcerations of the jejunum associated with islet cell tumors of the pancreas. Ann Surg. 1955 Oct;142(4):709-23; discussion, 724-8. [PMC free article] [PubMed] [Google Scholar]

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