Abstract
Sex peptide (SP) transferred during mating induces female post-mating responses including refractoriness to re-mate and increased oviposition in Drosophila. Yet, where SP-target neurons reside remained uncertain. Here, we show that expression of membrane-tethered SP (mSP) predominantly in the head or trunk either reduces receptivity or increases oviposition, respectively. Using fragments from large regulatory regions of Sex Peptide Receptor, fruitless, and doublesex genes together with intersectional expression of mSP, we identified distinct interneurons in the brain and abdominal ganglion controlling receptivity and oviposition. These SP response-inducing neurons (SPRINz) can induce post-mating responses through SP received by mating. Trans-synaptic mapping of neuronal connections reveals input from sensory processing neurons and two post-synaptic trajectories as output. Hence, SP-target neurons operate as key integrators of sensory information for decision-making of behavioural outputs. Multi-modularity of SP-targets further allows females to adjust SP-mediated male manipulation to physiological state and environmental conditions for maximising reproductive success.
Research organism: D. melanogaster
Introduction
Reproductive behaviours are to a large degree hard-wired in the brain to guarantee reproductive success, making the underlying neuronal circuits amenable to genetic analysis (Dulac and Kimchi, 2007; Yamamoto and Koganezawa, 2013; Anderson, 2016; Rings and Goodwin, 2019).
During development, sex-specific circuits are built into the brain under the control of the sex determination genes doublesex (dsx) and fruitless (fru) in Drosophila (Schütt and Nöthiger, 2000; Billeter et al., 2006). They encode transcription factors that are alternatively spliced in a male or female-specific mode (Schütt and Nöthiger, 2000). By default, the dsx gene generates the male-specific isoform DsxM, while a female-specific isoform DsxF is generated by alternative splicing and expressed in about ~700 distinct neurons in the brain important for female reproductive behaviours directing readiness to mate and egg laying (Rideout et al., 2010; Rezával et al., 2012). FruM is expressed in about ~1000 neurons in males and implements development of neuronal circuitry key to display male courtship behaviour, but is switched off in females through alternative splicing by incorporation of a premature stop codon (Demir and Dickson, 2005; Manoli et al., 2005; Stockinger et al., 2005).
The circuitry of female-specific behaviours, including receptivity to courting males for mating and egg laying, has been mapped using intersectional gene expression via the split-GAL4 system to restrict expression of activators or inhibitors of neuronal activity to very few neurons (Aranha and Vasconcelos, 2018; Wang et al., 2020a; Wang et al., 2020b; Wang et al., 2021; Cury and Axel, 2023). Through this approach, sensory neurons in the genital tract have been identified as key signal transducers for the readiness to mate and the inhibition of egg laying connecting to central parts of the brain via projection to abdominal ganglion neurons (Häsemeyer et al., 2009; Yang et al., 2009; Rezával et al., 2012; Feng et al., 2014). This circuit then projects onto centrally localised pattern generators in the brain to direct a behavioural response via efferent neurons (Wang et al., 2020a; Wang et al., 2020b; Wang et al., 2021).
Once females have mated, they will reject courting males and lay eggs (Manning, 1967). Post-mating responses (PMRs) are induced by male-derived sex peptide (SP) and other substances transferred during mating (Chen et al., 1988; Avila et al., 2011; Hopkins and Perry, 2022; Kim et al., 2024; Singh and Soller, 2025). In addition to refractoriness to remate and oviposition, SP will induce a number of other behavioural and physiological changes, including increased egg production, feeding, a change in food choice, sleep, memory, constipation, midgut morphology, stimulation of the immune system, and sperm storage and release (Soller et al., 1999; Peng et al., 2005; Carvalho et al., 2006; Domanitskaya et al., 2007; Kim et al., 2010; Ribeiro and Dickson, 2010; Scheunemann et al., 2019; Cognigni et al., 2011; Avila et al., 2010; Isaac et al., 2010; Wainwright et al., 2021; White et al., 2021). SP binds to broadly expressed sex peptide receptor (SPR), an ancestral receptor for myoinhibitory peptides (MIPs) (Yapici et al., 2008; Kim et al., 2010; Jang et al., 2017). Although MIPs seem not to induce PMRs, excitatory activity of MIP-expressing neurons underlies re-mating (Yapici et al., 2008; Kim et al., 2010; Jang et al., 2017). Expression of membrane-tethered SP (mSP) induces PMRs in an autocrine fashion when expressed in neurons, but not glia (Nakayama et al., 1997; Haussmann et al., 2013).
First attempts to identify SP target neurons by enhancer GAL4-induced expression of UASmSP only identified lines with broad expression in the nervous system (Nakayama et al., 1997). Later, drivers with more restricted expression, including dsx, fru, and pickpocket (ppk) genes were identified, but they are expressed in all parts of the nervous system throughout the body, eluding to reveal the location of SP target sites unambiguously (Yapici et al., 2008; Häsemeyer et al., 2009; Yang et al., 2009; Rezával et al., 2012; Haussmann et al., 2013).
To delineate where in the Drosophila SP target neurons are located which induce the main PMRs, refusal to mate and egg laying, we expressed mSP predominantly in the head or trunk. These experiments separate reduction of receptivity induced in the head from trunk induction of egg laying. To further restrict our search for SP target neurons, we focused on three genes, SPR, dsx, and fru, because SPR is broadly expressed but anticipated to induce PMRs only from few neurons, and because GAL4 inserted in the endogenous dsx and fru loci induces PMRs from mSP expression. Using GAL4 tiling lines with fragments encompassing the regulatory regions of complex SPR, fru, and dsx genes (Pfeiffer et al., 2008; Jenett et al., 2012; Kvon et al., 2014), we identified one regulatory region in each gene reducing receptivity and inducing egg laying upon mSP expression, and one additional region in SPR only inducing egg laying. To further refine this analysis, we used intersectional gene expression using split-GAL4 and flipase (flp)-mediated excision of stop cassettes in UAS reporters (Struhl and Basler, 1993; Luan et al., 2006). Consistent with previous results that the SP response can be induced via multiple pathways (Haussmann et al., 2013), we found distinct sets of SP response-inducing neurons (SPRINz) in the central brain and the abdominal ganglion that can induce PMRs via expression of mSP either reducing receptivity and inducing egg laying, or affecting only one of these PMRs. In contrast, we identified genital tract neuron expressing lines including split-GAL4 nSyb ∩ ppk that did not induce PMRs by expression of mSP. Likewise, we find expression of mSP or neuronal activation in head sex peptide sensing neurons (SPSN) neurons can induce PMRs. Mapping the pre- and post-synaptic connections of the distinct SP target neurons by retro- and trans-Tango (Talay et al., 2017; Sorkaç et al., 2023) revealed that SP target neurons direct higher order sensory processing in the central brain. These neurons feed into two common post-synaptic neuronal subtypes indicating that SP interferes with the integration of diverse sensory inputs to build a stereotyped output either reducing receptivity and/or increasing egg laying.
Results
Reduction of receptivity and induction of egg laying are separable by head and trunk expression of membrane-tethered SP
Due to the complex behavioural and physiological changes induced by SP, neurons in the central nervous system have been suspected as main targets for SP (Kubli, 1992). To express mSP only in the head, we used an elav FRTstopFRT GAL4 in combination with otdflp that expresses in the head to drive recombination and head-specific expression of mSP from UAS (Figure 1A, Figure 1—figure supplement 1A–F; Haussmann et al., 2008; Asahina et al., 2014; Zaharieva et al., 2015; Nallasivan et al., 2021). To express mSP predominantly in the trunk, we used tshGAL4 (Figure 1B, Figure 1—figure supplement 1G–L; Soller et al., 2006).
Figure 1. The main post-mating responses (PMRs) in females can be separated.
(A, B) Schematic depiction of head and trunk expression in Drosophila elav FRTstopFRT GAL4; otdflp (A) and in tshGAL4 (B) visualised by UAS GFP (green). (C, D) Receptivity (C) and oviposition (D) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) pan-neuronally with nsybGAL4 or in head and trunk patterns shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001). (E–H) Representative adult female genital tract showing tshGAL4 UAS H2BYFP (green) and elavLexA LexAop NLStomato (red) nuclear expression. The magnification (F–H) shows sensory genital tract neurons. Scale bars shown in (E) and (H) are 100 μm and 20 μm, respectively.
Figure 1—figure supplement 1. Analysis of head and trunk expression lines.
When we expressed mSP in the head, females reduced receptivity indistinguishable from mated females, but did not lay eggs, thereby again demonstrating that the two main PMRs can be separated (Figure 1C and D; Haussmann et al., 2013). In contrast, when we expressed mSP in the trunk, females remained receptive but laid eggs in numbers indistinguishable from mated females (Figure 1C and D).
Moreover, tshGAL4 is expressed in fru, dsx, ppk genital tract sensory neurons (Figure 1E–H). Since mSP expression with tshGAL4 does not affect receptivity, these genital tract neurons unlikely are direct targets for SP (Haussmann et al., 2013). Taken together, these results indicate the presence of SP target neurons in the brain and ventral nerve cord (VNC) for the reduction of receptivity and induction of egg laying, respectively.
Few restricted regulatory regions in large SPR, fru, and dsx genes can induce the SP response
Expression of mSP from UAS via GAL4 inserts in fru and dsx genes induces a robust reduction in receptivity and increase in egg laying (Rezával et al., 2012; Haussmann et al., 2013). To identify SP target neurons, we thought to dissect the broad expression pattern of complex SPR, fru, and dsx genes spanning 50–80 kb by identifying regulatory DNA fragments in the enhancer regions that drive UAS mSP in a subset of neurons. For these experiments, we analysed 22, 27, and 25 GAL4 lines from the VDRC and Janelia tiling GAL4 projects (Pfeiffer et al., 2008; Jenett et al., 2012; Kvon et al., 2014; Figure 2A–C).
Figure 2. Distinct regulatory regions in SPR, fru, and dsx genes induce post-mating responses (PMRs) from mSP expression.
(A–C) Schematic representation of SPR, fru, and dsx chromosomal regions depicting coding and non-coding exons as black or white boxes, respectively, and splicing patterns in solid lines. Vertical lines below the gene model depict enhancer GAL4 lines with names and those in red showed PMRs by expression of mSP. (D, E) Receptivity (D) and oviposition (E) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of GAL4 pan-neuronally in nsyb or in SPR8, SPR12, fru11, fru12, and dsx24 patterns shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p≤0.0001). (F–O) Representative adult female brains (F–J) and ventral nerve cords (VNC, K–O) expressing UAS CD8GFP under the control of SPR8, SPR12, fru11, fru12, and dsx24 GAL4. Scale bars shown in (J) and (O) are 50 µm and 100 µm, respectively.
Figure 2—figure supplement 1. Expression analysis of PMR-inducing GAL4 in the genital tract.
Figure 2—figure supplement 2. Expression analysis of non-PMR-inducing fru9GAL4 in the genital tract.
Strikingly, in SPR, fru, and dsx genes, we identified only one regulatory region in each gene (SPR8, fru11/12, and dsx24) that reduced receptivity and induced egg laying through GAL4 UAS expression of mSP (Figure 2D and E). In addition, we identified one line (SPR12) in the SPR gene that induced egg laying but did not reduce receptivity, consistent with previous results that SP regulation of receptivity and egg laying can be split (Haussmann et al., 2013).
All of these lines expressed in subsets of neurons in the central brain and the VNC in distinct, but reduced patterns compared to the expression of the SPR, fru, and dsx genes (Yapici et al., 2008; Rideout et al., 2010; Zhou et al., 2014; Figure 2F–O). Moreover, these lines showed prominent labelling of abdominal ganglion neurons in the VNC (Figure 2K–O). In addition, all of these lines except SPR12 are also expressed in genital tract sensory neurons (Figure 2—figure supplement 1A–E).
From all the 74 lines that we have analysed for PMRs from SPR, fru, and dsx genes, we also analysed expression in genital tract sensory neurons as they had been postulated to be the primary targets of SP (Yapici et al., 2008; Häsemeyer et al., 2009; Yang et al., 2009; Rezával et al., 2012). Apart from PMR-inducing lines SPR8, fru11, fru12, and dsx24, that showed expression in genital tract sensory neurons, we identified three lines (SPR3, SPR 21, and fru9), which also robustly expressed in genital tract sensory neurons but did not induce PMRs from expression of mSP (Figure 3, Figure 2—figure supplement 2).
Figure 3. Expression of mSP in SPSN and genital tract expressing SPR lines does not support a major role for genital tract neurons in inducing the sex peptide response.
(A, B) Receptivity (A) and oviposition (B) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of SPSN 1 and SPSN2, and SPR3 and SPR9 GAL4 lines shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001). (C–J) Representative genital tracts labelled with UAS CD8 GFP and genital tract neurons labelled with UAS H2BYFP and elavLexA AopNLStomato. (K–R) Adult female brains (K–N) and ventral nerve cords (VNC. O–R) expressing UAS CD8GFP. Scale bars shown in (F, J, N, R) are 100 µm, 20 µm, 50 µm and 100 µm, respectively.
Genital tract neurons do not mediate changes in receptivity and oviposition by mSP
Since genital tract sensory neurons have been postulated to induce the SP response, we tested previously identified split-GAL4 (SPSN-1: VT058873 ∩ VT003280/FD6 and SPSN-2: VT58873 ∩ VT033490) lines, which upon neuronal inhibition reduced receptivity and induced egg laying (Feng et al., 2014), for their capacity to induce the SP response upon expression of mSP. Both lines reduced receptivity and induced egg laying upon expression of mSP (Figure 3A and B). However, since genital tract neuron expressing SPR3 and SPR21 lines did not induce PMRs upon expression of mSP, the SP response induced by SPSN1 and 2 split-GAL4 mSP expression could originate from other neurons.
Expression analysis of these two lines revealed that in addition to expression in genital tract sensory neurons (Figure 3C, D, G, and H), they also showed expression in the brain and VNC (Figure 3K, L, O, and P). Intriguingly, the brain neurons labelled in SPSN-1 resembled the neurons identified by SPR8 ∩ FD6 (Figure 4G).
Figure 4. Expression of membrane-tethered sex peptide (mSP) in secondary ascending abdominal ganglion neurons induces post-mating responses (PMRs).
(A, B) Receptivity (A) and oviposition (B) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of GAL4 pan-neuronally in nsyb or in FD1, FD2, FD3, FD4, FD5, and FD6, or with SAG split-Gal4 patterns shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001).
Secondary ascending abdominal ganglion neurons can induce the PMRs from mSP expression
A screen aiming to identify neurons involved in the control of receptivity and egg laying by expression of the rectifying potassium channel Kir2.1 identified six enhancer GAL4 driver lines (FD1-6) (Feng et al., 2014). FD1-6 are expressed in diverse subsets of neurons in the brain and the VNC; in particular, they show common expression in the abdominal ganglion with projections to the central brain. The lines expressing in FD1-5 neurons have been termed SAG (secondary ascending abdominal ganglion neurons) neurons that are also interconnected with MIP sensing neurons (Jang et al., 2017). Since enhancer lines identified in SPR, fru, and dsx genes are prominently expressed in the abdominal ganglion, we tested whether mSP expression from these FD1-6 lines induced PMRs.
From these six lines, one robustly suppressed receptivity and induced egg laying (FD6/VT003280), while two lines only induced egg laying (FD3/VT4515 and FD4/V000454) similar to controls from mSP expression (Figure 4). Again, all three lines also expressed in subsets of neurons in the central brain and VNC, particularly in the abdominal ganglion (Zhou et al., 2014). In addition, FD3 and FD4 did not express in genital tract sensory neurons, in contrast to FD6 (Feng et al., 2014). A SAG split-GAL4 (VT050405/FD1 AD and VT007068/FD2 DBD) line did not show a response to expression of mSP and virgin females, for example, they mated and did not lay eggs (Figure 4).
Intersectional expression reveals distinct mSP-responsive neurons in the central brain and abdominal ganglion
To further restrict the expression to fewer neurons, we intersected the expression patterns of those lines that induced robust reduction of receptivity and increase of egg laying using split-GAL4 (SPR8, fru11/12, dsx, and FD6; for further experiments we used dsxGAL4-DBD, because dsx24 is less robust and fru11 and fru12 were made into one fragment) that activates the UAS reporter when GAL4 is reconstituted via dimerisation of activation (AD-GAL4) and DNA binding (GAL4-DBD) domains (Luan et al., 2006; Figure 5A).
Figure 5. Distinct circuits from the intersection of SPR, fru, dsx, and FD6 patterns in the brain and ventral nerve cord (VNC) induce post-mating responses (PMRs) from membrane-tethered sex peptide (mSP) expression.
(A) Schematic showing the intersectional gene expression approach: GAL4 activation (AD, orange) and DNA binding domains (DBD, blue) are expressed in different, but overlapping patterns. Leucine zipper dimerisation reconstitutes a functional split-GAL4 in the intersection (pink) to express UAS reporters. (B, C) Receptivity (B) and oviposition (C) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of split-GAL4 intersecting SPR8 ∩ fru11/12, SPR8 ∩ dsx, SPR8 ∩ FD6, fru11/12 ∩ dsx, and fru11/12 ∩ FD6 patterns shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001). (D–M) Representative adult female brains and VNC expressing UAS CD8GFP under the control of SPR8 ∩ fru11/12, SPR8 ∩ dsx, SPR8 ∩ FD6, fru11/12 ∩ dsx, and fru11/12 ∩ FD6. Scale bars shown in (H) and (M) are 50 µm and 100 µm, respectively.
Figure 5—figure supplement 1. Expression analysis of split-GAL4 in the genital tract.
Again, the intersection of SPR8 with fru11/12, dsx or FD6, and fru11/12 with dsx or FD6 expression robustly reduced receptivity and increased egg laying upon expression of mSP (Figure 5B and C). Accordingly, we termed these SP response-inducing neurons SPRINz, though the exact identity in the split-GAL4 intersection population needs to be determined.
When we further analysed the expression of these split-GAL4 intersections in the brain, we found that each combination first showed very restricted expression, but second, that none of these combinations labelled the same neurons (Figure 5D–H). For dsx neurons, split-GAL4 intersections correspond to a subset of dPC2l (SPR8 ∩ dsx) and dPCd-2 (fru11/12 ∩ dsx) neurons (Deutsch et al., 2020; Schretter et al., 2020; Nojima et al., 2021). These results suggest the SP targets interneurons in the brain that feed into higher processing centres from different entry points likely representing different sensory input.
In the VNC, we found expression in the abdominal ganglion with all split-GAL4 combinations (Figure 5I–M). In particular, the intersection of dsx with SPR8 or fru11/12 showed exclusive expression in the abdominal ganglion, while the other combinations also expressed in other cells of the VNC. Altogether, these data suggest that the abdominal ganglion harbours several distinct types of neurons involved in directing PMRs (Oliveira-Ferreira et al., 2023).
In the female genital tract, these split-Gal4 combinations show expression in genital tract neurons with innervations running along oviduct and uterine walls (Figure 5—figure supplement 1A–J). In addition, SPR8 ∩ fru11/12 and SPR8 ∩ dsx were also expressed in the spermathecae (Figure 5—figure supplement 1A and B).
mSP-responsive neurons rely on SPR and are required for PMRs induced by SP delivered through mating
Next, we tested whether PMRs induced by mSP expression in the SPR8 ∩ dsx, fru11/12 ∩ dsx or SPR8 ∩ fru11/12 rely on SPR. Expression of mSP in dsx ∩ SPR8 and dsx ∩ fru11/12 neurons in SPR mutant females did not reduce receptivity or induce egg laying (Figure 6A and B, see also Figure 5A and B), while a partial response was observed for SPR8 ∩ fru 11/12 induced mSP expression in SPR mutant females, which is consistent with presence of additional receptors for SP (Haussmann et al., 2013).
Figure 6. Distinct neuronal circuitries from the intersection of SPR, fru, and dsx sense sex peptide (SP) after mating to induce post-mating responses (PMRs).
(A, B) Receptivity (A) and oviposition (B) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of split-Gal4 intersecting SPR8 ∩ dsx, fru11/12 ∩ dsx, and SPR8 ∩ fru11/12 patterns in SPR/Df mutant females or SPR RNAi knock-down shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001 except p=0.002 and p=0.006 for c and d in A, and P=0.004 for c in B).
Since SP is transferred during mating to females and enters the hemolymph (Haussmann et al., 2013), we wanted to test whether SPR is required in these neurons for inducing PMRs after mating. For SPR RNAi in dsx ∩ fru11/12 and SPR8 ∩ fru 11/12 neurons, no reduction, or a partial reduction, of receptivity was observed, respectively, while SPR RNAi in dsx ∩ SPR8 neurons turned virgin females unreceptive (Figure 6A). Expression of mSP in dsx ∩ fru11/12 neurons in the context of SPR RNAi partially reduced receptivity, again suggesting additional receptors for SP (Haussmann et al., 2013).
Strikingly, however, SPR RNAi in these neurons prevented egg laying independent of whether SP was delivered by mating or when tethered to the membrane of these neurons (Figure 6B).
These results demonstrate that neurons identified by split-GAL4 intersected expression of SPR8 with dsx or fru11/12, or fru11/12 with dsx are genuine SP targets as they rely on SPR and PMRs are induced by SP delivered through mating.
Expression of mSP in distinct neurons in the brain induces PMRs
The analysis of ppkGAL4 neurons in SP-insensitive Nup54 alleles revealed a hierarchy of trunk neurons that dominate over central brain neurons (Nallasivan et al., 2021). To focus on the role of central brain neurons, we generated a UAS mSP line with a stop cassette (UAS FRTstopFRT mSP) that allows us to restrict expression of mSP to the head in the presence of otdflp, which only expresses in the head (Figure 7A), but not in the trunk (Asahina et al., 2014; Nallasivan et al., 2021).
Figure 7. Distinct neuronal circuitries in the brain sense SP to induce post-mating responses (PMRs).
(A, B) Schematic depiction of UAS GFP (green) expression in the head of Drosophila (A) combining split-GAL4 intersectional expression (AD-GAL4 and GAL4-DBD) with brain-expressed otdflp mediated recombination of UAS FRTGFPstopFRTmSP (B). (C, D) Receptivity (C) and oviposition (D) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS FRTGFPstopFRTmSP (grey), UAS FRTGFPstopFRTTrpA1 (purple) and UAS FRTGFPstopFRTTNT (pink) under the control of split-GAL4 intersecting SPR8 ∩ dsx, fru11/12 ∩ dsx and SPR8 ∩ fru11/12 patterns with brain-specific FRT-mediated recombination by otdflp shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001 except p<0.0004 for c in C, p<0.007 for c in D).
In combination with the intersectional approach, we now can restrict mSP expression to few central brain neurons, or alternatively activate or silence these neurons (Figure 7B). Expression of mSP in SPR8 ∩ dsx, fru11/12 ∩ dsx, or SPR8 ∩ fru11/12 neurons in the central brain significantly reduced receptivity, but oviposition was only substantially induced in SPR8 ∩ dsx brain neurons (Figure 7C and D). In fru11/12 ∩ dsx or SPR8 ∩ fru11/12, PMR inducing neurons from the VNC could be required to potentiate the response.
These results clearly demonstrate a role for brain neurons in the SP response. However, we noticed that the flipase approach can result in false negatives as fruflp inserted in the same position in the endogenous locus as fruGAL4 does not induce a response with UAS FRTstopFRT mSP in contrast to fruGAL4-induced expression of mSP. In contrast, the same experiment with dsxGAL4 and dsxflp results in a positive SP response indistinguishable from mated females (Haussmann et al., 2013).
Next, we tested whether neuronal activation or inhibition would induce a PMR. Strikingly, conditional activation of SPR8 ∩ dsx, fru11/12 ∩ dsx, or SPR8 ∩ fru11/12 brain neurons with TrpA1 in adult females completely inhibited receptivity and induced egg laying comparable to mated females (Figure 7C and D). In contrast, inhibition of these neurons with tetanus toxin (TNT) did not alter the virgin state, for example, receptivity was not reduced and egg laying was not induced (Figure 7C and D).
Overlapping expression of SPSN with SPR8 and dsx mediates changes in receptivity and oviposition by mSP expression in the brain
When we analysed split-GAL4 combinations of SPSN (VT058873, the common line in the SPSN1 and 2 lines) with SPR8, fru11/12, and dsx, we observed full response to mSP expression for the intersection with SPR8 and fru11/12, and a partial response for the SPSN ∩ dsx intersection (Figure 8A and B). Intriguingly, all of these split-Gal4 combinations expressed in few neurons in the brain, the VNC and genital tract neurons, except for VT058873 ∩ fru11/12, which did not express in genital tract neurons (Figure 8C–N).
Figure 8. Expression of membrane-tethered sex peptide (mSP) in SPSN VT058873 AD intersected with SPR8 DBD, fru11/12 DBD, and dsx DBD induces post-mating responses (PMRs) and SPSN VT058873 AD intersected with SPR8 DBD and dsx DBD sense sex peptide (SP) in the brain.
(A, B) Receptivity (A) and oviposition (B) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of VT058873 ∩ SPR8, VT058873 ∩ fru11/12, and VT058873 ∩ dsx shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001). (C–H) Adult female brains (C–E) and ventral nerve cords (VNC, F–H) expressing UAS CD8GFP. Scale bars shown in (E, H, K, N) are 50 µm, 100 µm, 100 µm and 20 µm, respectively. (I–N) Representative genital tracts labelled with UAS CD8 GFP and genital tract neurons labelled with UAS H2BYFP and elavLexA AopNLStomato. (O, P) Receptivity (O) and oviposition (P) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS FRTGFPstopFRTmSP (grey) and UAS FRTGFPstopFRTTrpA1 (purple) under the control of split-GAL4 intersecting VT058873 ∩ SPR8, VT058873 ∩ fru11/12, and VT058873 ∩ dsx patterns with brain-specific FRT-mediated recombination by otdflp shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001).
Figure 8—figure supplement 1. Expression analysis of split-GAL4 in the genital tract.
We then restricted expression of mSP and induction of neuronal activity to the head with these split-GAL4 combinations using FRTstop cassettes and otdflp. In this set-up, we can induce PMRs from mSP expression or neuronal activation from TrpA1 expression with the VY058873 ∩ SPR8 and dsx combination, but not with the fru11/12 combination (Figure 8O and P). For the VT058873 ∩ fru11/12 intersection, PMR inducing neurons likely reside in the VNC.
We then analysed co-expression of SPR, dsx, and fru with SPSN originating split-GAL4 enhancer lines from CG31637 (FD6), ocelliless (VT05573), and Gyc76C (VT033490) in the single-cell brain atlas (Li et al., 2022). CG31637 co-expressed in many cells with SPR and fru, but only a few cells with dsx (Figure 8—figure supplement 1A–C). Expression of ocelliless with SPR and fru is broad, while only one neuron expressed with and Gyc76C in the brain (Figure 8—figure supplement 1D, E, G, and H). Expression of ocelliless with dsx is restricted to two neurons, and no overlap was detected with Gyc76C in the brain (Figure 8—figure supplement 1F and I).
ppk neurons do not intersect with SPR, fru, dsx, and FD6 neurons in inducing PMRs by mSP
Expression of UASmSP using a GAL4 driven by a promoter fragment of the ppk gene can also induce PMRs (Figure 9A and B; Häsemeyer et al., 2009; Yang et al., 2009). The complement of neurons labelled with ppkGAL4 consists of at least two populations including prominently sensory neurons, but also eight interneurons in the central brain (Nallasivan et al., 2021). These brain neurons show severe developmental defects in SP-insensitive Nup54 mutant alleles, but they receive inhibitory input from sensory neurons (Nallasivan et al., 2021).
Figure 9. ppk is not part of the SPR8, SPR12, and fru11/12 post-mating response (PMR)-inducing neuronal circuitry.
(A, B) Receptivity (A) and oviposition (B) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of GAL4 in ppk or in nSyb ∩ ppk, SPR8 ∩ ppk, SPR12 ∩ ppk, and fru11/12 ∩ ppk patterns shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001). (C–R) Representative adult female brains, ventral nerve cords (VNC) and genital tracts expressing UAS CD8GFP under the control of UAS by nSyb ∩ ppk, SPR8 ∩ ppk, SPR12 ∩ ppk, and fru11/12 ∩ ppk. Scale bars shown in (F, J, N, R) are 50 µm, 100 µm, 100 µm and 20 µm, respectively. (S–V) Receptivity (S, T) and oviposition (U, V) of wild type control virgin (red) and mated (orange) females, and virgin females expressing either UAS TNT (azure) or UAS NaChBac (brown) to inhibit or activate neurons in SPR8 ∩ ppk, SPR12 ∩ ppk, and fru11/12 ∩ ppk patterns shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.001 for b, and p<0.01 for c in L and N).
To evaluate whether ppkGAL4 neurons are part of the previously identified expression patterns, we intersected them by crossing GAL4-AD lines SPR8, SPR12 and fru11/12 and the pan-neural nSybAD with a ppk GAL4-DBD line containing the previously used 3 kb promoter fragment (Grueber et al., 2003; Seidner et al., 2015; Riabinina et al., 2019). Surprisingly, none of these split-GAL4 combinations reduced female receptivity or increased egg laying (Figure 9A and B).
Few GFP-expressing neurons were detected in the brain for the nSyb ∩ ppk and the fru11/12 ∩ ppk intersection (Figure 9C–F) or abdominal ganglion (Figure 9G–J). For the nSyb ∩ ppk and the SPR8 ∩ ppk intersection, we detected GFP expression in genital tract sensory neurons (Figure 9K, L, O, and P), but not for the other combinations (Figure 9M, N, Q, and R).
Inhibiting or activating neurons with these split-Gal4 combinations did not reduce receptivity or induce egg laying (Figure 9S–V). How exactly ppk neurons labelled with ppkGAL4 impact on PMRs, however, needs to be further evaluated in follow-up studies. Moreover, if genetical tract neurons were SP target sites, an SP response would have been expected for the nSyb ∩ ppk intersection, which we did not observe.
Female post-mating neuronal circuitry contains neurons that reduce receptivity without inducing oviposition in response to mSP
A number of additional split-GAL4 combinations with restricted expression have been identified that play a role in female reproductive behaviours (Wang et al., 2020a; Wang et al., 2020b; Wang et al., 2021). These lines express in a subset of dsx expressing neurons (pC1-SS1), in oviposition descending neurons (oviDN-SS1 and 2), in oviposition excitatory neurons (oviEN-SS1 and 2), in oviposition inhibitory neurons (oviIN-SS1 and 2), and in vaginal plate opening neurons (vpoDN-SS1, also termed ovipositor extrusion/rejection behaviour neurons, because Drosophila does not have vaginal plates like e.g. seen in Hemiptera; Aigaki et al., 1991; Soller et al., 2006). When we analysed these lines for a response to mSP expression, receptivity was reduced from mSP expression in oviEN-SS2, oviN-SS1, and vpoDN-SS1 neurons, but no egg laying was induced from mSP expression in any of these neurons (Figure 10A and B).
Figure 10. Expression of membrane-tethered sex peptide (mSP) in female reproductive behaviour regulating neuron split-GAL4 lines.
(A, B) Receptivity (A) and oviposition (B) of wild type control virgin (red) and mated (orange) females, and virgin females expressing UAS mSP (green) under the control of pC1-SS1, oviDN-SS1 and 2, oviEN-SS1 and 2, oviIN-SS1 and 2, and vpoDN-SS1 shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by different letters (p<0.0001). (C–J) Representative genital tract neurons labelled with UAS H2BYFP and elavLexA AopNLStomato. The scale bar shown in (J) is 20 µm.
In genital tract neurons, OviDN-SS1s, OviEN-SS1, OviIN-SS1, and vpoDNs express, but OviDN-SS1s and OviEN-SS1 express weakly (Figure 10C and J).
Interference with neuronal activity in SPRINz reveals regulatory hierarchy
Both inhibitory and activating neurons have been attributed to impact on PMRs (Kvitsiani and Dickson, 2006; Yapici et al., 2008; Rezával et al., 2012). These neurons seem to be part of intersecting circuitry as general inhibition of ppkGAL4 neurons by TNT only partially blocks the SP response in contrast to inhibition of ppkGAL4 neurons in the brain alone (Nallasivan et al., 2021).
When we inhibited neuronal activity by expression of TNT (Sweeney et al., 1995), we observed a significant reduction of receptivity for all split-Gal4 combinations, though only partially for inhibition in fru11/12 ∩ FD6 neurons. Likewise, all split-Gal4 combinations induced a significant increase in egg laying (Figure 11A and B). Ablation of these neurons by expression of apoptosis-inducing reaper and hid genes essentially replicated the results from neuronal inhibition indicating that SPR target neurons are modulatory and are not part of motor circuits because females laid eggs and performed normally in receptivity assays (Figure 11C and D).
Figure 11. Post-mating responses (PMRs) after neuronal inhibition, ablation, or activation of distinct circuits from intersection of SPR, fru, dsx, and FD6 patterns in the brain and ventral nerve cord (VNC).
(A–F) Receptivity (A, C, E) and oviposition (B, D, F) of wild type control virgin (red) and mated (orange) females, and virgin females expressing either UAS TNT (azure, A, B) or UAS reaper hid to inhibit or ablate neurons (yellow, C, D), respectively, or UAS NaChBac (brown, E, F) to activate neurons in SPR8 ∩ fru11/12, SPR8 ∩ dsx, SPR8 ∩ FD6, fru11/12 ∩ dsx, and fru11/12 ∩ FD6 split-Gal4 patterns shown as means with standard error from three repeats for receptivity (21 females per repeat) by counting the number of females mating within a 1-hour period or for oviposition by counting the eggs laid within 18 hours from 30 females. Statistically significant differences from ANOVA post hoc comparison are indicated by letters (p≤0.0095 in A, B, p<0.0001 in C, D except p=0.016 for c in D, p<0.0001 in E and p<0.0002 in F).
To evaluate the composition of the intersected expression patterns into inhibitory and activating neurons, we also expressed the Bacillus halodurans sodium channel (NaChBac) (Feng et al., 2014) to activate all of the intersected neurons. Here, we found a significant reduction of receptivity for four of the five split-GAL4 combinations, though only partially for activation of SPR8 ∩ dsx neurons (Figure 11E). Activating fru11/12 ∩ FD6 neurons did not reduce receptivity (Figure 11E). Likewise, we found the same pattern for the induction of egg laying (Figure 11F). Four of the five split-GAL4 combinations induced a significant increase which was only partial in SPR8 ∩ dsx neurons, and no egg laying was induced by activating fru11/12 ∩ FD6 neurons.
Essentially, these results are consistent with previous findings that inhibitory neurons prevail (Nallasivan et al., 2021), possibly as input from trunk neurons as found for ppk expressing neurons.
mSP-responsive neurons operate in higher order sensory processing in the brain
With the split-GAL4 approach, we identified five distinct neuronal sub-types that can induce PMRs. To find out whether these neurons receive input from distinct entry points in the brain and to identify the target neurons of these mSP-responsive neurons, we used the retro- and trans-Tango technique to specifically activate reporter gene expression in up- and down-stream neurons (Talay et al., 2017; Sorkaç et al., 2023; Figure 12A–O).
Figure 12. retro- and trans-Tango identification of pre- and post-synaptic neurons of SP target neurons reveals higher order neuronal input canalised into shared output circuitries.
(A–O) Representative adult female brains expressing QUAST tomato3xHA retro-Tango (left, A–E), UAS myrGFP (middle, F–J) and QUAST tomato3xHA trans-Tango (right, K–O) in SPR8 ∩ dsx, fru11/12 ∩ dsx, SPR8 ∩ fru11/12, SPR8 ∩ FD6, and fru11/12 ∩ FD6 split-GAL4s. The presynaptic (A–E, left), split-GAL4 (F–J, middle) and postsynaptic (K–O, right) neuronal circuitries are shown in an inverted grey background. Arrows (magenta) indicate neurons and their corresponding projections in different regions in the female brain. The scale bar shown in (O) is 50 μm. (P) Model for the SP induced post-mating response. SP interferes with interpretation of sensory cues, for example, vision, hearing, smell, taste, and touch at distinct sites in the brain indicated by higher order projections revealed by intersectional expression in the following patterns: SPR8 ∩ dsx (blue), fru11/12 ∩ dsx (black), SPR8 ∩ fru11/12 (yellow), SPR8 ∩ FD6 (pink), and fru11/12 ∩ FD6 (olive). and VNC (fru11/12 ∩ dsx) during higher order neuronal processing.
Figure 12—figure supplement 1. trans-Tango identifies post-synaptic proceeding neurons of sex peptide (SP) targets in the ventral nerve cord (VNC), but not the genital tract.
In the brain, the retro-Tango analysis did not identify primary sensory neurons, but higher order neurons in the central brain in all five split-GAL4 combinations (Figure 12A–E). In addition, neurons in the suboesophageal ganglion were marked from SPR8 intersections with dsx and FD6, and in dsx ∩ fru11/12. In dsx ∩ fru11/12, neurons in the optic lobe (medulla) were marked. In addition, a strong signal was observed in all five split-GAL4 combinations in the mushroom bodies (Figure 12A–E). Although mushroom bodies are dispensable for PMRs (Fleischmann et al., 2001), their connection to SP target neurons indicates an experience-dependent component of PMRs.
The trans-Tango analysis identified a subset of neurons with cell bodies in the suboesophageal ganglion with projections to the pars intercerebralis for SPR8 ∩ dsx and fru11/12 ∩ dsx neurons (Figure 12K and L). For SPR8 ∩ fru11/12 and SPR8 ∩ FD6 neurons, common target neurons were found in the antennal mechanosensory and motor centre (AMMC) region with a single neuron identified near the mushroom body region (Figure 12M and N; Ishimoto and Kamikouchi, 2021). For fru11/12 ∩ FD6, no obvious targets were identified in the central brain (Figure 12O).
In the VNC, the trans-Tango analysis showed post-synaptic targets within the abdominal ganglion with all five split-GAL4 combinations indicating an interconnected neuronal network (Figure 12—figure supplement 1A–O), which needs to be elaborated in detail. In the genital tract, no post-synaptic targets were detected, indicating that these are afferent neurons integrating sensory input (Figure 12—figure supplement 1P–AD).
Taken together, circuitries identified via retro- and trans-Tango place SP target neurons at the interface of sensory processing interneurons connecting to two commonly shared post-synaptic processing neuronal populations in the brain. Hence, our data indicate that SP interferes with sensory input processing from multiple modalities that are canalised to higher order processing centres to generate a behavioural output.
Discussion
Much has been learned about the neuronal circuitry governing reproductive behaviours in Drosophila from interfering with neuronal activity in few neurons selected by intersectional expression using split-GAL4 (Wang et al., 2020a; Wang et al., 2020b; Wang et al., 2021). However, how SP signalling as main inducer of the PMR, prominently consisting of refractoriness to re-mate and induction of egg laying, is integrated in this circuitry is not completely understood (Haussmann et al., 2013).
Here, we addressed this gap by identifying regulatory regions in SPR, fru, and dsx genes driving membrane-tethered expression of SP in subsets of neurons to delineate SP targets to very few neurons in the central brain and the VNC by intersectional expression. Consistent with previous analysis describing multiple pathways for the SP response (Haussmann et al., 2013), we find five distinct populations of interneurons in the central brain directing PMRs. In SP target neurons in the central brain, SPR is essential to induce PMRs when receiving SP from males through mating. From mapping post-synaptic targets by trans-Tango, we identified two populations of interneurons. The architecture of this circuitry is reminiscent of processing of sensory input transmitted to central brain pattern generators for behavioural output. Hence, SP interferes at several levels for coordinating PMRs, but also leaves the female the opportunity to interfere under unfavourable conditions with specific elements of PMRs, for example, if there is no egg laying substrate, females will still not remate (Haussmann et al., 2013). Likewise, mated females will not lay eggs despite suitable egg laying substrates if parasitoid wasps are present (Kacsoh et al., 2015). Thus, the architecture of female PMRs contrasts with male-courtship behaviour consisting of a sequel of behavioural elements that once initiated will always follow stereotypically to the end culminating in mating, or start from the beginning when interrupted (Hall, 1994; Greenspan and Ferveur, 2000).
SP induces PMRs via entering the hemolymph to target neurons in the central brain and ventral nerve cord
Early characterisation of the SP signalling cascade demonstrated induction of PMRs from various other sources than mating, including transgenic secretion from the fat body, expression as membrane-tethered form on neurons or injection of synthetic peptide into the hemolymph (Chen et al., 1988; Aigaki et al., 1991; Schmidt et al., 1993; Nakayama et al., 1997). Likewise, SP is detected in the haemolymph after mating at a PMR inducing concentration (Haussmann et al., 2013). Moreover, PMRs are induced faster when SP is injected compared to induction by mating (Haussmann et al., 2013). This delay, however, is not attributed to sperm binding of SP as it is unchanged after mating with spermless males. These results suggest that SP reaches its targets through entering the circulatory system to target neurons and contrasts a previously proposed model favouring genital tract neurons as SP sensors from the lumen of the genital tract (Häsemeyer et al., 2009; Yang et al., 2009; Rezával et al., 2012). We previously observed binding of radiolabelled SP to various sites in the nervous system including afferent nerves, but these signals likely reflect binding to broadly expressed SPR rather than binding to SPRINz (Ottiger et al., 2000; Ding et al., 2003; Yapici et al., 2008; Haussmann et al., 2013).
In further support of the internalisation model, we identified GAL4 drivers that express mSP in genital tract neurons, but do not induce PMRs. Also, SPR12 does not express in genital tract neurons, but induces egg laying by expression of mSP. Moreover, expression of mSP predominantly in the trunk (including all genital tract sensory neurons) only induces egg laying, but does not change receptivity. Likewise, expression of mSP specifically in the brain (SPR8 ∩ dsx) can reduce receptivity and induce egg laying indistinguishable from mated females.
A ppkGAL4 line generated by P-element mediated transformation can induce PMRs by expression of UAS mSP (Grueber et al., 2003). The same promoter fragment fused to a GAL4 DBD and inserted by phiC31 integration into a landing site intersected with pan-neural nSyb AD line (Seidner et al., 2015; Riabinina et al., 2019), however, does not induce an SP response despite being expressed in genital tract neurons. We found that the ppkGAL4 expresses in a few neurons in the brain and VNC (Nallasivan et al., 2021), but this expression is absent in nSyb ∩ ppk intersection. Likely, the ppkGAL4 construct is inserted in a locus that contains an enhancer that drives expression in SP target neurons.
These results are in strong favour of SP entering the hemolymph to target neurons in the VNC for inducing egg laying, and in the central brain for reducing receptivity and inducing egg laying (Haussmann et al., 2013).
Integration of SP signalling into the circuitry directing reproductive behaviours
Reduction of receptivity and induction of egg laying are both induced by the same critical concentration of injected SP (Schmidt et al., 1993; Haussmann et al., 2013) initially suggesting a simple on/off system for PMRs likely initiated from a small population of neurons. However, such a model would not allow us to split the SP response into individual PMR components by expression of mSP.
Here, we identified several GAL4 drivers, which can induce only egg laying (SPR12, FD3, FD4, and tsh GAL4), but do not reduce receptivity, and others that can only reduce receptivity (oviEN-SS2, oviIN-SS1, and vpoDN-SS1), but do not induce egg laying. Strikingly, tshGAL4, which expresses predominantly in the trunk, only affects egg laying, suggesting a role for the abdominal ganglion in egg laying. Moreover, dsx and all of the SBRINz split-GAL4 combinations affect egg laying and express in the abdominal ganglion (Rezával et al., 2012; Zhou et al., 2014). Hence, this neuronal structure has a key role in regulating egg laying. Since more than a single neuronal population seems to direct egg laying, further high-resolution mapping is required to identify individual neuronal population within the abdominal ganglion (Jang et al., 2017; Oliveira-Ferreira et al., 2023).
Since tshGAL4 only induces egg laying, neurons in the brain must direct reduction of receptivity. Through intersectional expression in combination with head-specific expression of otdflp, we could express mSP only in the brain by FLP-mediated brain-specific excision of a stop cassette. We observed a significant reduction in receptivity for all five intersections tested, but for four, the response is only partial, likely due to the inefficiency of FLP-mediated recombination.
Moreover, brain neurons can also induce egg laying when SPR8 is intersected with dsx, and to some extent also from SPR8 intersection with fru11/12. Due to the inefficiency of FLP-mediated recombination, however, this is likely an underestimate and solving this issue requires development of more robust tools.
In any case, however, our results show that PMRs can be induced from mSP expression from several sites, suggesting interference with processing of sensory information at the level of interneurons. In particular, SPR8 ∩ fru11/12 neurons resemble auditory AMMC-B2 neurons involved in processing of information of the male love song (Yamada et al., 2018). Likewise, SPR8 ∩ dsx neurons seem to overlap with dimorphic dsx pCL2 interneurons that are part of the 26 neurons constituting the pC2 neuronal population involved in courtship song sensing, mating acceptance and ovipositor extrusion for rejection of courting males (Kimura et al., 2015; Deutsch et al., 2019; Wang et al., 2020a). The SPR8 ∩ FD6 neurons resemble dopaminergic fru P1 neurons involved in courtship and the fru11/12 ∩ dsx neurons seem to overlap with dsx pCd and neuropeptide F neurons involved in courtship (Zhang et al., 2021). In females, pC1d neurons have been linked to aggression (Deutsch et al., 2020; Schretter et al., 2020). The fru11/12 ∩ FD6 neurons resemble a class of gustatory pheromone sensing neurons (Sakurai et al., 2013). Although we likely have not identified all SP sensing neurons, our resources will provide a handle to future exploration of the details of this neuronal circuitry incorporating SP signalling for inducing PMRs.
Conclusions
We have identified distinct SP response-inducing neurons (SPRINz) in the central brain and the VNC. Since these five different SP response-inducing neuronal populations in the central brain converge into two target sites, our data suggest a model (Figure 12P), whereby SP signalling interferes with integration of sensory input. Independent interference with different sensory modalities opts for the female to counteract male manipulation at the level of perception of individual sensory cues to adapt to varying physiological and environmental conditions to maximise reproductive success.
Materials and methods
Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | Wild-type: Canton S | This study | RRID:BDSC_64349 | Wild-type strain |
| Genetic reagent (D. melanogaster) | w*; UASmSP (3rd, 61C) | Nakayama et al., 1997 | Gift from T. Aigaki | |
| Genetic reagent (D. melanogaster) | dsx-GAL4 inserted into the endogenous dsx gene (84E5-84E6) | Rideout et al., 2010 | Gift from S. Goodwin | |
| Genetic reagent (D. melanogaster) | fru-GAL4 inserted into the endogenous fru gene (91A6-91B3) | Dickson lab | RRID:BDSC_66870 | |
| Genetic reagent (D. melanogaster) | nSyb GAL4 (3rd) | Rezával et al., 2012 | Gift from S. Goodwin | |
| Genetic reagent (D. melanogaster) | ppk-GAL4/CyO | Bloomington Stock Centre | RRID:BDSC_49021 | |
| Genetic reagent (D. melanogaster) | tshGAL4-1/CyO | Bloomington Stock Centre | RRID:BDSC_3040 | |
| Genetic reagent (D. melanogaster) | elav FRTstopFRT GAL4 | Zaharieva et al., 2015 | ||
| Genetic reagent (D. melanogaster) | otdflp | Asahina et al., 2014 | Gift from D. Anderson | |
| Genetic reagent (D. melanogaster) | UASmCD8GFP (X) | Bloomington Stock Centre | RRID:BDSC_5136 | |
| Genetic reagent (D. melanogaster) | UASmCD8GFP (2nd) | Bloomington Stock Centre | RRID:BDSC_5137 | |
| Genetic reagent (D. melanogaster) | UAS-H2B::YFP (2nd) | Li et al., 2020 | Gift from A. Hidalgo | |
| Genetic reagent (D. melanogaster) | elavLexA (2nd) | Bloomington Stock Centre | RRID:BDSC_52676 | |
| Genetic reagent (D. melanogaster) | LexAop NLStomato (2nd) | Bloomington Stock Centre | RRID:BDSC_66690 | |
| Genetic reagent (D. melanogaster) | UAS TNT (2nd) | Sweeney et al., 1995 | Gift from J.J. Hodge | |
| Genetic reagent (D. melanogaster) | UAS TrpA1 (3rd) | Bloomington Stock Centre | RRID:BDSC_26264 | |
| Genetic reagent (D. melanogaster) | UASFlybow 1.1 (myrGFP, 2nd) | Bloomington Stock Centre | RRID:BDSC_35537 | |
| Genetic reagent (D. melanogaster) | UAS-NaCh::BacGFP (3rd) | Bloomington Stock Centre | RRID:BDSC_9467 | |
| Genetic reagent (D. melanogaster) | UAS Reaper/FM7;UAS Hid/CyO | Bloomington Stock Centre | RRID:BDSC_5823 | |
| Genetic reagent (D. melanogaster) | UAS FRTstopFRT GFP/CyO | Dickson lab | RRID:BDSC_30125 | |
| Genetic reagent (D. melanogaster) | UAS FRTstopFRT TNT/CyO | Dickson lab | RRID:BDSC_30125 | |
| Genetic reagent (D. melanogaster) | UAS FRTstopFRT TrpA1/CyO | Dickson lab | RRID:BDSC_30125 | |
| Genetic reagent (D. melanogaster) | UAS FRTstopFRT mSP (3rd) | This study | Soller Lab | UAS mSP line with a stop cassette |
| Genetic reagent (D. melanogaster) | UAS dicer2; UAS SPR RNAi (X, 3rd) | Yapici et al., 2008 | Gift from B. Dickson lab | |
| Genetic reagent (D. melanogaster) | SPR | Bloomington Stock Centre | RRID:BDSC_7708 | |
| Genetic reagent (D. melanogaster) | Df(1)JC70/FM7c | Bloomington Stock Centre | RRID:BDSC_944 | |
| Genetic reagent (D. melanogaster) | nSyb p65-GAL4.AD (attP40) | Riabinina et al., 2019 | Gift from O. Riabinina | |
| Genetic reagent (D. melanogaster) | SPR8 AD: VT057286-p65.AD (attP40) | Bloomington Stock Centre | RRID:BDSC_71392 | |
| Genetic reagent (D. melanogaster) | Fru11/12 AD: VT043695-p65.AD (attP40) | Bloomington Stock Centre | RRID:BDSC_72065 | |
| Genetic reagent (D. melanogaster) | dsx DBD | Rideout et al., 2010 | Gift from S. Goodwin | |
| Genetic reagent (D. melanogaster) | dsx24 DBD: R42G02-GAL4.DBD (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR8 DBD: VT057286-Gal4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_71425 | |
| Genetic reagent (D. melanogaster) | fru11/12 DBD: VT043695-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_72788 | |
| Genetic reagent (D. melanogaster) | FD6 DBD: VT003280-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_75877 | |
| Genetic reagent (D. melanogaster) | ppk DBD: ppk-GAL4.DBD (VK00027, 89E11) | Seidner et al., 2015 | Gift from W. J. Joiner | |
| Genetic reagent (D. melanogaster) | SPR12 AD: VT057292-p65.AD (attP40) | Bloomington Stock Centre | RRID:BDSC_72924 | |
| Genetic reagent (D. melanogaster) | UAS-myrGFP QUAS-mtdTomato-3xHA; trans-Tango | Bloomington Stock Centre | RRID:BDSC_95317 | |
| Genetic reagent (D. melanogaster) | QUAS-mtdTomato-3xHA; retro-Tango | Sorkaç et al., 2023 | RRID:BDSC_99661 | Gift from G. Barnea |
| Genetic reagent (D. melanogaster) | fru1 GAL4: R23C03-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49021 | |
| Genetic reagent (D. melanogaster) | fru2 GAL4, R22H11-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_48043 | |
| Genetic reagent (D. melanogaster) | fru3 GAL4, R21H09-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49867 | |
| Genetic reagent (D. melanogaster) | fru4 GAL4, R23C12-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49026 | |
| Genetic reagent (D. melanogaster) | fru5 GAL4, R22F06-GAL4 (attP2) | Korea Drosophila Resource Center | KDRC 11848 | |
| Genetic reagent (D. melanogaster) | fru6 GAL4, R23D03GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | fru7 GAL4, R22B09-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | fru8 GAL4, R23B12-GAL4 (attP2) | Korea Drosophila Resource Centre | KDRC 11849 | |
| Genetic reagent (D. melanogaster) | fru9 GAL4, R22A02-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49868 | |
| Genetic reagent (D. melanogaster) | fru10 GAL4, R22C05-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49301 | |
| Genetic reagent (D. melanogaster) | fru11 GAL4, R22C11-lexA (attP40) | Bloomington Stock Centre | RRID:BDSC_52604 | |
| Genetic reagent (D. melanogaster) | fru12 GAL4, R22A11-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_48966 | |
| Genetic reagent (D. melanogaster) | fru13 GAL4, R23A06-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49009 | |
| Genetic reagent (D. melanogaster) | fru14 GAL4, R22C03-GAL4 (attP2) | Korea Drosophila Resource Centre | RRID:KDRC_11868 | |
| Genetic reagent (D. melanogaster) | fru15 GAL4, R23B04-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49016 | |
| Genetic reagent (D. melanogaster) | fru16 GAL4, R22C07-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_48975 | |
| Genetic reagent (D. melanogaster) | fru17 GAL4, R23C08-GAL4 (attP2) | Korea Drosophila Resource Centre | KDRC 11835 | |
| Genetic reagent (D. melanogaster) | fru18 GAL4, R23C07GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | fru19 GAL4, R22B10-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_48969 | |
| Genetic reagent (D. melanogaster) | fru20 GAL4, R22E10-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49302 | |
| Genetic reagent (D. melanogaster) | fru21 GAL4, R22D11-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_48982 | |
| Genetic reagent (D. melanogaster) | fru22 GAL4, R22H07-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_490003 | |
| Genetic reagent (D. melanogaster) | fru23 GAL4, R21H02-GAL4 (attP2) | Korea Drosophila Resource Centre | KDRC 11847 | |
| Genetic reagent (D. melanogaster) | fru24 GAL4, R23B11-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_49019 | |
| Genetic reagent (D. melanogaster) | fru25 GAL4: VT043674-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | fru26 GAL4, VT043675-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | fru27 GAL4, VT043676-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx1 GAL4, R39E06-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_50051 | |
| Genetic reagent (D. melanogaster) | dsx2 GAL4, R40A05-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_48138 | |
| Genetic reagent (D. melanogaster) | dsx3 GAL4, R40F03-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_47355 | |
| Genetic reagent (D. melanogaster) | dsx4 GAL4, R40F04-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx5 GAL4, R41A01-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx6 GAL4, R41D01GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx7 GAL4, R41F06-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_47584 | |
| genetic reagent (D. melanogaster) | dsx8 GAL4, R42C06-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_50150 | |
| Genetic reagent (D. melanogaster) | dsx9 GAL4, R42D02-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_41250 | |
| Genetic reagent (D. melanogaster) | dsx10 GAL4, R42D04-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_47588 | |
| Genetic reagent (D. melanogaster) | dsx11 GAL4, VT038171-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx12 GAL4, VT038169-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx13 GAL4, VT038167-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx14 GAL4, VT038166-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx15 GAL4, VT038161-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx16 GAL4, VT038159-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx17 GAL4, VT038157-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx18 GAL4, VT038155-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx19 GAL4, VT038151-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx20 GAL4, VT038149-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| genetic reagent (D. melanogaster) | dsx21 GAL4, P{VT038148-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx22 GAL4, P{VT038147-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| genetic reagent (D. melanogaster) | dsx23 GAL4, R22H07-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx24 GAL4, R21H02-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | dsx25 GAL4, R21B01-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR1 GAL4, R78F09-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR2 GAL4, R78F11-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR3 GAL4, R78E11-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR4 GAL4, R78E12-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40002 | |
| Genetic reagent (D. melanogaster) | SPR5 GAL4, R78G09-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40015 | |
| Genetic reagent (D. melanogaster) | SPR6 GAL4, R78G08-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR7 GAL4, R78F07-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_47409 | |
| genetic reagent (D. melanogaster) | SPR8 GAL4, R78F10-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40007 | |
| Genetic reagent (D. melanogaster) | SPR9 GAL4, R78G02-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40010 | |
| Genetic reagent (D. melanogaster) | SPR10 GAL4, R78G07-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR11 GAL4, R78G04-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40012 | |
| Genetic reagent (D. melanogaster) | SPR12 GAL4, R78F05-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR13 GAL4, R78G05-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_41308 | |
| Genetic reagent (D. melanogaster) | SPR14 GAL4, R78G06-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR15 GAL4, R78G03-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40011 | |
| Genetic reagent (D. melanogaster) | SPR16 GAL4, R78F06-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR17 GAL4, R78F12-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR18 GAL4, R78F03-GAL4 | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR19 GAL4, R78F01-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40003 | |
| Genetic reagent (D. melanogaster) | SPR20 GAL4, R78G01-GAL4 (attP2) | Bloomington Stock Centre | RRID:BDSC_40009 | |
| Genetic reagent (D. melanogaster) | SPR21 GAL4, R78F02-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | SPR22 GAL4, R78F08-GAL4 (attP2) | Bloomington Stock Centre | N/A | |
| Genetic reagent (D. melanogaster) | FD1 GAL4, VT050405-GAL4 (attP2) | Vienna Drosophila Stock Centre | VDSC | |
| Genetic reagent (D. melanogaster) | FD2 GAL4, VT007068-GAL4 (attP2) | Vienna Drosophila Stock Centre | VDSC | |
| Genetic reagent (D. melanogaster) | FD3 GAL4, VT045154-GAL4 (attP2), | Vienna Drosophila Stock Centre | VDSC | |
| Genetic reagent (D. melanogaster) | FD4 GAL4, VT000454-GAL4 (attP2) | Vienna Drosophila Stock Centre | VDSC | |
| Genetic reagent (D. melanogaster) | FD5 GAL4, VT050247-GAL4 (attP2) | Vienna Drosophila Stock Centre | VDSC | |
| Genetic reagent (D. melanogaster) | FD6 GAL4, VT003280-GAL4 (attP2) | Vienna Drosophila Stock Centre | VDSC | |
| Genetic reagent (D. melanogaster) | SPR8 GAL4, R78F10-GAL4 (attP2); | Bloomington Stock Centre | RRID:BDSC_40007 | |
| Genetic reagent (D. melanogaster) | SPR8 AD [VT057286-p65.AD (attP40)]; fru11/12 DBD [VT043695-GAL4.DBD (attP2)] | This study | Soller Lab | Split gal4 combination of SPR8-AD and fru11/12-DBD |
| Genetic reagent (D. melanogaster) | Fru11/12 AD [VT043695-p65.AD (attP40)]; FD6 DBD [VT003280-GAL4.DBD (attP2)] | This study | Soller Lab | Split gal4 combination of fru11/12-AD and FD6-DBD |
| Genetic reagent (D. melanogaster) | SPR8 AD [VT057286-p65.AD (attP40)]; FD6 DBD [VT003280-GAL4.DBD (attP2)] | This study | Soller Lab | Split gal4 combination of SPR8-AD and FD6-DBD |
| Genetic reagent (D. melanogaster) | SPR8 AD [VT057286-p65.AD (attP40)]; dsx DBD (attP2) | This study | Soller Lab | Split gal4 combination of SPR8-AD and dsx-DBD |
| Genetic reagent (D. melanogaster) | Fru11/12 AD [VT043695-p65.AD (attP40)]; dsx DBD (attP2) | This study | Soller Lab | Split gal4 combination of fru11/12-AD and dsx-DBD |
| Genetic reagent (D. melanogaster) | VT058873-GAL4.AD (attP40); SPR8 DBD [VT057286-GAL4.DBD(attP2)] | This study | Soller Lab | Split gal4 combination of SPSN-AD and SPR8-DBD |
| Genetic reagent (D. melanogaster) | VT058873-GAL4.AD (attP40); Fru11/12 DBD [VT043696-GAL4.DBD(attP2)] | This study | Soller Lab | Split gal4 combination of SPSN-AD and fru11/12-DBD |
| Genetic reagent (D. melanogaster) | VT058873-GAL4.AD (attP40); dsx DBD (attp2) | This study | Soller Lab | Split gal4 combination of SPSN-AD and dsx-DBD |
| Genetic reagent (D. melanogaster) | nSyb p65-GAL4.AD (attp40); ppk DBD: ppk-GAL4.DBD [VK00027, 89E11] | This study | Soller Lab | Split gal4 combination of nSYB-AD and ppk-DBD |
| Genetic reagent (D. melanogaster) | SAG1, VT050405-GAL4.AD (attP40); VT007068-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_66875 | Split gal4 combination of SAG1-AD and SPSN-DBD |
| Genetic reagent (D. melanogaster) | pC1-SS1, VT2002064-GAL4.AD (attP40); VT008469-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86830 | |
| Genetic reagent (D. melanogaster) | oviDN-SS1, VT050660-GAL4.AD (attP40); VT028160-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86832 | |
| Genetic reagent (D. melanogaster) | oviDN-SS2, VT026873-GAL4.AD (attP40); VT040574-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86831 | |
| Genetic reagent (D. melanogaster) | oviEN-SS1, VT043086-GAL4.AD (attP40); VT034612-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86839 | |
| Genetic reagent (D. melanogaster) | oviEN-SS2, VT034612-GAL4.AD (attP40); VT050229-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86833 | |
| Genetic reagent (D. melanogaster) | oviIN-SS1, R68A10-GAL4.AD (attP40); VT010054-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86837 | |
| Genetic reagent (D. melanogaster) | oviIN-SS2, VT026347-GAL4.AD (attP40); VT026035-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86838 | |
| Genetic reagent (D. melanogaster) | vpoDN-SS1, R31D07-GAL4.AD (attP40); R52F12-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86868 | |
| Genetic reagent (D. melanogaster) | SPSN1, VT058873-GAL4.AD (attP40); VT003280-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86834 | |
| Genetic reagent (D. melanogaster) | SPSN2, VT058873-GAL4.AD (attP40); VT033490-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_86870 | |
| Genetic reagent (D. melanogaster) | SAG1, VT050405-GAL4.AD (attP40); VT007068-GAL4.DBD (attP2) | Bloomington Stock Centre | RRID:BDSC_66875 | |
| Strain, strain background (Escherichia coli) | DH5α | New England Biolabs | RRID:AB_10015282 | For recombinant DNA cloning: |
| Antibody | Anti-HA (rat monoclonal antibody, clone 3F10) | Roche | RRID:AB_390919 | 1:20 |
| Antibody | Anti-GFP (rabbit Polyclonal Antibody) | Molecular Probes | RRID:AB_221570 | 1:100 |
| Antibody | Goat anti-rabbit Alexa Fluor 488 (goat polyclonal antibody) |
Molecular Probes | RRID:AB_143165 | 1:250 |
| Antibody | Goat anti-rabbit Alexa Fluor 546 (goat polyclonal antibody) |
Molecular Probes | RRID:AB_2534077 | 1:250 |
| Antibody | Goat anti-rabbit Alexa Fluor 647 (goat polyclonal antibody) |
Molecular Probes | RRID:AB_2535813 | 1:250 |
| Antibody | Goat anti-rat Alexa Fluor 647 (goat polyclonal antibody) |
Molecular Probes | RRID:AB_141778 | 1:250 |
| Sequence-based reagent | pUAST-GGTmSP FRTGFPstopFRT gBlock (FRT underlined) | IDT | Soller Lab |
GAATTGGGAATTCGTTAACAGATCTGCGATCG
CGGCCCGGGGATCTTGAAGTTCCTATTCCGAAG TTCCTATTCTCTAGAAAGTATAGGAACTTCAGAGCGCTTTTGAAGCTAGCTAAAGAGCCTGCTAAAGCAAAAAAGAAGTCACCATGGTGTCGAGCGCAAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGA GGGCGAGGGCGATGCCACCTACGGCAAGCTG ACCCTGAAGTTCATCTGCACCACCGGCAAGCT GCCCGTGCCCTGGCCCACCCTCGTGACCACC CTGACCTACGGCGTGCAGTGCTTCAGCCGCTA CCCCGACCACATGAAGCAGCACGACTTCTTCA AGTCCGCCATGCCCGAAGGCTACGTCCAGGAG CGCACCATCTTCTTCAAGGACGACGGCAACTA CAAGACCCGCGCCGAGGTGAAGTTCGAGGGC GACACCCTGGTGAACCGCATCGAGCTGAAGGG CATCGACTTCAAGGAGGACGGCAACATCCTGG GGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTACTCAGATCTTTGCAAGCTTGTAGAGTTTCCCATTTAATAATTCATATTATCTCGAATCTAGTCAATTACGGCTTTCCTCAAATAGAAAAATAAAAAAAATGAAAAAATGCACTTGCCATTTAAACTTAGACGCGATAACGAATTC CGGGGATCTTGAAGT TCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGA A CTTCAGAGCGCTTTTGAAGCTGCGGCCGCGGCTC G A CGGTATCGATAAGCTTG |
| Software, algorithm | GraphPad Prism | GraphPad Prism | RRID:SCR_002798 | Software |
| Software, algorithm | Fiji | Fiji | RRID:SCR_002285 | Software |
Fly strains and husbandry
Flies were kept on standard cornmeal-agar food (1% industrial-grade agar, 2.1% dried yeast, 8.6% dextrose, 9.7% cornmeal, and 0.25% Nipagin, all in [w/v]) in a 12-hour light: 12-hour dark cycle. Propionic acid was omitted from fly food as acidity affects egg laying (Gou et al., 2014). Genetic crosses were done in vials and kept at low density to ensure larvae were not competing for food and if necessary, additional live yeast was added. For all behavioural assays, virgin and mated Canton-S were used as controls. Virgin females, for example, from crosses of GAL4 with UASmSP, were collected after emergence within a 5-hour window and well-fed with live yeast sprinkled on food for maximum egg production and allowed to sexually mature (3–5 days).
To recombine second chromosome inserts for split-GAL4AD (attP40) and third chromosome split-GAL4DBD (attP2), standard genetic crossing schemes were used and final stocks were balanced with CyO and TM3 Sb (combined from ST and CT stock, see Key Resources Table). Split-Gal4AD and DBD combination lines were then crossed to UASmSP. For meiotic recombination, final stocks were validated by behavioural analysis for UAS mSP, for flp with eFeG UASCD8GFP to monitor GFP expression and for otdflp UASstopTrpA and otdflp UASstopTNT by crossing to elavGAL4 and monitored by lethality.
For enhanced recombination with flp, virgin females were transferred to 30°C after eclosion and kept for 5 days at this temperature before performing the behavioural assays. For induction of neuronal activity by temperature-sensitive TrpA1, females were kept at 30°C.
To make UAS FRTstopFRT mSP, a gBlock (IDT) stop cassette with the FRT sequences used in the eFeG plasmid (Haussmann et al., 2008) was inserted into NotI cut pUAST-GGTmSP (gift from T. Aigaki) by Gibson assembly. In the stop-cassette, the FRT sequence is followed by a GFP with a 3’UTR from ewg containing polyA site 1 from intron 6 (Haussmann et al., 2011). Flies were transformed by P-element-mediated transgenesis and inserts on each chromosome were established that show a robust PMR with dsxflp indistinguishable from mated females.
Behavioural analysis
Females were examined for the main post-mating behaviours receptivity and oviposition as described previously and as follows (Soller et al., 1999; Soller et al., 2006). To generate mated females, one female and three males were added to fly vials and observed until mating and males were removed after mating. For receptivity tests, mature 3–7-day-old virgin or mated females were added to fly vials (95 mm length and 24 mm diameter) containing Canton S males with an aspirator and observed for 1 hour, generally three females and seven males. For these experiments, males were separated from females at least 1 day before the experiment. Receptivity tests were done in the afternoon with virgins, or 5–24 hours after mating for controls. For oviposition, females were placed individually in fly vials in the afternoon and the number of eggs laid was counted the next day. Receptivity and oviposition tests were tested were done blinded.
Statistical analysis
Sample size was based on previous studies, non-blinded and not predetermined by statistical methods (Soller et al., 1997; Haussmann et al., 2013; Nallasivan et al., 2021). Behavioural data are representatives of at least three replicates that were performed on three different days. Statistical analysis of behavioural experiments was performed using GraphPad Prism 9 (GraphPad by Dotmatics, RRID:SCR_002798) using one-way ANOVA followed by pairwise comparisons with Tukey’s test.
Immunohistochemistry and imaging
For the analysis of adult neuronal projection from UAS CD8GFP, UAS H2BYFP,UASmyrGFP, lexAopNLStomato, or QUAS mtdtomato3xHA expressing brains, VNCs or genital tracts, tissues were dissected in PBS (137 mM NaCl, 10 mM phosphate, 2.7 mM KCl, pH 7.4), fixed in 4% (w/v in PBS) paraformaldehyde for 15 minutes, washed three times in PBST (PBS with 1% BSA and 0.3% Triton-X100), then once in PBS for 10 minutes, mounted in Vectashield (Vector Labs) and visualised with confocal microscopy using a Leica TCS SP8. If signals were weak, antibody in-situ stainings were done as described previously (Haussmann et al., 2008) for validation using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-GFP (Molecular Probes, 1:100) and visualised with Alexa Fluor 488 (1:250; Molecular Probes or Invitrogen), Alexa Fluor 546 (1:250; Molecular Probes or Invitrogen), or Alexa Fluor 647 (1:250; Molecular Probes or Invitrogen). For imaging, tissues were mounted in Vectashield (Vector Labs).
Confocal microscopy and image processing
Adult tissues were scanned using a Leica SP8 confocal microscope equipped with a set of fluorescent filters and hybrid detector (HyD). Adult brains were scanned using a 40× HC PL APO 40×/1.30 lens with oil, 1024 × 1024 resolution and 0.96 µm Z-step. VNC and genital tracts were scanned using a HC PL APO CS2 20×/0.75 with oil, 1024 × 1024 resolution and 0.96 µm Z-step. Images were obtained using Leica Application Suite X (LAS X) imaging acquisition software. Raw data files were in LIF format and were processed using FIJI RRID:SCR_002285.
For high-resolution mapping, neurons were identified in the virtual fly brain based on registered GAL4 expression and traces retrieved for modelling (Scheffer et al., 2020; Phelps et al., 2021; Galili et al., 2022).
Acknowledgements
We thank T Aigaki, G Barnea, P Soba, WJ Joiner, B Dickson, S Goodwin, C Rezaval, D Anderson, JJ Hodge, A Hidalgo, S Collier, O Raibinina, the Bloomington Stock Centre, the Vienna Drosophila RNAi Center for flies, T Aigaki and WJ Joiner for plasmids, the University of Cambridge Department of Genetics Fly Facility and FlyORF for injections, D Scocchia for help with PCR, and IU Haussmann, YJ Kim, JC Billeter, and J-R Martin for comments on the manuscript. We acknowledge funding by the Biotechnology and Biological Science Research Council (BB/N021827/1 and BB/Y006364/1) to MS.
Funding Statement
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Contributor Information
Matthias Soller, Email: matthias.soller@manchester.ac.uk.
Ilona C Grunwald Kadow, University of Bonn, Germany.
Albert Cardona, University of Cambridge, United Kingdom.
Funding Information
This paper was supported by the following grants:
Biotechnology and Biological Sciences Research Council BB/Y006364/1 to Matthias Soller.
Biotechnology and Biological Sciences Research Council BB/N021827/1 to Matthias Soller.
Additional information
Competing interests
No competing interests declared.
Author contributions
Conceptualization, Resources, Formal analysis, Investigation, Visualization, Methodology, Performed genetic experiments and imaging.
Resources, Data curation, Formal analysis, Investigation, Performed genetic experiments and imaging.
Formal analysis, Investigation, Performed genetic experiments.
Conceptualization, Resources, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing – original draft, Project administration, Writing – review and editing, Designed and performed genetic experiments and analyzed data, Wrote the manuscript with support from MPN.
Ethics
Ethical review and approval was not required for this study because this study was conducted with an invertebrate model – fruit flies (Drosophila melanogaster). Experiments with invertebrates are not regulated by law.
Additional files
Data availability
Brain and VNC images for splitGal4 combinations of SP Response Inducing Neurons have been deposited in Virtual Fly Brain and will be published under the following accession numbers: VFB_x0000000-9. All data generated or analysed during this study are included in the paper and supplementary files; source data files are provided for all figures.
The following dataset was generated:
Nallasivan MP, Singh DND, Saleh MSRS, Soller M. 2025. Sex Peptide Response Inducing Neurons (SPRINz) Virtual Fly Brain. VFB_x0000000-9
References
- Aigaki T, Fleischmann I, Chen PS, Kubli E. Ectopic expression of sex peptide alters reproductive behavior of female D. melanogaster. Neuron. 1991;7:557–563. doi: 10.1016/0896-6273(91)90368-a. [DOI] [PubMed] [Google Scholar]
- Anderson DJ. Circuit modules linking internal states and social behaviour in flies and mice. Nature Reviews. Neuroscience. 2016;17:692–704. doi: 10.1038/nrn.2016.125. [DOI] [PubMed] [Google Scholar]
- Aranha MM, Vasconcelos ML. Deciphering Drosophila female innate behaviors. Current Opinion in Neurobiology. 2018;52:139–148. doi: 10.1016/j.conb.2018.06.005. [DOI] [PubMed] [Google Scholar]
- Asahina K, Watanabe K, Duistermars BJ, Hoopfer E, González CR, Eyjólfsdóttir EA, Perona P, Anderson DJ. Tachykinin-expressing neurons control male-specific aggressive arousal in Drosophila. Cell. 2014;156:221–235. doi: 10.1016/j.cell.2013.11.045. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Avila FW, Ravi Ram K, Bloch Qazi MC, Wolfner MF. Sex peptide is required for the efficient release of stored sperm in mated Drosophila females. Genetics. 2010;186:595–600. doi: 10.1534/genetics.110.119735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Avila FW, Sirot LK, LaFlamme BA, Rubinstein CD, Wolfner MF. Insect seminal fluid proteins: identification and function. Annual Review of Entomology. 2011;56:21–40. doi: 10.1146/annurev-ento-120709-144823. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Billeter JC, Rideout EJ, Dornan AJ, Goodwin SF. Control of male sexual behavior in Drosophila by the sex determination pathway. Current Biology. 2006;16:R766–R776. doi: 10.1016/j.cub.2006.08.025. [DOI] [PubMed] [Google Scholar]
- Carvalho GB, Kapahi P, Anderson DJ, Benzer S. Allocrine modulation of feeding behavior by the Sex Peptide of Drosophila. Current Biology. 2006;16:692–696. doi: 10.1016/j.cub.2006.02.064. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen PS, Stumm-Zollinger E, Aigaki T, Balmer J, Bienz M, Böhlen P. A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster. Cell. 1988;54:291–298. doi: 10.1016/0092-8674(88)90192-4. [DOI] [PubMed] [Google Scholar]
- Cognigni P, Bailey AP, Miguel-Aliaga I. Enteric neurons and systemic signals couple nutritional and reproductive status with intestinal homeostasis. Cell Metabolism. 2011;13:92–104. doi: 10.1016/j.cmet.2010.12.010. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cury KM, Axel R. Flexible neural control of transition points within the egg-laying behavioral sequence in Drosophila. Nature Neuroscience. 2023;26:1054–1067. doi: 10.1038/s41593-023-01332-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demir E, Dickson BJ. fruitless splicing specifies male courtship behavior in Drosophila. Cell. 2005;121:785–794. doi: 10.1016/j.cell.2005.04.027. [DOI] [PubMed] [Google Scholar]
- Deutsch D, Clemens J, Thiberge SY, Guan G, Murthy M. Shared song detector neurons in Drosophila male and female brains drive sex-specific behaviors. Current Biology. 2019;29:3200–3215. doi: 10.1016/j.cub.2019.08.008. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deutsch D, Pacheco D, Encarnacion-Rivera L, Pereira T, Fathy R, Clemens J, Girardin C, Calhoun A, Ireland E, Burke A, Dorkenwald S, McKellar C, Macrina T, Lu R, Lee K, Kemnitz N, Ih D, Castro M, Halageri A, Jordan C, Silversmith W, Wu J, Seung HS, Murthy M. The neural basis for a persistent internal state in Drosophila females. eLife. 2020;9:e59502. doi: 10.7554/eLife.59502. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ding Z, Haussmann I, Ottiger M, Kubli E. Sex-peptides bind to two molecularly different targets in Drosophila melanogaster females. Journal of Neurobiology. 2003;55:372–384. doi: 10.1002/neu.10218. [DOI] [PubMed] [Google Scholar]
- Domanitskaya EV, Liu H, Chen S, Kubli E. The hydroxyproline motif of male sex peptide elicits the innate immune response in Drosophila females. The FEBS Journal. 2007;274:5659–5668. doi: 10.1111/j.1742-4658.2007.06088.x. [DOI] [PubMed] [Google Scholar]
- Dulac C, Kimchi T. Neural mechanisms underlying sex-specific behaviors in vertebrates. Current Opinion in Neurobiology. 2007;17:675–683. doi: 10.1016/j.conb.2008.01.009. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feng K, Palfreyman MT, Häsemeyer M, Talsma A, Dickson BJ. Ascending SAG neurons control sexual receptivity of Drosophila females. Neuron. 2014;83:135–148. doi: 10.1016/j.neuron.2014.05.017. [DOI] [PubMed] [Google Scholar]
- Fleischmann I, Cotton B, Choffat Y, Spengler M, Kubli E. Mushroom bodies and post-mating behaviors of Drosophila melanogaster females. Journal of Neurogenetics. 2001;15:117–144. doi: 10.3109/01677060109066198. [DOI] [PubMed] [Google Scholar]
- Galili DS, Jefferis GS, Costa M. Connectomics and the neural basis of behaviour. Current Opinion in Insect Science. 2022;54:100968. doi: 10.1016/j.cois.2022.100968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gou B, Liu Y, Guntur AR, Stern U, Yang CH. Mechanosensitive neurons on the internal reproductive tract contribute to egg-laying-induced acetic acid attraction in Drosophila. Cell Reports. 2014;9:522–530. doi: 10.1016/j.celrep.2014.09.033. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greenspan RJ, Ferveur JF. Courtship in Drosophila. Annual Review of Genetics. 2000;34:205–232. doi: 10.1146/annurev.genet.34.1.205. [DOI] [PubMed] [Google Scholar]
- Grueber WB, Ye B, Moore AW, Jan LY, Jan YN. Dendrites of distinct classes of Drosophila sensory neurons show different capacities for homotypic repulsion. Current Biology. 2003;13:618–626. doi: 10.1016/s0960-9822(03)00207-0. [DOI] [PubMed] [Google Scholar]
- Hall JC. The mating of a fly. Science. 1994;264:1702–1714. doi: 10.1126/science.8209251. [DOI] [PubMed] [Google Scholar]
- Häsemeyer M, Yapici N, Heberlein U, Dickson BJ. Sensory neurons in the Drosophila genital tract regulate female reproductive behavior. Neuron. 2009;61:511–518. doi: 10.1016/j.neuron.2009.01.009. [DOI] [PubMed] [Google Scholar]
- Haussmann IU, White K, Soller M. Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways. Genome Biology. 2008;9:R73. doi: 10.1186/gb-2008-9-4-r73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haussmann IU, Li M, Soller M. ELAV-mediated 3’-end processing of ewg transcripts is evolutionarily conserved despite sequence degeneration of the ELAV-binding site. Genetics. 2011;189:97–107. doi: 10.1534/genetics.111.131383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haussmann IU, Hemani Y, Wijesekera T, Dauwalder B, Soller M. Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours. Proceedings. Biological Sciences. 2013;280:20131938. doi: 10.1098/rspb.2013.1938. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hopkins BR, Perry JC. The evolution of sex peptide: sexual conflict, cooperation, and coevolution. Biological Reviews of the Cambridge Philosophical Society. 2022;97:1426–1448. doi: 10.1111/brv.12849. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isaac RE, Li C, Leedale AE, Shirras AD. Drosophila male sex peptide inhibits siesta sleep and promotes locomotor activity in the post-mated female. Proceedings. Biological Sciences. 2010;277:65–70. doi: 10.1098/rspb.2009.1236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishimoto H, Kamikouchi A. Molecular and neural mechanisms regulating sexual motivation of virgin female Drosophila. Cellular and Molecular Life Sciences. 2021;78:4805–4819. doi: 10.1007/s00018-021-03820-y. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jang YH, Chae HS, Kim YJ. Female-specific myoinhibitory peptide neurons regulate mating receptivity in Drosophila melanogaster. Nature Communications. 2017;8:1630. doi: 10.1038/s41467-017-01794-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jenett A, Rubin GM, Ngo T-TB, Shepherd D, Murphy C, Dionne H, Pfeiffer BD, Cavallaro A, Hall D, Jeter J, Iyer N, Fetter D, Hausenfluck JH, Peng H, Trautman ET, Svirskas RR, Myers EW, Iwinski ZR, Aso Y, DePasquale GM, Enos A, Hulamm P, Lam SCB, Li H-H, Laverty TR, Long F, Qu L, Murphy SD, Rokicki K, Safford T, Shaw K, Simpson JH, Sowell A, Tae S, Yu Y, Zugates CT. A GAL4-driver line resource for Drosophila neurobiology. Cell Reports. 2012;2:991–1001. doi: 10.1016/j.celrep.2012.09.011. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kacsoh BZ, Bozler J, Ramaswami M, Bosco G. Social communication of predator-induced changes in Drosophila behavior and germ line physiology. eLife. 2015;4:e07423. doi: 10.7554/eLife.07423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kim YJ, Bartalska K, Audsley N, Yamanaka N, Yapici N, Lee JY, Kim YC, Markovic M, Isaac E, Tanaka Y, Dickson BJ. MIPs are ancestral ligands for the sex peptide receptor. PNAS. 2010;107:6520–6525. doi: 10.1073/pnas.0914764107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kim S-J, Lee K-M, Park SH, Yang T, Song I, Rai F, Hoshino R, Yun M, Zhang C, Kim J-I, Lee S, Suh GSB, Niwa R, Park Z-Y, Kim Y-J. A sexually transmitted sugar orchestrates reproductive responses to nutritional stress. Nature Communications. 2024;15:8477. doi: 10.1038/s41467-024-52807-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kimura K, Sato C, Koganezawa M, Yamamoto D. Drosophila ovipositor extension in mating behavior and egg deposition involves distinct sets of brain interneurons. PLOS ONE. 2015;10:e0126445. doi: 10.1371/journal.pone.0126445. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kubli E. The sex-peptide. BioEssays. 1992;14:779–784. doi: 10.1002/bies.950141111. [DOI] [PubMed] [Google Scholar]
- Kvitsiani D, Dickson BJ. Shared neural circuitry for female and male sexual behaviours in Drosophila. Current Biology. 2006;16:R355–R356. doi: 10.1016/j.cub.2006.04.025. [DOI] [PubMed] [Google Scholar]
- Kvon EZ, Kazmar T, Stampfel G, Yáñez-Cuna JO, Pagani M, Schernhuber K, Dickson BJ, Stark A. Genome-scale functional characterization of Drosophila developmental enhancers in vivo. Nature. 2014;512:91–95. doi: 10.1038/nature13395. [DOI] [PubMed] [Google Scholar]
- Li G, Forero MG, Wentzell JS, Durmus I, Wolf R, Anthoney NC, Parker M, Jiang R, Hasenauer J, Strausfeld NJ, Heisenberg M, Hidalgo A. A Toll-receptor map underlies structural brain plasticity. eLife. 2020;9:e52743. doi: 10.7554/eLife.52743. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li H, Janssens J, De Waegeneer M, Kolluru SS, Davie K, Gardeux V, Saelens W, David F, Brbić M, Leskovec J, McLaughlin CN, Xie Q, Jones RC, Brueckner K, Shim J, Tattikota SG, Schnorrer F, Rust K, Nystul TG, Carvalho-Santos Z, Ribeiro C, Pal S, Przytycka TM, Allen AM, Goodwin SF, Berry CW, Fuller MT, White-Cooper H, Matunis EL, DiNardo S, Galenza A, O’Brien LE, Dow JAT, Jasper H, Oliver B, Perrimon N, Deplancke B, Quake SR, Luo L, Aerts S, FCA Consortium Fly Cell atlas: a single-cell transcriptomic atlas of the adult fruit fly. Genomics. 2022;375:eabk2432. doi: 10.1101/2021.07.04.451050. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luan H, Peabody NC, Vinson CR, White BH. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Neuron. 2006;52:425–436. doi: 10.1016/j.neuron.2006.08.028. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manning A. The control of sexual receptivity in female Drosophila. Animal Behaviour. 1967;15:239–250. doi: 10.1016/0003-3472(67)90006-1. [DOI] [PubMed] [Google Scholar]
- Manoli DS, Foss M, Villella A, Taylor BJ, Hall JC, Baker BS. Male-specific fruitless specifies the neural substrates of Drosophila courtship behaviour. Nature. 2005;436:395–400. doi: 10.1038/nature03859. [DOI] [PubMed] [Google Scholar]
- Nakayama S, Kaiser K, Aigaki T. Ectopic expression of sex-peptide in a variety of tissues in Drosophila females using the P[GAL4] enhancer-trap system. Molecular & General Genetics. 1997;254:449–455. doi: 10.1007/s004380050438. [DOI] [PubMed] [Google Scholar]
- Nallasivan MP, Haussmann IU, Civetta A, Soller M. Channel nuclear pore protein 54 directs sexual differentiation and neuronal wiring of female reproductive behaviors in Drosophila. BMC Biology. 2021;19:226. doi: 10.1186/s12915-021-01154-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nojima T, Rings A, Allen AM, Otto N, Verschut TA, Billeter JC, Neville MC, Goodwin SF. A sex-specific switch between visual and olfactory inputs underlies adaptive sex differences in behavior. Current Biology. 2021;31:1175–1191. doi: 10.1016/j.cub.2020.12.047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oliveira-Ferreira C, Gaspar M, Vasconcelos ML. Neuronal substrates of egg-laying behaviour at the abdominal ganglion of Drosophila melanogaster. Scientific Reports. 2023;13:21941. doi: 10.1038/s41598-023-48109-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ottiger M, Soller M, Stocker RF, Kubli E. Binding sites of Drosophila melanogaster sex peptide pheromones. Journal of Neurobiology. 2000;44:57–71. [PubMed] [Google Scholar]
- Peng J, Chen S, Büsser S, Liu H, Honegger T, Kubli E. Gradual release of sperm bound sex-peptide controls female postmating behavior in Drosophila. Current Biology. 2005;15:207–213. doi: 10.1016/j.cub.2005.01.034. [DOI] [PubMed] [Google Scholar]
- Pfeiffer BD, Jenett A, Hammonds AS, Ngo TTB, Misra S, Murphy C, Scully A, Carlson JW, Wan KH, Laverty TR, Mungall C, Svirskas R, Kadonaga JT, Doe CQ, Eisen MB, Celniker SE, Rubin GM. Tools for neuroanatomy and neurogenetics in Drosophila. PNAS. 2008;105:9715–9720. doi: 10.1073/pnas.0803697105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phelps JS, Hildebrand DGC, Graham BJ, Kuan AT, Thomas LA, Nguyen TM, Buhmann J, Azevedo AW, Sustar A, Agrawal S, Liu M, Shanny BL, Funke J, Tuthill JC, Lee WCA. Reconstruction of motor control circuits in adult Drosophila using automated transmission electron microscopy. Cell. 2021;184:759–774. doi: 10.1016/j.cell.2020.12.013. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rezával C, Pavlou HJ, Dornan AJ, Chan YB, Kravitz EA, Goodwin SF. Neural circuitry underlying Drosophila female postmating behavioral responses. Current Biology. 2012;22:1155–1165. doi: 10.1016/j.cub.2012.04.062. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riabinina O, Vernon SW, Dickson BJ, Baines RA. Split-QF system for fine-tuned transgene expression in Drosophila. Genetics. 2019;212:53–63. doi: 10.1534/genetics.119.302034. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ribeiro C, Dickson BJ. Sex peptide receptor and neuronal TOR/S6K signaling modulate nutrient balancing in Drosophila. Current Biology. 2010;20:1000–1005. doi: 10.1016/j.cub.2010.03.061. [DOI] [PubMed] [Google Scholar]
- Rideout EJ, Dornan AJ, Neville MC, Eadie S, Goodwin SF. Control of sexual differentiation and behavior by the doublesex gene in Drosophila melanogaster. Nature Neuroscience. 2010;13:458–466. doi: 10.1038/nn.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rings A, Goodwin SF. To court or not to court - a multimodal sensory decision in Drosophila males. Current Opinion in Insect Science. 2019;35:48–53. doi: 10.1016/j.cois.2019.06.009. [DOI] [PubMed] [Google Scholar]
- Sakurai A, Koganezawa M, Yasunaga K, Emoto K, Yamamoto D. Select interneuron clusters determine female sexual receptivity in Drosophila. Nature Communications. 2013;4:1825. doi: 10.1038/ncomms2837. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheffer LK, Xu CS, Januszewski M, Lu Z, Takemura S-Y, Hayworth KJ, Huang GB, Shinomiya K, Maitlin-Shepard J, Berg S, Clements J, Hubbard PM, Katz WT, Umayam L, Zhao T, Ackerman D, Blakely T, Bogovic J, Dolafi T, Kainmueller D, Kawase T, Khairy KA, Leavitt L, Li PH, Lindsey L, Neubarth N, Olbris DJ, Otsuna H, Trautman ET, Ito M, Bates AS, Goldammer J, Wolff T, Svirskas R, Schlegel P, Neace E, Knecht CJ, Alvarado CX, Bailey DA, Ballinger S, Borycz JA, Canino BS, Cheatham N, Cook M, Dreher M, Duclos O, Eubanks B, Fairbanks K, Finley S, Forknall N, Francis A, Hopkins GP, Joyce EM, Kim S, Kirk NA, Kovalyak J, Lauchie SA, Lohff A, Maldonado C, Manley EA, McLin S, Mooney C, Ndama M, Ogundeyi O, Okeoma N, Ordish C, Padilla N, Patrick CM, Paterson T, Phillips EE, Phillips EM, Rampally N, Ribeiro C, Robertson MK, Rymer JT, Ryan SM, Sammons M, Scott AK, Scott AL, Shinomiya A, Smith C, Smith K, Smith NL, Sobeski MA, Suleiman A, Swift J, Takemura S, Talebi I, Tarnogorska D, Tenshaw E, Tokhi T, Walsh JJ, Yang T, Horne JA, Li F, Parekh R, Rivlin PK, Jayaraman V, Costa M, Jefferis GS, Ito K, Saalfeld S, George R, Meinertzhagen IA, Rubin GM, Hess HF, Jain V, Plaza SM. A connectome and analysis of the adult Drosophila central brain. eLife. 2020;9:e57443. doi: 10.7554/eLife.57443. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheunemann L, Lampin-Saint-Amaux A, Schor J, Preat T. A sperm peptide enhances long-term memory in female Drosophila. Science Advances. 2019;5:eaax3432. doi: 10.1126/sciadv.aax3432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt T, Choffat Y, Klauser S, Kubli E. The Drosophila melanogaster sex-peptide: a molecular analysis of structure-function relationships. Journal of Insect Physiology. 1993;39:361–368. doi: 10.1016/0022-1910(93)90023-K. [DOI] [Google Scholar]
- Schretter CE, Aso Y, Robie AA, Dreher M, Dolan MJ, Chen N, Ito M, Yang T, Parekh R, Branson KM, Rubin GM. Cell types and neuronal circuitry underlying female aggression in Drosophila. eLife. 2020;9:e58942. doi: 10.7554/eLife.58942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schütt C, Nöthiger R. Structure, function and evolution of sex-determining systems in Dipteran insects. Development. 2000;127:667–677. doi: 10.1242/dev.127.4.667. [DOI] [PubMed] [Google Scholar]
- Seidner G, Robinson JE, Wu M, Worden K, Masek P, Roberts SW, Keene AC, Joiner WJ. Identification of neurons with a privileged role in sleep homeostasis in Drosophila melanogaster. Current Biology. 2015;25:2928–2938. doi: 10.1016/j.cub.2015.10.006. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singh DND, Soller M. Venerose: a nuptial gift with implications. Journal of Neurogenetics. 2025;39:1–3. doi: 10.1080/01677063.2025.2473095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Soller M, Bownes M, Kubli E. Mating and sex peptide stimulate the accumulation of yolk in oocytes of Drosophila melanogaster. European Journal of Biochemistry. 1997;243:732–738. doi: 10.1111/j.1432-1033.1997.00732.x. [DOI] [PubMed] [Google Scholar]
- Soller M, Bownes M, Kubli E. Control of oocyte maturation in sexually mature Drosophila females. Developmental Biology. 1999;208:337–351. doi: 10.1006/dbio.1999.9210. [DOI] [PubMed] [Google Scholar]
- Soller M, Haussmann IU, Hollmann M, Choffat Y, White K, Kubli E, Schäfer MA. Sex-peptide-regulated female sexual behavior requires a subset of ascending ventral nerve cord neurons. Current Biology. 2006;16:1771–1782. doi: 10.1016/j.cub.2006.07.055. [DOI] [PubMed] [Google Scholar]
- Sorkaç A, Moșneanu RA, Crown AM, Savaş D, Okoro AM, Memiş E, Talay M, Barnea G. retro-Tango enables versatile retrograde circuit tracing in Drosophila. eLife. 2023;12:e85041. doi: 10.7554/eLife.85041. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stockinger P, Kvitsiani D, Rotkopf S, Tirián L, Dickson BJ. Neural circuitry that governs Drosophila male courtship behavior. Cell. 2005;121:795–807. doi: 10.1016/j.cell.2005.04.026. [DOI] [PubMed] [Google Scholar]
- Struhl G, Basler K. Organizing activity of wingless protein in Drosophila. Cell. 1993;72:527–540. doi: 10.1016/0092-8674(93)90072-x. [DOI] [PubMed] [Google Scholar]
- Sweeney ST, Broadie K, Keane J, Niemann H, O’Kane CJ. Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects. Neuron. 1995;14:341–351. doi: 10.1016/0896-6273(95)90290-2. [DOI] [PubMed] [Google Scholar]
- Talay M, Richman EB, Snell NJ, Hartmann GG, Fisher JD, Sorkaç A, Santoyo JF, Chou-Freed C, Nair N, Johnson M, Szymanski JR, Barnea G. Transsynaptic mapping of second-order taste neurons in flies by trans-Tango. Neuron. 2017;96:783–795. doi: 10.1016/j.neuron.2017.10.011. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wainwright SM, Hopkins BR, Mendes CC, Sekar A, Kroeger B, Hellberg JEEU, Fan S-J, Pavey A, Marie PP, Leiblich A, Sepil I, Charles PD, Thézénas ML, Fischer R, Kessler BM, Gandy C, Corrigan L, Patel R, Wigby S, Morris JF, Goberdhan DCI, Wilson C. Drosophila sex peptide controls the assembly of lipid microcarriers in seminal fluid. PNAS. 2021;118:e2019622118. doi: 10.1073/pnas.2019622118. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang F, Wang K, Forknall N, Parekh R, Dickson BJ. Circuit and behavioral mechanisms of sexual rejection by Drosophila females. Current Biology. 2020a;30:3749–3760. doi: 10.1016/j.cub.2020.07.083. [DOI] [PubMed] [Google Scholar]
- Wang F, Wang K, Forknall N, Patrick C, Yang T, Parekh R, Bock D, Dickson BJ. Neural circuitry linking mating and egg laying in Drosophila females. Nature. 2020b;579:101–105. doi: 10.1038/s41586-020-2055-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang K, Wang F, Forknall N, Yang T, Patrick C, Parekh R, Dickson BJ. Neural circuit mechanisms of sexual receptivity in Drosophila females. Nature. 2021;589:577–581. doi: 10.1038/s41586-020-2972-7. [DOI] [PubMed] [Google Scholar]
- White MA, Bonfini A, Wolfner MF, Buchon N. Drosophila melanogaster sex peptide regulates mated female midgut morphology and physiology. PNAS. 2021;118:e2018112118. doi: 10.1073/pnas.2018112118. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamada D, Ishimoto H, Li X, Kohashi T, Ishikawa Y, Kamikouchi A. GABAergic local interneurons shape female fruit fly response to mating songs. The Journal of Neuroscience. 2018;38:4329–4347. doi: 10.1523/JNEUROSCI.3644-17.2018. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto D, Koganezawa M. Genes and circuits of courtship behaviour in Drosophila males. Nature Reviews. Neuroscience. 2013;14:681–692. doi: 10.1038/nrn3567. [DOI] [PubMed] [Google Scholar]
- Yang CH, Rumpf S, Xiang Y, Gordon MD, Song W, Jan LY, Jan YN. Control of the postmating behavioral switch in Drosophila females by internal sensory neurons. Neuron. 2009;61:519–526. doi: 10.1016/j.neuron.2008.12.021. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yapici N, Kim YJ, Ribeiro C, Dickson BJ. A receptor that mediates the post-mating switch in Drosophila reproductive behaviour. Nature. 2008;451:33–37. doi: 10.1038/nature06483. [DOI] [PubMed] [Google Scholar]
- Zaharieva E, Haussmann IU, Bräuer U, Soller M. Concentration and localization of coexpressed ELAV/Hu proteins control specificity of mRNA processing. Molecular and Cellular Biology. 2015;35:3104–3115. doi: 10.1128/MCB.00473-15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhang SX, Glantz EH, Miner LE, Rogulja D, Crickmore MA. Hormonal control of motivational circuitry orchestrates the transition to sexuality in Drosophila. Science Advances. 2021;7:eabg6926. doi: 10.1126/sciadv.abg6926. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhou C, Pan Y, Robinett CC, Meissner GW, Baker BS. Central brain neurons expressing doublesex regulate female receptivity in Drosophila. Neuron. 2014;83:149–163. doi: 10.1016/j.neuron.2014.05.038. [DOI] [PubMed] [Google Scholar]