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. 2000 May;78(5):2257–2269. doi: 10.1016/S0006-3495(00)76772-5

Lateral diffusion of membrane proteins in the presence of static and dynamic corrals: suggestions for appropriate observables.

F L Brown 1, D M Leitner 1, J A McCammon 1, K R Wilson 1
PMCID: PMC1300817  PMID: 10777724

Abstract

We consider the possibility of inferring the nature of cytoskeletal interaction with transmembrane proteins via optical experiments such as single-particle tracking (SPT) and near-field scanning optical microscopy (NSOM). In particular, we demonstrate that it may be possible to differentiate between static and dynamic barriers to diffusion by examining the time-dependent variance and higher moments of protein population inside cytoskeletal "corrals." Simulations modeling Band 3 diffusion on the surface of erythrocytes provide a concrete demonstration that these statistical tools might prove useful in the study of biological systems.

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Selected References

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