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. 2002 Oct;83(4):1965–1973. doi: 10.1016/S0006-3495(02)73958-1

Real-time measurement of spontaneous antigen-antibody dissociation.

Simone Kulin 1, Rani Kishore 1, Joseph B Hubbard 1, Kristian Helmerson 1
PMCID: PMC1302286  PMID: 12324415

Abstract

We report observations in real time of thermally driven adhesion and dissociation between a monoclonal IgE antibody and its specific antigen N-epsilon-2,4-dinitrophenyl-L-lysine. Both molecules were attached to the surfaces of different polystyrene microspheres trapped by optical tweezers. Monitoring spontaneous successive attachment and detachment events allowed a direct determination of the reaction-limited detachment rate k(off) for a single bond and also for multiple bonds. We observed both positive and negative cooperativity between multiple bonds depending on whether the antigen was linked to the microsphere with or without a tether, respectively.

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Selected References

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