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. 1971 Jan;35(1):22–27.

A Comparison of Bluetongue Virus and EHD Virus: Electronmicroscopy and Serology

F C Thomas 1, Janice Miller 1
PMCID: PMC1319534  PMID: 4251410

Abstract

Bluetongue virus, BT8, and the virus of epizootic hemorrhagic disease (EHD) of deer, NJ-55, were plaque purified and compared electronmicroscopically and serologically. The latter included a plaque reduction neutralization test, the agar gel precipitin test, and the complement fixation test. The viruses were indistinguishable morphologically, but antigenically different. A plaquing technique was described for EHD virus.

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24Fig. 2D, E and F.
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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boulanger P., Ruckerbauer G. M., Bannister G. L., Gray D. P., Girard A. Studies on bluetongue. 3. Comparison of two complement-fixation methods. Can J Comp Med Vet Sci. 1967 Jul;31(7):166–170. [PMC free article] [PubMed] [Google Scholar]
  2. Els H. J., Verwoerd D. W. Morphology of bluetongue virus. Virology. 1969 Jun;38(2):213–219. doi: 10.1016/0042-6822(69)90362-6. [DOI] [PubMed] [Google Scholar]
  3. Howell P. G., Verwoerd D. W., Oellermann R. A. Plaque formation by bluetongue virus. Onderstepoort J Vet Res. 1967 Dec;34(2):317–332. [PubMed] [Google Scholar]
  4. Jochim M. M., Chow T. L. Immunodiffusion of bluetongue virus. Am J Vet Res. 1969 Jan;30(1):33–41. [PubMed] [Google Scholar]
  5. Karstad L., Trainer D. O. Histopathology of experimental bluetongue disease of white-tailed deer. Can Vet J. 1967 Nov;8(11):247–254. [PMC free article] [PubMed] [Google Scholar]
  6. Lecatsas G. Electron microscopic study of the formation of bluetongue virus. Onderstepoort J Vet Res. 1968 Jun;35(1):139–149. [PubMed] [Google Scholar]
  7. MOLLENHAUER H. H. PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY. Stain Technol. 1964 Mar;39:111–114. [PubMed] [Google Scholar]
  8. Pini A., Coackley W., Ohder H. The adverse effect of some calf sera on the isolation and propagation of bluetongue virus in tissue culture. Arch Gesamte Virusforsch. 1966;18(1):88–95. doi: 10.1007/BF01241704. [DOI] [PubMed] [Google Scholar]
  9. Russell P. K., Nisalak A., Sukhavachana P., Vivona S. A plaque reduction test for dengue virus neutralizing antibodies. J Immunol. 1967 Aug;99(2):285–290. [PubMed] [Google Scholar]
  10. Trainer D. O., Jochim M. M. Serologic evidence of bluetongue in wild ruminants of North America. Am J Vet Res. 1969 Nov;30(11):2007–2011. [PubMed] [Google Scholar]
  11. Tsai K. S., Karstad L. Epizootic hemorrhagic disease virus of deer: an electron microscopic study. Can J Microbiol. 1970 Jun;16(6):427–432. doi: 10.1139/m70-073. [DOI] [PubMed] [Google Scholar]
  12. Wilhelm A. R., Trainer D. O. Hematological and virological studies of epizootic hemorrhagic disease of deer. Wildl Dis. 1969 Apr;5(2):77–passim. doi: 10.7589/0090-3558-5.2.77. [DOI] [PubMed] [Google Scholar]
  13. Zeigel R. F., Rabotti G., Smith M. V. Electron microscopic observations on the association of viruses with membrane systems in hamster tumor cells propagated in tissue culture. J Natl Cancer Inst. 1969 Sep;43(3):653–669. [PubMed] [Google Scholar]

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