Skip to main content
PMC home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1974 Jul;38(3):303–313.

The Kinetics of Hematopoiesis in the Light Horse I. The Lifespan of Peripheral Blood Cells in the Normal Horse

E I Carter 1,2,3, V E Valli 1,2,3, B J McSherry 1,2,3, J H Lumsden 1,2,3, F J Milne 1,2,3, G A Robinson 1,2,3
PMCID: PMC1319873  PMID: 4277706

Abstract

Three Standardbred horses were given 0.2 mg (1 mCi) of 75selenomethionine intravenously and a second group of three were given 10 mCi of tritiated diisopropylfluorophosphate (0.5 mg) intravenously. Observations on labeled cells were continued for 250 days after radioselenium injection and 160 days after tritium injection.

The lifespan of erythrocytes using 75selenmethionine was 155 ± 10 days and 148 ± 7.8 days using tritiated diisopropylfluorophosphate. There was no significant difference at the 10% level between the lifespans, using these labels. The uptake of radioselenium into erythrocytes reached a mean maximum of 11.5% at 82 ± 18.9 days after injection of the label. There was an elution of from 17.6% of the injected dose of tritium label down to 7.5% eight days after injection of this isotope. From this study both of these labels appear to be satisfactory for determining the erythrocyte lifespan of the horse.

The mean time of the curve of mean radioselenium activity of peripheral blood leukocytes for the three horses was 5.54 days and the lifespan of these cells was seven days. The mean lifespan of peripheral blood leukocytes of the three horses after in vivo labelling with tritiated diisopropylfluorophosphate was 17.3 hours. No specific labelling was found in bone marrow or peripheral blood cells at this level of tritium labelling by dipping emulsion autoradiography.

The mean lifespan of equine platelets for three horses using radioselenomethionine was 9.2 days and the mean lifespan of equine platelets using tritiated diisopropylfluorophosphate was 6.6 days in a group of three horses.

Full text

PDF
303

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOVE J. R., EBAUGH F. G., Jr The use of diisopropylfluorophosphate 32 for the determination of in-vivo red cell survival and plasma cholinesterase turnover rates. J Lab Clin Med. 1958 Jun;51(6):916–925. [PubMed] [Google Scholar]
  2. Brodsky I., Siegel N. H., Kahn S. B., Ross E. M., Petkov G. Simultaneous fibrinogen and platelet survival with (75Se)selenomethionine in man. Studies in diseases with normal coagulation and in hepatocellular disease with abnormal coagulation. Br J Haematol. 1970 Mar;18(3):341–355. doi: 10.1111/j.1365-2141.1970.tb01448.x. [DOI] [PubMed] [Google Scholar]
  3. CARTWRIGHT G. E., ATHENS J. W., WINTROBE M. M. THE KINETICS OF GRANULOPOIESIS IN NORMAL MAN. Blood. 1964 Dec;24:780–803. [PubMed] [Google Scholar]
  4. CLINE M. J., BERLIN N. I. Measurement of red cell survival with tritiated diisopropylfluorophosphate. J Lab Clin Med. 1962 Nov;60:826–832. [PubMed] [Google Scholar]
  5. Carter E. I., Valli V. E., McSherry B. J. The separation of peripheral blood cells of the horse. Can J Comp Med. 1974 Jan;38(1):72–74. [PMC free article] [PubMed] [Google Scholar]
  6. Cheng H. F., Peterson R. E., Evans T. C. Incorporation of 75Se-selenomethionine in human peripheral lymphocytes as an in vitro test of globulin-synthesizing capacity. J Nucl Med. 1969 Feb;10(2):63–67. [PubMed] [Google Scholar]
  7. Ebbe S., Stohlman F., Jr, Donovan J., Howard D. Platelet survival in the rat as measured with tritium-labeled diisopropylfluorophosphate. J Lab Clin Med. 1966 Aug;68(2):233–243. [PubMed] [Google Scholar]
  8. Evatt B. L., Levin J. Measurement of thrombopoiesis in rabbits using 75selenomethionine. J Clin Invest. 1969 Sep;48(9):1615–1626. doi: 10.1172/JCI106127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HJORT P. F., PAPUTCHIS H., CHENEY B. Labeling of red blood cells with radioactive diisopropylfluorophosphate (DFP32): evidence for an initial release of label. J Lab Clin Med. 1960 Mar;55:416–424. [PubMed] [Google Scholar]
  10. MARCILESE N. A., VALSECCHI R. M., FIGUEIRAS H. D., CAMBEROS H. R., VARELA J. E. NORMAL BLOOD VOLUMES IN THE HORSE. Am J Physiol. 1964 Jul;207:223–227. doi: 10.1152/ajplegacy.1964.207.1.223. [DOI] [PubMed] [Google Scholar]
  11. Maloney M. A., Patt H. M. Granulocyte transit from bone marrow to blood. Blood. 1968 Feb;31(2):195–201. [PubMed] [Google Scholar]
  12. Marcilese N. A., Figueiras H. D., Kremenchuzky S., Valsecchi R. M., Camberos H. R., Varela J. E. Red cell survival time in the horse, determined with di-isopropyl-phosphorofluoridate-P. Am J Physiol. 1966 Aug;211(2):281–282. doi: 10.1152/ajplegacy.1966.211.2.281. [DOI] [PubMed] [Google Scholar]
  13. Marcilese N. A., Figueiras H. D., Valsecchi R. M., Fraga A. H., Camberos H. R., Varela J. E. Erythrokinetics in the horse. Am J Physiol. 1965 Oct;209(4):727–730. doi: 10.1152/ajplegacy.1965.209.4.727. [DOI] [PubMed] [Google Scholar]
  14. McGuire T. C., Henson J. B., Quist S. E. Viral-induced hemolysis in equine infectious anemia. Am J Vet Res. 1969 Dec;30(12):2091–2097. [PubMed] [Google Scholar]
  15. McIntyre P. A., Evatt B., Hodkinson B. A., Scheffel U. Selenium-75 seleno-methionine as a label for erythrocytes, leukocytes, and platelets in man. J Lab Clin Med. 1970 Mar;75(3):472–480. [PubMed] [Google Scholar]
  16. NEUBERGER A., NIVEN J. S. F. Haemoglobin formation in rabbits. J Physiol. 1951 Feb;112(3-4):292–310. doi: 10.1113/jphysiol.1951.sp004530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Najean Y., Ardaillou N. The use of 75Se-methionin for the in vivo study of platelet kinetics. Scand J Haematol. 1969;6(6):395–401. doi: 10.1111/j.1600-0609.1969.tb01833.x. [DOI] [PubMed] [Google Scholar]
  18. Penner J. A. Investigation of erythrocyte turnover with selenium-75-labeled methionine. J Lab Clin Med. 1966 Mar;67(3):427–438. [PubMed] [Google Scholar]
  19. Perry S., Godwin H. A., Zimmerman T. S. Physiology of the granulocyte. 2. JAMA. 1968 Mar 18;203(12):1025–1032. doi: 10.1001/jama.203.12.1025. [DOI] [PubMed] [Google Scholar]
  20. Sears D. A., Weed R. I. Diisopropylfluorophosphate is not a specific label for the red cell membrane. Blood. 1969 Sep;34(3):376–379. [PubMed] [Google Scholar]
  21. VAN PUTTEN L. M. The life span of red cells in the rat and the mouse as determined by labeling with DFP32 in vivo. Blood. 1958 Aug;13(8):789–794. [PubMed] [Google Scholar]
  22. Valli V. E., Hulland T. J., McSherry B. J., Robinson G. A., Gilman J. P. The kinetics of haematopoiesis in the calf. I. An autoradiographical study of myelopoiesis in normal, anaemic and endotoxin treated calves. Res Vet Sci. 1971 Nov;12(6):535–550. [PubMed] [Google Scholar]
  23. Wu A. M., Till J. E., Siminovitch L., McCulloch E. A. Cytological evidence for a relationship between normal hemotopoietic colony-forming cells and cells of the lymphoid system. J Exp Med. 1968 Mar 1;127(3):455–464. doi: 10.1084/jem.127.3.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yoshida Y., Todo A., Shirakawa S., Wakisaka G., Uchino H. Proliferation of megaloblasts in pernicious anemia as observed from nucleic acid metabolism. Blood. 1968 Mar;31(3):292–303. [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES