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Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1980 Oct;44(4):440–446.

Serotypic and biochemical characterization of Bacteroides nodosus isolates from Oregon.

J A Schmitz, J L Gradin
PMCID: PMC1320102  PMID: 7448630

Abstract

Ninety-seven Bacteroides nodosus isolates were characterized by the tube agglutination test. Fourteen serotypes were identified including isolates that were serologically similar to Australian serotypes A, B and C. One additional isolate remains untyped and possibly represents another serotype. The isolates were cultured from 20 different flocks. Multiple isolates were obtained from 15 of the flocks and 13 of these had two to seven different B. nodosus serotypes. Eleven B. nodosus isolates representing one Australian and ten Oregon serotypes were nonfermentative in various carbohydrates and did not produce indole. These isolates all exhibited proteolytic activity. The prototype strains of 12 of the 14 serotypes demonstrated virulence as assessed by an elastase production assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Egerton J. R., Burrell D. H. Prophylactic and therapeutic vaccination against ovine foot-rot. Aust Vet J. 1970 Nov;46(11):517–522. doi: 10.1111/j.1751-0813.1970.tb06636.x. [DOI] [PubMed] [Google Scholar]
  2. Egerton J. R., Morgan I. R. Treatment and prevention of foot-rot in sheep with Fusiformis nodosus vaccine. Vet Rec. 1972 Nov 4;91(19):453–457. doi: 10.1136/vr.91.19.453. [DOI] [PubMed] [Google Scholar]
  3. Egerton J. R., Roberts D. S., Parsonson I. M. The aetiology and pathogenesis of ovine foot-rot. I. A histological study of the bacterial invasion. J Comp Pathol. 1969 Apr;79(2):207–215. doi: 10.1016/0021-9975(69)90007-3. [DOI] [PubMed] [Google Scholar]
  4. Egerton J. R. Significance of Fusiformis nodosus serotypes in resistance of vaccinated sheep to experimental foot-rot. Aust Vet J. 1974 Feb;50(2):59–62. doi: 10.1111/j.1751-0813.1974.tb05252.x. [DOI] [PubMed] [Google Scholar]
  5. Egerton J. R. Surface and somatic antigens of Fusiformis nodosus. J Comp Pathol. 1973 Jan;83(1):151–159. doi: 10.1016/0021-9975(73)90038-8. [DOI] [PubMed] [Google Scholar]
  6. Egerton J. R., Thompson J. J., Merritt G. C. Comparison of oil adjuvant and alum precipitated Bacteroides nodosus vaccines in treatment of foot-rot. Vet Rec. 1979 Feb 3;104(5):98–100. doi: 10.1136/vr.104.5.98. [DOI] [PubMed] [Google Scholar]
  7. Gradin J. L., Schmitz J. A. Selective medium for isolation of Bacteroides nodosus. J Clin Microbiol. 1977 Sep;6(3):298–302. doi: 10.1128/jcm.6.3.298-302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kerry J. B., Craig G. R. Effect of vaccination against foot rot in young sheep wintered in straw yards. Vet Rec. 1976 May 29;98(22):446–447. doi: 10.1136/vr.98.22.446. [DOI] [PubMed] [Google Scholar]
  9. Roberts D. S., Egerton J. R. The aetiology and pathogenesis of ovine foot-rot. II. The pathogenic association of Fusiformis nodosus and F. necrophorus. J Comp Pathol. 1969 Apr;79(2):217–227. doi: 10.1016/0021-9975(69)90008-5. [DOI] [PubMed] [Google Scholar]
  10. Roberts D. S., Foster W. H., Kerry J. B., Calder H. A. An alum-treated vaccine for the control of foot-rod in sheep. Vet Rec. 1972 Oct 28;91(18):428–429. doi: 10.1136/vr.91.18.428. [DOI] [PubMed] [Google Scholar]
  11. Schreckenberger P. C., Blazevic D. J. Rapid fermentation testing of anaerobic bacteria. J Clin Microbiol. 1976 Mar;3(3):313–317. doi: 10.1128/jcm.3.3.313-317.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schreckenberger P. C., Blazevic D. J. Rapid methods for biochemical testing of anaerobic bacteria. Appl Microbiol. 1974 Nov;28(5):759–762. doi: 10.1128/am.28.5.759-762.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Skerman T. M., Cairney I. M. Experimental observations on prophylactic and therapeutic vaccination against foot-rot in sheep. N Z Vet J. 1972 Nov;20(11):205–211. doi: 10.1080/00480169.1972.34052. [DOI] [PubMed] [Google Scholar]
  14. Stewart D. J. Studies on the antigenic structure of Bacteroides nodosus. Res Vet Sci. 1978 May;24(3):293–299. [PubMed] [Google Scholar]
  15. Stewart D. J. The role of elastase in the differentiation of Bacteroides nodosus infections in sheep and cattle. Res Vet Sci. 1979 Jul;27(1):99–105. [PubMed] [Google Scholar]
  16. Stewart D. J. The role of various antigenic fractions of Bacteroides nodosus in eliciting protection against foot-rot in vaccinated sheep. Res Vet Sci. 1978 Jan;24(1):14–19. [PubMed] [Google Scholar]
  17. Thorley C. M. A simplified method for the isolation of Bacteroides nodusus from ovine foot-rot and studies on its colony morphology and serology. J Appl Bacteriol. 1976 Jun;40(3):301–309. doi: 10.1111/j.1365-2672.1976.tb04178.x. [DOI] [PubMed] [Google Scholar]

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