Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1981 Jul;35(1):1–16. doi: 10.1016/S0006-3495(81)84769-8

The thermotropic behavior of dipalmitoyl phosphatidylcholine bilayers. A Fourier transform infrared study of specifically labeled lipids.

D G Cameron, H L Casal, H H Mantsch, Y Boulanger, I C Smith
PMCID: PMC1327498  PMID: 6894878

Abstract

Fourier transform infrared spectroscopy has been used to study the thermotropic behavior of hydrated multibilayers of specifically deuterated derivatives of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. It is shown that throughout the gel phase there is little or no conformational disorder introduced into the acyl chains. The pretransition effect is greatest in the central segments of the acyl chains, demonstrating that the interchain interactions are more pronounced in this region than in the center of the bilayer and suggesting the presence, in the gel phase, of a "plateau" in the strength of the interchain interactions. As the temperature is reduced, the rate of rotation of the terminal methyl group decreases steadily; below 0 degree C the conformation is rigid on the infrared time scale.

Full text

PDF
5

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asher I. M., Levin I. W. Effects of temperature and molecular interactions on the vibrational infrared spectra of phospholipid vesicles. Biochim Biophys Acta. 1977 Jul 4;468(1):63–72. doi: 10.1016/0005-2736(77)90151-1. [DOI] [PubMed] [Google Scholar]
  2. Bansil R., Day J., Meadows M., Rice D., Oldfield E. Laser Raman spectroscopic study of specifically deuterated phospholipid bilayers. Biochemistry. 1980 Apr 29;19(9):1938–1943. doi: 10.1021/bi00550a033. [DOI] [PubMed] [Google Scholar]
  3. Cameron D. G., Casal H. L., Gudgin E. F., Mantsch H. H. The gel phase of dipalmitoyl phosphatidylcholine. An infrared characterization of the acyl chain packing. Biochim Biophys Acta. 1980 Mar 13;596(3):463–467. doi: 10.1016/0005-2736(80)90135-2. [DOI] [PubMed] [Google Scholar]
  4. Cameron D. G., Casal H. L., Mantsch H. H. Characterization of the pretransition in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine by Fourier transform infrared spectroscopy. Biochemistry. 1980 Aug 5;19(16):3665–3672. doi: 10.1021/bi00557a005. [DOI] [PubMed] [Google Scholar]
  5. Cameron D. G., Casal H. L., Mantsch H. H. The application of fourier transform infrared transmission spectroscopy to the study of model and natural membranes. J Biochem Biophys Methods. 1979;1(1):21–36. doi: 10.1016/0165-022x(79)90043-5. [DOI] [PubMed] [Google Scholar]
  6. Davis J. H. Deuterium magnetic resonance study of the gel and liquid crystalline phases of dipalmitoyl phosphatidylcholine. Biophys J. 1979 Sep;27(3):339–358. doi: 10.1016/S0006-3495(79)85222-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gaber B. P., Peticolas W. L. On the quantitative interpretation of biomembrane structure by Raman spectroscopy. Biochim Biophys Acta. 1977 Mar 1;465(2):260–274. doi: 10.1016/0005-2736(77)90078-5. [DOI] [PubMed] [Google Scholar]
  8. Gaber B. P., Yager P., Peticolas W. L. Conformational nonequivalence of chains 1 and 2 of dipalmitoyl phosphatidylcholine as observed by Raman spectroscopy. Biophys J. 1978 Dec;24(3):677–688. doi: 10.1016/S0006-3495(78)85413-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gruen D. W. A statistical mechanical model of the lipid bilayer above its phase transition. Biochim Biophys Acta. 1980 Jan 25;595(2):161–183. doi: 10.1016/0005-2736(80)90081-4. [DOI] [PubMed] [Google Scholar]
  10. Janiak M. J., Small D. M., Shipley G. G. Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin. Biochemistry. 1976 Oct 19;15(21):4575–4580. doi: 10.1021/bi00666a005. [DOI] [PubMed] [Google Scholar]
  11. Marsh D. Molecular motion in phospholipid bilayers in the gel phase: long axis rotation. Biochemistry. 1980 Apr 15;19(8):1632–1637. doi: 10.1021/bi00549a017. [DOI] [PubMed] [Google Scholar]
  12. Pearson R. H., Pascher I. The molecular structure of lecithin dihydrate. Nature. 1979 Oct 11;281(5731):499–501. doi: 10.1038/281499a0. [DOI] [PubMed] [Google Scholar]
  13. Pink D. A., Green T. J., Chapman D. Raman scattering in bilayers of saturated phosphatidylcholines. Experiment and theory. Biochemistry. 1980 Jan 22;19(2):349–356. doi: 10.1021/bi00543a016. [DOI] [PubMed] [Google Scholar]
  14. Seelig A., Seelig J. Bilayers of dipalmitoyl-3-sn-phosphatidylcholine. Conformational differences between the fatty acyl chains. Biochim Biophys Acta. 1975 Sep 16;406(1):1–5. doi: 10.1016/0005-2736(75)90037-1. [DOI] [PubMed] [Google Scholar]
  15. Seelig A., Seelig J. The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance. Biochemistry. 1974 Nov 5;13(23):4839–4845. doi: 10.1021/bi00720a024. [DOI] [PubMed] [Google Scholar]
  16. Seelig J. Deuterium magnetic resonance: theory and application to lipid membranes. Q Rev Biophys. 1977 Aug;10(3):353–418. doi: 10.1017/s0033583500002948. [DOI] [PubMed] [Google Scholar]
  17. Stockton G. W., Polnaszek C. F., Tulloch A. P., Hasan F., Smith I. C. Molecular motion and order in single-bilayer vesicles and multilamellar dispersions of egg lecithin and lecithin-cholesterol mixtures. A deuterium nuclear magnetic resonance study of specifically labeled lipids. Biochemistry. 1976 Mar 9;15(5):954–966. doi: 10.1021/bi00650a003. [DOI] [PubMed] [Google Scholar]
  18. Yellin N., Levin I. W. Hydrocarbon trans-gauche isomerization in phospholipid bilayer gel assemblies. Biochemistry. 1977 Feb 22;16(4):642–647. doi: 10.1021/bi00623a014. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES