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. 1980 May;30(2):317–325. doi: 10.1016/S0006-3495(80)85097-1

Comparison of polyelectrolyte theories of the binding of cations to DNA.

R W Wilson, D C Rau, V A Bloomfield
PMCID: PMC1328737  PMID: 7260278

Abstract

Predictions of the binding of counterions to DNA made using the counterion condensation theory developed by Manning are compared with those made using the Poisson-Boltzmann equation, solved numerically by the Runge-Kutta procedure. Ions are defined as territorially or atmospherically bound if they fall within a given distance, defined by counterion condensation theory, from the DNA surface. Two types of experimental situations are considered. The first is the delocalized binding of a single type of counterion to DNA. In this case the Poisson-Boltzmann treatment predicts somewhat lower extents of binding TO DNA, modeled as a 10-A radius cylinder, than does Manning theory. The two theories converge as the radius decreases. The second type of experiment is the competition of ions of different valence for binding to DNA. The theories are compared with literature values of binding constants of divalent ions in the presence of monovalent ions, and of spermidine 3+ in the presence of Na+ or Mg2+. Both predict with fair accuracy the salt dependence of the equilibrium constants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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