Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1982 Jun;38(3):217–226. doi: 10.1016/S0006-3495(82)84551-7

Amplitude of Rippling in the Pβ Phase of Dipalmitoylphosphatidylcholine Bilayers

J Stamatoff, B Feuer, H J Guggenheim, G Tellez, T Yamane
PMCID: PMC1328861  PMID: 6896664

Abstract

We present x-ray diffraction results of dipalmitoylphosphatidylcholine (DPPC) multilayers in three structural phases. Using pure DPPC, precision temperature control, and high angular resolution methods, we have discovered splitting of the first diffraction order due to multilayering in the Pβ phase. This splitting permits us to calculate the amplitude of ripples in this phase. The amplitude is large enough to suggest a structural mechanism for rippling.

Full text

PDF
223

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albon N., Sturtevant J. M. Nature of the gel to liquid crystal transition of synthetic phosphatidylcholines. Proc Natl Acad Sci U S A. 1978 May;75(5):2258–2260. doi: 10.1073/pnas.75.5.2258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Büldt G., Gally H. U., Seelig A., Seelig J., Zaccai G. Neutron diffraction studies on selectively deuterated phospholipid bilayers. Nature. 1978 Jan 12;271(5641):182–184. doi: 10.1038/271182a0. [DOI] [PubMed] [Google Scholar]
  3. Cubero Robles E., van den Berg D. Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides. Biochim Biophys Acta. 1969 Dec 17;187(4):520–526. doi: 10.1016/0005-2760(69)90049-6. [DOI] [PubMed] [Google Scholar]
  4. Graddick W. F., Stamatoff J. B., Eisenberger P., Berreman D. W., Spielberg N. Order-disorder and the pretransition in dipalmitoyl phosphatidylcholine multilayers. Biochem Biophys Res Commun. 1979 Jun 13;88(3):907–912. doi: 10.1016/0006-291x(79)91494-3. [DOI] [PubMed] [Google Scholar]
  5. Janiak M. J., Small D. M., Shipley G. G. Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin. Biochemistry. 1976 Oct 19;15(21):4575–4580. doi: 10.1021/bi00666a005. [DOI] [PubMed] [Google Scholar]
  6. Janiak M. J., Small D. M., Shipley G. G. Temperature and compositional dependence of the structure of hydrated dimyristoyl lecithin. J Biol Chem. 1979 Jul 10;254(13):6068–6078. [PubMed] [Google Scholar]
  7. Luna E. J., McConnell H. M. The intermediate monoclinic phase of phosphatidylcholines. Biochim Biophys Acta. 1977 May 2;466(3):381–392. doi: 10.1016/0005-2736(77)90331-5. [DOI] [PubMed] [Google Scholar]
  8. McIntosh T. J., Costello M. J. Effects of n-alkanes on the morphology of lipid bilayers. A freeze-fracture and negative stain analysis. Biochim Biophys Acta. 1981 Jul 20;645(2):318–326. doi: 10.1016/0005-2736(81)90203-0. [DOI] [PubMed] [Google Scholar]
  9. McIntosh T. J. Differences in hydrocarbon chain tilt between hydrated phosphatidylethanolamine and phosphatidylcholine bilayers. A molecular packing model. Biophys J. 1980 Feb;29(2):237–245. doi: 10.1016/S0006-3495(80)85128-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Parsegian V. A., Rand R. P., Stamatoff J. Perturbation of membrane structure by uranyl acetate labeling. Biophys J. 1981 Mar;33(3):475–477. doi: 10.1016/S0006-3495(81)84908-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Powers L., Pershan P. S. Monodomain samples of dipalmitoyl phosphatidylcholine with varying concentrations of water and other ingredients. Biophys J. 1977 Nov;20(2):137–152. doi: 10.1016/S0006-3495(77)85540-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rand R. P., Chapman D., Larsson K. Tilted hydrocarbon chains of dipalmitoyl lecithin become perpendicular to the bilayer before melting. Biophys J. 1975 Nov;15(11):1117–1124. doi: 10.1016/S0006-3495(75)85888-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SINGLETON W. S., GRAY M. S., BROWN M. L., WHITE J. L. CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS. J Am Oil Chem Soc. 1965 Jan;42:53–56. doi: 10.1007/BF02558256. [DOI] [PubMed] [Google Scholar]
  14. Selinger Z., Lapidot Y. Synthesis of fatty acid anhydrides by reaction with dicyclohexylcarbodiimide. J Lipid Res. 1966 Jan;7(1):174–175. [PubMed] [Google Scholar]
  15. Stamatoff J., Bilash T., Ching Y., Eisenberger P. X-ray scattering from labeled membranes. Biophys J. 1979 Dec;28(3):413–421. doi: 10.1016/S0006-3495(79)85190-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tardieu A., Luzzati V., Reman F. C. Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases. J Mol Biol. 1973 Apr 25;75(4):711–733. doi: 10.1016/0022-2836(73)90303-3. [DOI] [PubMed] [Google Scholar]
  17. Torbet J., Wilkins M. H. X-ray diffraction studies of lecithin bilayers. J Theor Biol. 1976 Oct 21;62(2):447–458. doi: 10.1016/0022-5193(76)90129-6. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES