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. 1985 Oct;48(4):623–631. doi: 10.1016/S0006-3495(85)83818-2

Time-resolved fluorescence polarization from ordered biological assemblies.

T P Burghardt
PMCID: PMC1329339  PMID: 4052569

Abstract

We calculate the time dependence of the polarized fluorescence signal from fluorescent-labeled elements of a biological assembly that are rotationally diffusing in an arbitrary three-dimensional angular potential. We have formulated this calculation using the model-independent description of the angular potential wherein the angular potential is described by an expansion in a complete set of orthonormal functions with the expansion coefficients (or order parameters) determined by time-independent methods (Burghardt, T. P., 1984, Biopolymers, 23:2382-2406). We have applied the calculation to fluorescent-labeled myosin cross-bridges in relaxed muscle fibers. In a related paper we describe the experimental observation of the myosin cross-bridges rotationally diffusing in an angular potential (Burghardt, T. P., and N. L. Thompson, 1985, Biochemistry, 24:3731-3735).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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