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. 1985 Sep;48(3):533–537. doi: 10.1016/S0006-3495(85)83810-8

Estimation of intracellular [Ca2+] by nonlinear indicators. A quantitative analysis.

D T Yue, W G Wier
PMCID: PMC1329368  PMID: 4041543

Abstract

When spatial gradients of intracellular free [Ca2+] are present, intracellular calcium indicators that have a nonlinear response to [Ca2+] may yield an estimate of [Ca2+] that differs from the spatial average [Ca2+]. We present two rules that provide (a) general criteria to distinguish those classes of indicators that will yield an overestimate of spatial average [Ca2+] from those that will yield an underestimate, and (b) limits on the extent to which spatial average [Ca2+] might be over- or underestimated. These rules are used to interpret quantitatively the aequorin luminescence signals obtained from cardiac ventricular myocardium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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