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. 1987 Oct;52(4):571–576. doi: 10.1016/S0006-3495(87)83246-0

Activity-dependent calcium transients in central nervous system myelinated axons revealed by the calcium indicator Fura-2.

V Lev-Ram 1, A Grinvald 1
PMCID: PMC1330047  PMID: 3676438

Abstract

Optical measurements from rat optic nerve, loaded with the new Ca2+ indicator Fura-2, provide the first evidence for the presence of activity-dependent fast intracellular [Ca2+] transients in mammalian central nervous system (CNS) myelinated axons. The results suggest that voltage-dependent Ca2+ channels are present in some of the myelinated axons. Optical measurements from axons stained with anterogradely transported voltage-sensitive dye suggest the presence of Ca2+-dependent potassium conductances in these axons. This report also demonstrates that Fura-2 can readily detect changes in [Ca2+] inside cells as a result of electrical activity, and establishes its suitability for measurements of intracellular Ca2+ transients in the millisecond time domain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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