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. 1974 Dec;243(3):831–846.1. doi: 10.1113/jphysiol.1974.sp010779

Pancreatic acinar cells: effects of lanthanum ions on amylase release and calcium ion fluxes

D E Chandler, J A Williams
PMCID: PMC1330737  PMID: 4449083

Abstract

1. The effect of La3+ on amylase release and Ca2+ fluxes in mouse pancreatic fragments in vitro was studied.

2. Amylase release was increased by 0·1 mM-La3+ and progressively inhibited by 1·0-10 mM-La3+. Non-stimulated and bethanecol stimulated secretion were altered in an identical manner. Inhibition of amylase release was rapid and reversible.

3. Uptake of 45Ca2+ was multiphasic with equilibrium with stable Ca2+ still not complete after 2 hr. La3+ (10 mM) limited uptake of 45Ca2+ to the extracellular space and slightly decreased total Ca2+ content. Lower concentrations of La3+ affected 45Ca2+ uptake and total Ca2+ content in a biphasic manner which paralleled effects on amylase release.

4. La3+ restricted washout of 45Ca2+ to isotope in the extracellular space and abolished the bethanecol-stimulated increase in 45Ca2+ efflux.

5. Uptake of 45Ca2+ into intracellular space, as measured by the `lanthanum' method, was not affected by bethanecol.

6. Tissue ultrastructure and Na+ and K+ content were not affected by La3+.

7. It is concluded that an influx of extracellular Ca2+ is not important for triggering of secretion and that La3+ may inhibit amylase release by acting on the release process rather than on Ca2+ influx.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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