Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1974 Sep;241(2):437–451. doi: 10.1113/jphysiol.1974.sp010665

Histamine metabolism of the gastric mucosa following antrectomy

L Lundell
PMCID: PMC1331041  PMID: 4443925

Abstract

1. Histamine metabolism of the gastric mucosa in rats subjected to antrectomy, antrectomy and substitution with pentagastrin and exclusion of the antrum has been investigated employing various in vivo and in vitro methods.

2. Mucosal histamine formation after antrectomy fell to about one third, whereas after antrum exclusion histamine formation increased many-fold.

3. After antrectomy, mucosal histamine content decreased, and increased after antrum exclusion.

4. After total gastrectomy, (a) whole-body histamine formation was reduced to about half, as judged by determining histamine excretion, and (b) pentagastrin infusion did not increase histamine excretion, showing absence of histamine mobilization from extra-gastric sources. In rats with the stomach retained, infusion of pentagastrin induced a dose-dependent increase in histamine excretion.

5. Kinetic studies in which [14C]histidine was injected and the resulting urinary [14C]histamine determined showed that on pentagastrin infusion after antrectomy newly formed histamine was initially mobilized to a larger extent than in the controls.

6. Antrectomized rats were subjected to substitution treatment by three injections per day of pentagastrin. After 3 weeks of substitution, histamine excretion was considerably higher than without substitution. After 6 weeks of substitution, histamine excretion was about the same in the substituted antrectomized, non-substituted antrectomized and sham-operated groups. Neither time nor substitution could, however, normalize the excretion of histamine on pentagastrin stimulation after antrectomy.

7. In non-substituted antrectomized rats, pentagastrin was less effective in elevating mucosal histamine formation than in the substituted and sham-operated groups.

8. The indispensability of the rat in this kind of study is emphasized.

Full text

PDF
441

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Accary J. P., Dubrasquet M., Bonfils S. Dosage radio-immunologique de la gastrine chez l'homme et chez l'animal. Nouv Presse Med. 1973 Jan 6;2(1):48–49. [PubMed] [Google Scholar]
  2. Aures D., Johnson L. R., Way L. W. Gastrin: obligatory intermediate for activation of gastric histidine decarboxylase in the rat. Am J Physiol. 1970 Jul;219(1):214–216. doi: 10.1152/ajplegacy.1970.219.1.214. [DOI] [PubMed] [Google Scholar]
  3. Capoferro R., Nygaard K. Effects of antrectomy on the gastric mucosa of the rat. Scand J Gastroenterol. 1973;8(4):347–352. [PubMed] [Google Scholar]
  4. Crean G. P., Marshall M. W., Rumsey R. D. Parietal cell hyperplasia induced by the administration of pentagastrin (ICI 50,123) to rats. Gastroenterology. 1969 Aug;57(2):147–155. [PubMed] [Google Scholar]
  5. FELDBERG W., TALESNIK J. Reduction of tissue histamine by compound 48/80. J Physiol. 1953 Jun 29;120(4):550–568. doi: 10.1113/jphysiol.1953.sp004917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GUSTAFSSON B., KAHLSON G., ROSENGREN E. Biogenesis of histamine studied by its distribution and urinary excretion in germ free reared and not germ free rats fed a histamine free diet. Acta Physiol Scand. 1957 Dec 7;41(2-3):217–228. doi: 10.1111/j.1748-1716.1957.tb01522.x. [DOI] [PubMed] [Google Scholar]
  7. HIGH D. P., SHEPHERD D. M., WOODCOCK B. G. URINARY HISTAMINE EXCRETION IN THE GASTRECTOMISED RAT. Life Sci. 1965 Apr;4:787–795. doi: 10.1016/0024-3205(65)90311-5. [DOI] [PubMed] [Google Scholar]
  8. Håkanson R., Liedberg G. Effect of pentagastrin and feeding on gastric histidine decarboxylase activity in the antrectomized rat. Eur J Pharmacol. 1972 Apr;18(1):31–36. doi: 10.1016/0014-2999(72)90128-8. [DOI] [PubMed] [Google Scholar]
  9. Håkanson R., Liedberg G. The role of endogenous gastrin in the activation of gastric histidine decarboxylase in the rat. Effect of antrectomy and vagal denervation. Eur J Pharmacol. 1970 Sep 1;12(1):94–103. doi: 10.1016/0014-2999(70)90033-6. [DOI] [PubMed] [Google Scholar]
  10. Johnson L. R., Aures D., Hakanson R. Effect of gastrin on the in vivo incorporation of 14C-leucine into protein of the digestive tract. Proc Soc Exp Biol Med. 1969 Dec;132(3):996–998. doi: 10.3181/00379727-132-34353. [DOI] [PubMed] [Google Scholar]
  11. Johnson L. R., Aures D., Yuen L. Pentagastrin-induced stimulation of protein synthesis in the gastrointestinal tract. Am J Physiol. 1969 Jul;217(1):251–254. doi: 10.1152/ajplegacy.1969.217.1.251. [DOI] [PubMed] [Google Scholar]
  12. Johnson L. R., Chandler A. M. RNA and DNA of gastric and duodenal mucosa in antrectomized and gastrin-treated rats. Am J Physiol. 1973 Apr;224(4):937–940. doi: 10.1152/ajplegacy.1973.224.4.937. [DOI] [PubMed] [Google Scholar]
  13. Johnson L. R., Jones R. S., Aures D., Håkanson R. Effect of antrectomy on gastric histidine decarboxylase activity in the rat. Am J Physiol. 1969 May;216(5):1051–1053. doi: 10.1152/ajplegacy.1969.216.5.1051. [DOI] [PubMed] [Google Scholar]
  14. KAHLSON G., ROSENGREN E., SVAHN D., THUNBERG R. MOBILIZATION AND FORMATION OF HISTAMINE IN THE GASTRIC MUCOSA AS RELATED TO ACID SECRETION. J Physiol. 1964 Nov;174:400–416. doi: 10.1113/jphysiol.1964.sp007494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. KAHLSON G., ROSENGREN E., THUNBERG R. OBSERVATIONS ON THE INHIBITION OF HISTAMINE FORMATION. J Physiol. 1963 Dec;169:467–486. doi: 10.1113/jphysiol.1963.sp007273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KOBAYASHI Y. Determination of histidine decarboxylase activity by liquid scintillation counting of C-14-O2. Anal Biochem. 1963 Apr;5:284–290. doi: 10.1016/0003-2697(63)90080-0. [DOI] [PubMed] [Google Scholar]
  17. Kragelund E., Amdrup E., Andreassen J., Jensen H. E. Pentapeptide and insulin stimulated gastric acid secretion in patients with gastric ulcer before and after precise antrectomy. Ann Surg. 1972 Nov;176(5):659–662. doi: 10.1097/00000658-197211000-00016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lundell L. Proceedings: Changes in urinary histamine following antrectomy and substitution with pentagastrin. J Physiol. 1974 Jan;236(1):42P–43P. [PubMed] [Google Scholar]
  19. Martin F., Macleod I. B., Sircus W. Effects of antrectomy on the fundic mucosa of the rat. Gastroenterology. 1970 Sep;59(3):437–444. [PubMed] [Google Scholar]
  20. OLBE L. EFFECT OF RESECTION OF GASTRIN RELEASING REGIONS ON ACID RESPONSE TO SHAM FEEDING AND INSULIN HYPOGLYCEMIA IN PAVLOV POUCH DOGS. Acta Physiol Scand. 1964 Sep-Oct;62:169–175. doi: 10.1111/j.1748-1716.1964.tb03965.x. [DOI] [PubMed] [Google Scholar]
  21. Rosengren E., Svensson S. E. The role of the antrum and the vagus nerve in the formation of gastric mucosal histamine. J Physiol. 1969 Nov;205(2):275–288. doi: 10.1113/jphysiol.1969.sp008964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SCHAYER R. W., DAVIS K. J., SMILEY R. L. Binding of histamine in vitro and its inhibition by cortisone. Am J Physiol. 1955 Jul;182(1):54–56. doi: 10.1152/ajplegacy.1955.182.1.54. [DOI] [PubMed] [Google Scholar]
  23. Shepherd D. M., Woodcock B. G. Histamine formation in normal and totally-gastrectomised rats. Arch Int Pharmacodyn Ther. 1968 Jul;174(1):50–61. [PubMed] [Google Scholar]
  24. Snyder S. H., Epps L. Regulation of histidine decarboxylase in rat stomach by gastrin: the effect of inhibitors of protein synthesis. Mol Pharmacol. 1968 Mar;4(2):187–195. [PubMed] [Google Scholar]
  25. Svensson S. E. Secretory behaviour and histamine formation in the rat Heidenhain pouch following antrectomy. J Physiol. 1970 May;207(3):699–708. doi: 10.1113/jphysiol.1970.sp009089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Svensson S. E. The secretory pattern of three stomach preparations in the rat. J Physiol. 1970 Apr;207(2):329–350. doi: 10.1113/jphysiol.1970.sp009064. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES