Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1973 Jan;228(2):361–376. doi: 10.1113/jphysiol.1973.sp010091

The effect of chronic duct ligation on the vascular and secretory responses of the cat's submaxillary gland

A C Darke, L H Smaje
PMCID: PMC1331302  PMID: 4687102

Abstract

1. The object of the present experiments was to re-investigate the role of kallikrein in functional hyperaemia in the submaxillary gland of the cat. Kallikrein concentration in the gland and saliva was reduced by ligation of the duct for 2-3 days before the acute experiment, at which time the duct was cannulated proximal to the ligation and blood flow and salivation measured.

2. Despite the fall in kallikrein concentration, a normal two-phase vascular response was obtained on continuous stimulation of the chorda-lingual nerve at 1-5 Hz and the progressive increase in blood flow to a constant level characteristic of 10 and 20 Hz stimulation was also seen. Quantitatively, the increase in blood flow following a 10 sec stimulation was reduced to about 75% of control values at all stimulation frequencies used and the maintained vasodilatation was reduced when using continuous stimulation at 1 Hz but not at 10 and 20 Hz.

3. The supersensitivity to the vasodilator properties of bradykinin, previously reported to follow duct ligation, was confirmed.

4. The increased blood flow produced by chorda-lingual nerve stimulation could be delayed by circulatory arrest. Evidence was obtained suggesting that the vascular response to chorda stimulation delayed by arterial occlusion was not mediated by kallikrein alone.

5. It was concluded that kallikrein was not the sole mediator of functional hyperaemia in the cat submaxillary gland but that a combination of vasodilator nerves and kallikrein release explained more phenomena than either hypothesis alone.

Full text

PDF
363

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMUNDSEN E., NUSTAD K., WAALER B. A STABLE SUBSTRATE FOR THE ASSAY OF PLASMA KININ-FORMING ENZYMES. Br J Pharmacol Chemother. 1963 Dec;21:500–508. doi: 10.1111/j.1476-5381.1963.tb02018.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beilenson S., Schachter M., Smaje L. H. Secretion of kallikrein and its role in vasodilatation in the submaxillary gland. J Physiol. 1968 Dec;199(2):303–317. doi: 10.1113/jphysiol.1968.sp008655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhoola K. D., Morley J., Schachter M., Smaje L. H. Vasodilatation in the submaxillary gland of the cat. J Physiol. 1965 Jul;179(1):172–184. doi: 10.1113/jphysiol.1965.sp007656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Darke A. C., Smaje L. H. Dependence of functional vasodilatation in the cat submaxillary gland upon stimulation frequency. J Physiol. 1972 Oct;226(1):191–203. doi: 10.1113/jphysiol.1972.sp009980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Garrett J. R. The innervation of salivary glands. 3. The effects of certain experimental procedures on cholinesterase-positive nerves in glands of the cat. J R Microsc Soc. 1966 Oct;86(1):1–13. [PubMed] [Google Scholar]
  6. Garrett J. R. The innervation of salivary glands. IV. The effects of certain experimental procedures on the ultrastructure of nerves in glands of the cat. J R Microsc Soc. 1966 Oct;86(1):15–31. [PubMed] [Google Scholar]
  7. Gautvik K. M., Hilton S. M., Torres S. H. Consumption of kininogen in the submandibular salivary gland when activated by chorda stimulation. J Physiol. 1970 Nov;211(1):49–61. doi: 10.1113/jphysiol.1970.sp009265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gautvik K. Parasympathetic neuro-effector transmission and functional vasodilatation in the submandibular salivary gland of cats. Acta Physiol Scand. 1970 Jun;79(2):204–215. doi: 10.1111/j.1748-1716.1970.tb04720.x. [DOI] [PubMed] [Google Scholar]
  9. Gautvik K. The interaction of two different vasodilator mechanisms in the chorda-tympani activated submandibular salivary gland. Acta Physiol Scand. 1970 Jun;79(2):188–203. doi: 10.1111/j.1748-1716.1970.tb04719.x. [DOI] [PubMed] [Google Scholar]
  10. HILTON S. M., LEWIS G. P. The cause of the vasodilatation accompanying activity in the submandibular salivary gland. J Physiol. 1955 May 27;128(2):235–248. doi: 10.1113/jphysiol.1955.sp005302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HILTON S. M., LEWIS G. P. The mechanism of the functional hyperaemia in the submandibular salivary gland. J Physiol. 1955 Aug 29;129(2):253–271. doi: 10.1113/jphysiol.1955.sp005351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HILTON S. M., LEWIS G. P. The relationship between glandular activity, bradykinin formation and functional vasodilatation in the submandibular salivary gland. J Physiol. 1956 Nov 28;134(2):471–483. doi: 10.1113/jphysiol.1956.sp005658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hilton S. M., Torres S. H. Selective hypersensitivity to bradykinin in salivary glands with ligated ducts. J Physiol. 1970 Nov;211(1):37–48. doi: 10.1113/jphysiol.1970.sp009264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jewell B. R., Kretzschmar M., Woledge R. C. Length and tension transducers. J Physiol. 1967 Jul;191(1):10P–12P. [PubMed] [Google Scholar]
  15. Karpinski E., Barton S., Schachter M. Vasodilator nerve fibres to the submaxillary gland of the cat. Nature. 1971 Jul 9;232(5306):122–124. doi: 10.1038/232122a0. [DOI] [PubMed] [Google Scholar]
  16. Morley J., Schachter M., Smaje L. H. Vasodilatation in the submaxillary gland of the rabbit. J Physiol. 1966 Dec;187(3):595–602. doi: 10.1113/jphysiol.1966.sp008111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schachter M., Beilenson S. Mediator of vasodilatation in the submaxillary gland. Fed Proc. 1968 Jan-Feb;27(1):73–75. [PubMed] [Google Scholar]
  18. Skinner N. S., Jr, Webster M. E. Kinins, beta-adrenergic receptors and functional vasodilatation in the submaxillary gland of the cat. J Physiol. 1968 Apr;195(3):505–519. doi: 10.1113/jphysiol.1968.sp008471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. TERROUX K. G., SEKELJ P., BURGEN A. S. Oxygen consumption and blood flow in the submaxillary gland of the dog. Can J Biochem Physiol. 1959 Jan;37(1):5–15. [PubMed] [Google Scholar]
  20. WERLE E., TRAUTSCHOLD I. Kallikrein, kallidin, kallikrein inhibitors. Ann N Y Acad Sci. 1963 Feb 4;104:117–129. doi: 10.1111/j.1749-6632.1963.tb17657.x. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES