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. 1972 Jun;223(2):549–570. doi: 10.1113/jphysiol.1972.sp009862

Post-stimulus hyperpolarization and slow potassium conductance increase in Aplysia giant neurone

M S Brodwick, D Junge
PMCID: PMC1331462  PMID: 5039286

Abstract

1. Intracellular records from Aplysia giant (R2) cell somata showed long lasting 4-10 mV hyperpolarizations after passage of outward current through a second intracellular electrode.

2. An increase in membrane slope conductance occurred simultaneously with the post-stimulus hyperpolarization (PSH).

3. Both the PSH and conductance-increase varied strongly with stimulus amplitude and duration.

4. Both the PSH and the conductance increase occurred in Ca-free medium containing tetrodotoxin, when action-potential production was completely blocked.

5. The PSH persisted in the presence of ouabain or DNP, with cooling, with removal of external K+, and in media where all the Na+ was replaced with Li+, suggesting that it was not due to the activity of an electrogenic pump.

6. A reversal potential for the PSH was demonstrated by application of maintained inward current following the end of an outward-directed stimulus.

7. The PSH reversal potential varied with [K]o, but not with [Cl]o or [Na]o, suggesting that the PSH was mainly due to an increase in K conductance.

8. The PSH and the conductance increase were reduced strongly when all the Na+ was replaced with Tris, and only slightly when Na+ was replaced with sucrose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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