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. 1972 Feb;220(3):565–582. doi: 10.1113/jphysiol.1972.sp009723

Activation of the electrogenic sodium pump in guinea-pig auricles by internal sodium ions

H G Glitsch
PMCID: PMC1331670  PMID: 5016038

Abstract

1. The effect of various intracellular Na concentrations ([Na]i) on the membrane potential after hypothermia was studied in guinea-pig auricles.

2. For varying [Na]i, the atria were cooled for 4 hr at 4-6° C in a K-poor solution with different concentrations of NaCl. The auricles were rewarmed in normal Tyrode solution at 35° C.

3. Extracellular space (ECS), intracellular Na and K concentrations ([Na]i and [K]i) and membrane potential of the atria were measured before and after hypothermia.

4. The ECS, measured as inulin space, amounted to 350 ml./kg wet wt. at 35° C and to 300 ml./kg wet wt. at 4-6° C.

5. [K]i decreased during cooling and increased during rewarming the auricles. [Na]i increased during hypothermia in bathing fluids containing NaCl, but decreased in NaCl- and Na-free solutions. At the beginning of rewarming a net Na transport occurred from cells with high [Na]i, while a net Na uptake took place in atria with low [Na]i.

6. At the same time, the membrane potential of auricles with increased [Na]i hyperpolarized beyond the steady-state value recorded at the end of rewarming, or even beyond the calculated K+ equilibrium potential (EK). Afterwards, the hyperpolarization levelled off, while the EK values increased further. The membrane potential of atria with decreased [Na]i showed no transitory hyperpolarization during rewarming.

7. The hyperpolarization beyond the steady-state value of membrane potential in rewarmed auricles was significantly correlated to the active Na efflux.

8. From these results it is concluded that the membrane potential of guinea-pig atria after hypothermia is affected by an active, electrogenic Na pump activated by intracellular Na ions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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