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. 1971 Mar;213(3):581–598. doi: 10.1113/jphysiol.1971.sp009402

The effect of ACTH on calcium distribution in the perfused cat adrenal gland

Siret D Jaanus, R P Rubin
PMCID: PMC1331743  PMID: 4323934

Abstract

1. Experiments were carried out to study the effects of adrenocorticotrophin (ACTH) on calcium distribution in the isolated cat adrenal gland perfused with Locke solution.

2. The addition of ACTH to Locke solution caused a small, but significant, increase in the radiocalcium (45Ca) space and content of the adrenal cortex.

3. The average 45Ca washout curve obtained after exposure to ACTH was significantly displaced above the average washout curve for the control glands during the first 20 min of washout. During the time that the two curves were significantly different, maximum corticosteroid secretion was attained.

4. The rate of 45Ca efflux was slowed after ACTH was added to the perfusion medium.

5. The total calcium content of the cortex was not altered by ACTH.

6. Perfusion with calcium-free Locke caused a rapid decrease in the calcium content of the cortex to about 20% of the control value. Dinitrophenol was required to deplete completely the cortical calcium content during perfusion with the calcium-deprived medium.

7. The results are consistent with the suggestion that a translocation of calcium occurs during stimulation of the cortex by ACTH. The source of the calcium ions needed to support the secretory response to ACTH is probably not the extracellular fluid; but ACTH may shift calcium from a rapidly exchanging to a more slowly exchanging cellular pool.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOHR D. F. ELECTROLYTES AND SMOOTH MUSCLE CONTRACTION. Pharmacol Rev. 1964 Mar;16:85–127. [PubMed] [Google Scholar]
  2. Carafoli E. In vivo effect of uncoupling agents on the incorporation of calcium and strontium into mitochondria and other subcellular fractions of rat liver. J Gen Physiol. 1967 Aug;50(7):1849–1864. doi: 10.1085/jgp.50.7.1849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DOUGLAS W. W., POISNER A. M. CALCIUM MOVEMENT IN THE NEUROHYPOPHYSIS OF THE RAT AND ITS RELATION TO THE RELEASE OF VASOPRESSIN. J Physiol. 1964 Jul;172:19–30. doi: 10.1113/jphysiol.1964.sp007400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DOUGLAS W. W., POISNER A. M. On the mode of action of acetylcholine in evoking adrenal medullary secretion: increased uptake of calcium during the secretory response. J Physiol. 1962 Aug;162:385–392. doi: 10.1113/jphysiol.1962.sp006940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DOUGLAS W. W., RUBIN R. P. The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J Physiol. 1961 Nov;159:40–57. doi: 10.1113/jphysiol.1961.sp006791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Douglas W. W., Kanno T., Sampson S. R. Influence of the ionic environment on the membrane potential of adrenal chromaffin cells and on the depolarizing effect of acetylcholine. J Physiol. 1967 Jul;191(1):107–121. doi: 10.1113/jphysiol.1967.sp008239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Douglas W. W., Poisner A. M., Rubin R. P. Efflux of adenine nucleotides from perfused adrenal glands exposed to nicotine and other chromaffin cell stimulants. J Physiol. 1965 Jul;179(1):130–137. doi: 10.1113/jphysiol.1965.sp007652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Douglas W. W. Stimulus-secretion coupling: the concept and clues from chromaffin and other cells. Br J Pharmacol. 1968 Nov;34(3):451–474. doi: 10.1111/j.1476-5381.1968.tb08474.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fawcett D. W., Long J. A., Jones A. L. The ultrastructure of endocrine glands. Recent Prog Horm Res. 1969;25:315–380. doi: 10.1016/b978-0-12-571125-8.50010-7. [DOI] [PubMed] [Google Scholar]
  10. HODGKIN A. L., KEYNES R. D. Movements of labelled calcium in squid giant axons. J Physiol. 1957 Sep 30;138(2):253–281. doi: 10.1113/jphysiol.1957.sp005850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hales C. N., Milner R. D. Cations and the secretion of insulin from rabbit pancreas in vitro. J Physiol. 1968 Nov;199(1):177–187. doi: 10.1113/jphysiol.1968.sp008647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Harvey A. M., Macintosh F. C. Calcium and synaptic transmission in a sympathetic ganglion. J Physiol. 1940 Jan 15;97(3):408–416. doi: 10.1113/jphysiol.1940.sp003818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holzbauer M., Newport H. M. Adrenal secretion rates and adrenal tissue concentrations of pregnenolone, progesterone, 11 beta OH-androstenedione and some other steroids in young pigs and dogs. J Physiol. 1969 Feb;200(3):821–848. doi: 10.1113/jphysiol.1969.sp008724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ishida A. The effect of tetrodotoxin on the calcium-dependent link in stimulus-secretion coupling in neurohypophysis. Jpn J Physiol. 1967 Jun;17(3):308–320. doi: 10.2170/jjphysiol.17.308. [DOI] [PubMed] [Google Scholar]
  15. Jaanus S. D., Rosenstein M. J., Rubin R. P. On the mode of action of ACTH on the isolated perfused adrenal gland. J Physiol. 1970 Aug;209(3):539–556. doi: 10.1113/jphysiol.1970.sp009178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Katz B., Miledi R. The timing of calcium action during neuromuscular transmission. J Physiol. 1967 Apr;189(3):535–544. doi: 10.1113/jphysiol.1967.sp008183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kirshner N., Sage H. J., Smith W. J. Mechanism of secretion from the adrenal medulla. II. Release of catecholamines and storage vesicle protein in response to chemical stimulation. Mol Pharmacol. 1967 May;3(3):254–265. [PubMed] [Google Scholar]
  18. Lacy P. E., Howell S. L., Young D. A., Fink C. J. New hypothesis of insulin secretion. Nature. 1968 Sep 14;219(5159):1177–1179. doi: 10.1038/2191177a0. [DOI] [PubMed] [Google Scholar]
  19. Lehninger A. L., Carafoli E., Rossi C. S. Energy-linked ion movements in mitochondrial systems. Adv Enzymol Relat Areas Mol Biol. 1967;29:259–320. doi: 10.1002/9780470122747.ch6. [DOI] [PubMed] [Google Scholar]
  20. Long J. A., Jones A. L. The fine structure of the zona glomerulosa and the zona fasciculata of the adrenal cortex of the opossum. Am J Anat. 1967 May;120(3):463–487. doi: 10.1002/aja.1001200305. [DOI] [PubMed] [Google Scholar]
  21. MONGAR J. L., SCHILD H. O. The effect of calcium and pH on the anaphylactic reaction. J Physiol. 1958 Feb 17;140(2):272–284. doi: 10.1113/jphysiol.1958.sp005933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Matthews E. K. Membrane potential measurement in cells of the adrenal gland. J Physiol. 1967 Mar;189(1):139–148. doi: 10.1113/jphysiol.1967.sp008159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Matthews E. K., Saffran M. Steroid production and membrane potential measurement in cells of the adrenal cortex. J Physiol. 1967 Mar;189(1):149–161. doi: 10.1113/jphysiol.1967.sp008160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Padawer J. Uptake of colloidal thorium dioxide by mast cells. J Cell Biol. 1969 Mar;40(3):747–760. doi: 10.1083/jcb.40.3.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rubin R. P., Feinstein M. B., Jaanus S. D., Paimre M. Inhibition of catecholamine secretion and calcium exchange in perfused cat adrenal glands by tetracaine and magnesium. J Pharmacol Exp Ther. 1967 Mar;155(3):463–471. [PubMed] [Google Scholar]
  26. Rubin R. P. The role of calcium in the release of neurotransmitter substances and hormones. Pharmacol Rev. 1970 Sep;22(3):389–428. [PubMed] [Google Scholar]
  27. SHANES A. M., BIANCHI C. P. The distribution and kinetics of release of radiocalcium in tendon and skeletal muscle. J Gen Physiol. 1959 May 20;42(5):1123–1137. doi: 10.1085/jgp.42.5.1123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schneider F. H., Smith A. D., Winkler H. Secretion from the adrenal medulla: biochemical evidence for exocytosis. Br J Pharmacol Chemother. 1967 Sep;31(1):94–104. doi: 10.1111/j.1476-5381.1967.tb01980.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Vogt M. The output of cortical hormone by the mammalian suprarenal. J Physiol. 1943 Dec 31;102(3):341–356. doi: 10.1113/jphysiol.1943.sp004041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Whittaker V. P. Structure and function of animal-cell membranes. Br Med Bull. 1968 May;24(2):101–106. doi: 10.1093/oxfordjournals.bmb.a070608. [DOI] [PubMed] [Google Scholar]
  31. von Hattingberg H. M., Klaus W., Lüllmann H., Zepf S. Fluorometrische Bestimmung von Mikromengen Calcium in Muskelgewebe. Experientia. 1966 Aug 15;22(8):553–555. doi: 10.1007/BF01898689. [DOI] [PubMed] [Google Scholar]

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