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. 1975 Dec;253(2):459–475. doi: 10.1113/jphysiol.1975.sp011200

Retention and redistribution of proteins in mammalian nerve fibres by axoplasmic transport.

S Ochs
PMCID: PMC1348516  PMID: 55480

Abstract

Fast axoplasmic transport is characterized by a crest of labelled activity moving down nerve fibres after injection of the L7 dorsal root ganglion with the amino acid precursor (3H) leucine, the crest followed by a plateau which represents in part a later egress of labelled components from compartments in the cell bodies and in part materials left behind the advancing crest. 2. after making ligations just below the ganglia at different times after injection of the precursor, a small downward slope of locally retained activity of incorporated materials is seen in the plateau remaining in the nerves. The slope becomes changed to a horizontal level when in addition a distal ligation is made as a result of the redistribution of labelled materials within the doubly ligated nerve segments. 3. the outlfow pattern at later times, at a day and longer after injection, shows an additional spread of activity from the cell body region. The pattern of outflow gradually levels off at later times as additions of activity are made to the more distal part of the nerves. The activity retained in the nerves becomes less free to become redistributed in the course of several days. 4. The temporal changes in the outflow patterns can be accounted for by the local retention and redistribution of the labelled materials within the fibres. Later additions of labelled materials compartmented in the cell bodies also contribute to the later pattern of outflow. A "unitary" view for fast and slow transport is presented based on the transport filament hypothesis earlier proposed to account for fast axoplasmic transport.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dahlström A. Axoplasmic transport (with particular respect to adrenergic neurons). Philos Trans R Soc Lond B Biol Sci. 1971 Jun 17;261(839):325–358. doi: 10.1098/rstb.1971.0064. [DOI] [PubMed] [Google Scholar]
  2. Fink B. R., Kennedy R. D., Hendrickson A. E., Middaugh M. E. Lidocaine inhibition of rapid axonal transport. Anesthesiology. 1972 May;36(5):422–432. doi: 10.1097/00000542-197205000-00002. [DOI] [PubMed] [Google Scholar]
  3. Gross G. W., Beidler L. M. A quantitative analysis of isotope concentration profiles and rapid transport velocities in the C-fibers of the garfish olfactory nerve. J Neurobiol. 1975 Mar;6(2):213–232. doi: 10.1002/neu.480060208. [DOI] [PubMed] [Google Scholar]
  4. Gross G. W., Beidler L. M. Fast axonal transport in the c-fibers of the garfish olfactory nerve. J Neurobiol. 1973;4(5):413–428. doi: 10.1002/neu.480040503. [DOI] [PubMed] [Google Scholar]
  5. Heslop J. P. Axonal flow and fast transport in nerves. Adv Comp Physiol Biochem. 1975;6:75–163. doi: 10.1016/b978-0-12-011506-8.50008-1. [DOI] [PubMed] [Google Scholar]
  6. Jeffrey P. L., Austin L. Axoplasmic transport. Prog Neurobiol. 1973;2(3):207–255. [PubMed] [Google Scholar]
  7. Karlsson J. O., Sjöstrand J. Synthesis, migration and turnover of protein in retinal ganglion cells. J Neurochem. 1971 May;18(5):749–767. doi: 10.1111/j.1471-4159.1971.tb12005.x. [DOI] [PubMed] [Google Scholar]
  8. Kidwai A. M., Ochs S. Components of fast and slow phases of axoplasmic flow. J Neurochem. 1969 Jul;16(7):1105–1112. doi: 10.1111/j.1471-4159.1969.tb05955.x. [DOI] [PubMed] [Google Scholar]
  9. Kristensson K., Olsson Y. Uptake and retrograde axonal transport of peroxidase in hypoglossal neurons. Electron microscopical localization in the neuronal perikaryon. Acta Neuropathol. 1971;19(1):1–9. doi: 10.1007/BF00690948. [DOI] [PubMed] [Google Scholar]
  10. LaVail J. H., LaVail M. M. Retrograde axonal transport in the central nervous system. Science. 1972 Jun 30;176(4042):1416–1417. doi: 10.1126/science.176.4042.1416. [DOI] [PubMed] [Google Scholar]
  11. Lasek R. Axoplasmic transport in cat dorsal root ganglion cells: as studied with [3-H]-L-leucine. Brain Res. 1968 Mar;7(3):360–377. doi: 10.1016/0006-8993(68)90003-6. [DOI] [PubMed] [Google Scholar]
  12. Lubińska L., Niemierko S. Velocity and intensity of bidirectional migration of acetylcholinesterase in transected nerves. Brain Res. 1971 Apr 2;27(2):329–342. doi: 10.1016/0006-8993(71)90258-7. [DOI] [PubMed] [Google Scholar]
  13. MIANI N. ANALYSIS OF THE SOMATO-AXONAL MOVEMENT OF PHOSPHOLIPIDS IN THE VAGUS AND HYPOGLOSSAL NERVES. J Neurochem. 1963 Dec;10:859–874. doi: 10.1111/j.1471-4159.1963.tb11913.x. [DOI] [PubMed] [Google Scholar]
  14. Martinez A. J., Friede R. L. Accumulation of axoplasmic organelles in swollen nerve fibers. Brain Res. 1970 Apr 14;19(2):183–198. doi: 10.1016/0006-8993(70)90433-6. [DOI] [PubMed] [Google Scholar]
  15. OCHS S., DALRYMPLE D., RICHARDS G. Axoplasmic flow in ventral root nerve fibers of the cat. Exp Neurol. 1962 May;5:349–363. doi: 10.1016/0014-4886(62)90049-3. [DOI] [PubMed] [Google Scholar]
  16. Ochs S. Fast transport of materials in mammalian nerve fibers. Science. 1972 Apr 21;176(4032):252–260. doi: 10.1126/science.176.4032.252. [DOI] [PubMed] [Google Scholar]
  17. Ochs S., Hollingsworth D. Dependence of fast axoplasmic transport in nerve on oxidative metabolism. J Neurochem. 1971 Jan;18(1):107–114. doi: 10.1111/j.1471-4159.1971.tb00172.x. [DOI] [PubMed] [Google Scholar]
  18. Ochs S., Johnson J. Fast and slow phases of axoplasmic flow in ventral root nerve fibres. J Neurochem. 1969 Jun;16(3):845–853. doi: 10.1111/j.1471-4159.1969.tb08972.x. [DOI] [PubMed] [Google Scholar]
  19. Ochs S., Ranish N. Characteristics of the fast transport system in mammalian nerve fibers. J Neurobiol. 1969;1(2):247–261. doi: 10.1002/neu.480010211. [DOI] [PubMed] [Google Scholar]
  20. Ochs S. Rate of fast axoplasmic transport in mammalian nerve fibres. J Physiol. 1972 Dec;227(3):627–645. doi: 10.1113/jphysiol.1972.sp010051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ranish N., Ochs S. Fast axoplasmic transport of acetylcholinesterase in mammalian nerve fibres. J Neurochem. 1972 Nov;19(11):2641–2649. doi: 10.1111/j.1471-4159.1972.tb01323.x. [DOI] [PubMed] [Google Scholar]
  22. Sabri M. I., Ochs S. Characterization of fast and slow transported proteins in dorsal root and sciatic nerve of cat. J Neurobiol. 1973;4(2):145–165. doi: 10.1002/neu.480040206. [DOI] [PubMed] [Google Scholar]
  23. Spencer P. S. Reappraisal of the model for "bulk axoplasmic flow". Nat New Biol. 1972 Dec 27;240(104):283–285. doi: 10.1038/newbio240283a0. [DOI] [PubMed] [Google Scholar]

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