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. 1970 Jun;208(2):299–315. doi: 10.1113/jphysiol.1970.sp009121

The half-life of exogenous gastrin in the circulation

E L Blair, Yvonne Farra, Diana D Richardson, P Steinbok
PMCID: PMC1348750  PMID: 5500725

Abstract

1. A method of gastrin bio-assay is described which can be used on as little as 30 ng synthetic human gastrin I at a minimum concentration of 2·5 ng/ml.

2. Pentagastrin or synthetic human gastrin I added to cat plasma can be stored on ice or at 4° C, for periods up to 27 hr without apparent loss of gastrin activity.

3. Between 1½ and 13 min after the rapid I.V. injection of pentagastrin in the anaesthetized cat and between 1½ and 15 min after the injection of synthetic human gastrin I, there is a rapid reduction of the gastrin concentration in the arterial plasma. The data relating log10 gastrin concentration in arterial plasma with time can be fitted by a single term.

4. Studies in vitro show that over the periods of time involved in the in vivo studies, both pentagastrin and synthetic human gastrin I are stable in cat plasma at 37° C in concentrations which occurred in the circulating plasma.

5. The half-life of pentagastrin in the circulating arterial plasma of the anaesthetized cat is 1·50 min (S.E. ± 0·08) and the half-life of synthetic human gastrin I is 2·65 min (S.E. ± 0·09).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERSON S. A., YALOW R. S., VOLK B. W. In vivo and in vitro metabolism of insulin-I 131 and glucagon-I 131 in normal and cortisone-treated rabbits. J Lab Clin Med. 1957 Mar;49(3):331–342. [PubMed] [Google Scholar]
  2. Blair E. L., Keenlyside R. M., Newell D. J., Reed J. D., Richardson D. D. Assay of gastrin by means of its gastric acid stimulating activity. J Physiol. 1968 Oct;198(3):613–626. doi: 10.1113/jphysiol.1968.sp008629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHAUDHURY R. R., WALKER J. M. The fate of injected oxytocin in the rabbit. J Endocrinol. 1959 Nov;19:189–192. doi: 10.1677/joe.0.0190189. [DOI] [PubMed] [Google Scholar]
  4. DICKER S. E., NUNN J. Fate and excretion of the pressor activity of vasopressin in rats. J Physiol. 1957 Aug 29;138(1):11–18. doi: 10.1113/jphysiol.1957.sp005834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EDELMAN I. S. Exchange of water between blood and tissues; characteristics of deuterium oxidases equilibration in body water. Am J Physiol. 1952 Nov;171(2):279–296. doi: 10.1152/ajplegacy.1952.171.2.279. [DOI] [PubMed] [Google Scholar]
  6. EGGLETON M. G. The state of body water in the cat. J Physiol. 1951 Dec 28;115(4):482–487. doi: 10.1113/jphysiol.1951.sp004683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GINSBURG M., HELLER H. Antidiuretic activity in blood obtained from various parts of the cardiovascular system. J Endocrinol. 1953 Jul;9(3):274–282. doi: 10.1677/joe.0.0090274. [DOI] [PubMed] [Google Scholar]
  8. GINSBURG M., HELLER H. The clearance of injected vasopressin from the circulation and its fate in the body. J Endocrinol. 1953 Jul;9(3):283–291. doi: 10.1677/joe.0.0090283. [DOI] [PubMed] [Google Scholar]
  9. GINSBURG M., SMITH M. W. The fate of oxytocin in male and female rats. Br J Pharmacol Chemother. 1959 Sep;14:327–333. doi: 10.1111/j.1476-5381.1959.tb00252.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GREENSPAN F. S., LI C. H., EVANS H. M. Disappearance rate of adrenocorticotropic hormone from rat's plasma after intravenous injection. Endocrinology. 1950 Mar;46(3):261–264. doi: 10.1210/endo-46-3-261. [DOI] [PubMed] [Google Scholar]
  11. Greengard H., Grossman M. I., Woolley J. R., Ivy A. C. A CONFIRMATION OF THE PRESENCE OF PANCREOZYMIN IN THE DUODENAL MUCOSA. Science. 1944 Apr 28;99(2574):350–351. doi: 10.1126/science.99.2574.350. [DOI] [PubMed] [Google Scholar]
  12. INGBAR S. H., FREINKEL N. Regulation of the peripheral metabolism of the thyroid hormones. Recent Prog Horm Res. 1960;16:353–403. [PubMed] [Google Scholar]
  13. JACKSON R. H., BLAIR E. L., DAWSON P. J., REED J. D., WATTS W. P. GASTRIN ACTIVITY OF TUMOUR TISSUEIN A CHILD WITH THE ZOLLINGER-ELLISON SYNDROME. Lancet. 1963 Nov 2;2(7314):908–912. doi: 10.1016/s0140-6736(63)90619-6. [DOI] [PubMed] [Google Scholar]
  14. LAUSON H. D., BOCANEGRA M. Clearance of exogenous vasopressin from plasma of dogs. Am J Physiol. 1961 Mar;200:493–497. doi: 10.1152/ajplegacy.1961.200.3.493. [DOI] [PubMed] [Google Scholar]
  15. Laster L., Walsh J. H. Enzymatic degradation of C-terminal tetrapeptide amide of gastrin by mammalian tissue extracts. Fed Proc. 1968 Nov-Dec;27(6):1328–1330. [PubMed] [Google Scholar]
  16. SHARE L. Rate of disappearance of arginine vasopressin from circulating blood in the dog. Am J Physiol. 1962 Dec;203:1179–1181. doi: 10.1152/ajplegacy.1962.203.6.1179. [DOI] [PubMed] [Google Scholar]
  17. SILVER L., SCHWARTZ I. L., FONG C. T., DEBONS A. F., DAHL L. K. Disappearance of plasma radioactivity after injection of H3- or I-131-labeled arginine vasopressin. J Appl Physiol. 1961 Nov;16:1097–1099. doi: 10.1152/jappl.1961.16.6.1097. [DOI] [PubMed] [Google Scholar]
  18. THORN N. A. Mammalian antidiuretic hormone. Physiol Rev. 1958 Apr;38(2):169–195. doi: 10.1152/physrev.1958.38.2.169. [DOI] [PubMed] [Google Scholar]
  19. THORN N. A., SILVER L. Chemical form of circulating antidiuretic hormone in rats. J Exp Med. 1957 Jun 1;105(6):575–583. doi: 10.1084/jem.105.6.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. UNGER R. H., EISENTRAUT A. M., McCALL M. S., MADISON L. L. Glucagon antibodies and an immunoassay for glucagon. J Clin Invest. 1961 Jul;40:1280–1289. doi: 10.1172/JCI104357. [DOI] [PMC free article] [PubMed] [Google Scholar]

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