Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1973 Jul;232(1):71–85. doi: 10.1113/jphysiol.1973.sp010257

Effects of biogenic amines on central thermoresponsive neurones in the rabbit

T Hori, T Nakayama
PMCID: PMC1350492  PMID: 4733509

Abstract

1. Single unit activities were recorded with five-barrelled micropipettes from the thermo-responsive neurones in the preoptic area and the mid-brain reticular formation in urethanized rabbits. 5-hydroxytryptamine (5-HT), noradrenaline (NA) and acetylcholine (ACh) were applied micro-iontophoretically to the immediate vicinity of the recording cells.

2. Out of seventeen warm-responsive neurones recorded in the preoptic area, fifteen neurones responded to 5-HT with the increase in firing rate and two showed no response. Thirteen out of seventeen warm-units decreased their firing rate in response to application of NA and four were not affected. ACh had no effect on any of the warm-units examined.

3. Six out of seven cold-units in the preoptic area were depressed by 5-HT, while NA excited five of six units studied. None of the cold-units were influenced by ACh.

4. These results are in good agreement with the changes in rectal temperature produced by 5-HT and NA micro-injected into the hypothalamus in rabbits.

5. In the mid-brain reticular formation, 5-HT excited all of fourteen cold-responsive neurones. Of these, eight cold-units were depressed and six were unaffected by NA, while ACh excited six units and had no effect on eight units. All of the five warm-responsive units were inhibited by 5-HT and none were influenced by NA. Thus, the responses of reticular thermoresponsive neurones to 5-HT and NA were opposite to those of the preoptic thermo-responsive neurones.

Full text

PDF
85

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOGDANSKI D. F., WEISSBACH H., UDENFRIEND S. The distribution of serotonin, 5-hydroxytryptophan decarboxylase, and monoamine oxidase in brain. J Neurochem. 1957;1(3):272–278. doi: 10.1111/j.1471-4159.1957.tb12082.x. [DOI] [PubMed] [Google Scholar]
  2. Beckman A. L., Carlisle H. J. Effect of intrahypothalamic infusion of acetylcholine on behavioural and physiological thermoregulation in the rat. Nature. 1969 Feb 8;221(5180):561–562. doi: 10.1038/221561a0. [DOI] [PubMed] [Google Scholar]
  3. Beckman A. L. Effect of intrahypothalamic norepinephrine on thermoregulatory responses in the rat. Am J Physiol. 1970 Jun;218(6):1596–1604. doi: 10.1152/ajplegacy.1970.218.6.1596. [DOI] [PubMed] [Google Scholar]
  4. Beckman A. L., Eisenman J. S. Microelectrophoresis of biogenic amines on hypothalamic thermosensitive cells. Science. 1970 Oct 16;170(3955):334–336. doi: 10.1126/science.170.3955.334. [DOI] [PubMed] [Google Scholar]
  5. Bradley P. B., Candy J. M. Iontophoretic release of acetylcholine, noradrenaline, 5-hydroxytryptamine and D-lysergic acid diethylamide from micropipettes. Br J Pharmacol. 1970 Oct;40(2):194–201. doi: 10.1111/j.1476-5381.1970.tb09913.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bradley P. B., Dhawan B. N., Wolstencroft J. H. Pharmacological properties of cholinoceptive neurones in the medulla and pons of the cat. J Physiol. 1966 Apr;183(3):658–674. doi: 10.1113/jphysiol.1966.sp007891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cabanac M., Stolwijk J. A., Hardy J. D. Effect of temperature and pyrogens on single-unit activity in the rabbit's brain stem. J Appl Physiol. 1968 May;24(5):645–652. doi: 10.1152/jappl.1968.24.5.645. [DOI] [PubMed] [Google Scholar]
  8. Cooper K. E., Cranston W. I., Honour A. J. Effects of intraventricular and intrahypothalamic injection of noradrenaline and 5-HT on body temperature in conscious rabbits. J Physiol. 1965 Dec;181(4):852–864. doi: 10.1113/jphysiol.1965.sp007801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Corrodi H., Fuxe K., Hökfelt T. A possible role played by central monoamine neurones in thermo-regulation. Acta Physiol Scand. 1967 Oct-Nov;71(2):224–232. doi: 10.1111/j.1748-1716.1967.tb03728.x. [DOI] [PubMed] [Google Scholar]
  10. Cranston W. I., Hellon R. F., Luff R. H., Rawlins M. D. Hypothalamic endogenous noradrenaline and thermoregulation in the cat and rabbit. J Physiol. 1972 May;223(1):59–67. doi: 10.1113/jphysiol.1972.sp009834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cunningham D. J., Stolwijk J. A., Murakami N., Hardy J. D. Responses of neurons in the preoptic area to temperature, serotonin, and epinephrine. Am J Physiol. 1967 Dec;213(6):1570–1581. doi: 10.1152/ajplegacy.1967.213.6.1570. [DOI] [PubMed] [Google Scholar]
  12. Eisenman J. S., Jackson D. C. Thermal response patterns of septal and preoptic neurons in cats. Exp Neurol. 1967 Sep;19(1):33–45. doi: 10.1016/0014-4886(67)90005-2. [DOI] [PubMed] [Google Scholar]
  13. Eisenman J. S. Pyrogen-induced changes in the thermosensitivity of septal and preoptic neurons. Am J Physiol. 1969 Feb;216(2):330–334. doi: 10.1152/ajplegacy.1969.216.2.330. [DOI] [PubMed] [Google Scholar]
  14. FELDBERG W., MYERS R. D. A NEW CONCEPT OF TEMPERATURE REGULATION BY AMINES IN THE HYPOTHALAMUS. Nature. 1963 Dec 28;200:1325–1325. doi: 10.1038/2001325a0. [DOI] [PubMed] [Google Scholar]
  15. FELDBERG W., MYERS R. D. CHANGES IN TEMPERATURE PRODUCED BY MICRO-INJECTIONS OF AMINES INTO THE ANTERIOR HYPOTHALAMUS OF CATS. J Physiol. 1965 Mar;177:239–245. doi: 10.1113/jphysiol.1965.sp007589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. FELDBERG W., MYERS R. D. EFFECTS ON TEMPERATURE OF AMINES INJECTED INTO THE CEREBRAL VENTRICLES. A NEW CONCEPT OF TEMPERATURE REGULATION. J Physiol. 1964 Sep;173:226–231. doi: 10.1113/jphysiol.1964.sp007454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Feldberg W., Hellon R. F., Lotti V. J. Temperature effects produced in dogs and monkeys by injections of monoamines and related substances into the third ventricle. J Physiol. 1967 Aug;191(3):501–515. doi: 10.1113/jphysiol.1967.sp008264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Feldberg W., Lotti V. J. Temperature responses to monoamines and an inhibitor of MAO injected into the cerebral ventricles of rats. Br J Pharmacol Chemother. 1967 Sep;31(1):152–161. doi: 10.1111/j.1476-5381.1967.tb01985.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. HARDY J. D., HELLON R. F., SUTHERLAND K. TEMPERATURE-SENSITIVE NEURONES IN THE DOG'S HYPOTHALAMUS. J Physiol. 1964 Dec;175:242–253. doi: 10.1113/jphysiol.1964.sp007515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hassler C. R., McCook R. D. Hypothalamic blood flow in cats during injection of biogenic amines. Am J Physiol. 1971 Jan;220(1):196–201. doi: 10.1152/ajplegacy.1971.220.1.196. [DOI] [PubMed] [Google Scholar]
  21. Hellon R. F. Thermal stimulation of hypothalamic neurones in unanaesthetized rabbits. J Physiol. 1967 Nov;193(2):381–395. doi: 10.1113/jphysiol.1967.sp008364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lomax P., Foster R. S., Kirkpatrick W. E. Cholinergic and adrenergic interactions in the thermoregulatory centers of the rat. Brain Res. 1969 Oct;15(2):431–438. doi: 10.1016/0006-8993(69)90165-6. [DOI] [PubMed] [Google Scholar]
  23. Myers R. D., Beleslin D. B. Changes in serotonin release in hypothalamus during cooling or warming of the monkey. Am J Physiol. 1971 Jun;220(6):1746–1754. doi: 10.1152/ajplegacy.1971.220.6.1746. [DOI] [PubMed] [Google Scholar]
  24. Myers R. D., Yaksh T. L. Control of body temperature in the unanaesthetized monkey by cholinergic and aminergic systems in the hypothalamus. J Physiol. 1969 Jun;202(2):483–500. doi: 10.1113/jphysiol.1969.sp008822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Nakayama T., Hardy J. D. Unit responses in the rabbit's brain stem to changes in brain and cutaneous temperature. J Appl Physiol. 1969 Dec;27(6):848–857. doi: 10.1152/jappl.1969.27.6.848. [DOI] [PubMed] [Google Scholar]
  26. Nakayama T., Hori T. Effects of anesthetic and pyrogen on thermally sensitive neurons in the brainstem. J Appl Physiol. 1973 Mar;34(3):351–355. doi: 10.1152/jappl.1973.34.3.351. [DOI] [PubMed] [Google Scholar]
  27. Rosendorff C., Mooney J. J. Central nervous system sites of action of a purified leucocyte pyrogen. Am J Physiol. 1971 Mar;220(3):597–603. doi: 10.1152/ajplegacy.1971.220.3.597. [DOI] [PubMed] [Google Scholar]
  28. SAWYER C. H., EVERETT J. W., GREEN J. D. The rabbit diencephalon in stereotaxic coordinates. J Comp Neurol. 1954 Dec;101(3):801–824. doi: 10.1002/cne.901010307. [DOI] [PubMed] [Google Scholar]
  29. UDENFRIEND S., CREVELING C. R. Localization of dopamine-beta-oxidase in brain. J Neurochem. 1959 Oct;4:350–352. doi: 10.1111/j.1471-4159.1959.tb13216.x. [DOI] [PubMed] [Google Scholar]
  30. VOGT M. The concentration of sympathin in different parts of the central nervous system under normal conditions and after the administration of drugs. J Physiol. 1954 Mar 29;123(3):451–481. doi: 10.1113/jphysiol.1954.sp005064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wit A., Wang S. C. Temperature-sensitive neurons in preoptic-anterior hypothalamic region: actions of pyrogen and acetylsalicylate. Am J Physiol. 1968 Nov;215(5):1160–1169. doi: 10.1152/ajplegacy.1968.215.5.1160. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES