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. 1969 Jul;203(1):93–109. doi: 10.1113/jphysiol.1969.sp008852

The respiratory efficiency and flexibility of erythrocytes stored in acid-citrate-dextrose solution

John A Sirs
PMCID: PMC1351516  PMID: 5821931

Abstract

1. Measurements have been made of the flexibility and respiratory efficiency of erythrocytes stored in acid-citrate-dextrose (ACD).

2. The rate of packing of stored blood, during centrifugation, indicates that the red blood cells are relatively inflexible.

3. Spectrophotometric observations, using the rapid-mixing and stopped-flow technique, indicate that the rate of egress of oxygen from HbO2 in these erythrocytes is significantly reduced. This is not due to a change of the chemical rate of dissociation of HbO2.

4. Neither factor is significantly reversed by resuspending the cells in Ringer-Locke solution or adjusting to pH 7·4.

5. A small improvement is obtained by adding hypertonic NaCl or incubating the blood at 37° C for 1 hr.

6. Incubation with adenosine, 25 μ-mole/ml. blood, at 37° C for at least 1 hr, restored both the respiratory function and flexibility to normal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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