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. 1966 Jan;182(1):57–65. doi: 10.1113/jphysiol.1966.sp007808

The release of 5-hydroxytryptamine from the rat stomach in vitro

A Bennett, Anne Bucknell, A C B Dean
PMCID: PMC1357455  PMID: 5937416

Abstract

1. 5-HT was released into the lumen of the intact isolated rat stomach and into the bath fluid surrounding a preparation of the body and antrum stretched mechanically.

2. Release of 5-HT increased when the pressure inside the intact stomach was raised or when the body/antrum preparation was stretched.

3. This increased release was not prevented by hexamethonium, atropine, hyoscine or procaine, and was probably due to distortion of cells containing 5-HT.

4. During periods of peristalsis induced by transmural stimulation the pharmacological activity of the fluid in the stomach was usually increased owing to a greater release of 5-HT and also to the release of an unidentified substance.

5. In reserpine-treated rats, 5-HT was released into the stomach but transmural stimulation did not produce true peristalsis and only rhythmic contractions occurred.

6. Peristalsis was seldom reduced by methysergide, and it is concluded that 5-HT is not essential for gastric peristalsis in the rat.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armitage A. K., Dean A. C. Function of the pylorus and pyloric antrum in gastric emptying. Gut. 1963 Jun;4(2):174–178. doi: 10.1136/gut.4.2.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armitage A. K., Dean A. C. The effects of pressure and pharmacologically active substances on gastric peristalsis in a transmurally stimulated rat soomach-duodenum preparation. J Physiol. 1966 Jan;182(1):42–56. doi: 10.1113/jphysiol.1966.sp007807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BULBRING E., CREMA A. The action of 5-hydroxytryptamine, 5-hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea-pigs. J Physiol. 1959 Apr 23;146(1):29–53. doi: 10.1113/jphysiol.1959.sp006176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BULBRING E., LIN R. C. The effect of intraluminal application of 5-hydroxytryptamine and 5-hydroxytryptophan on peristalsis; the local production of 5-HT and its release in relation to intraluminal pressure and propulsive activity. J Physiol. 1958 Mar 11;140(3):381–407. [PMC free article] [PubMed] [Google Scholar]
  5. LEE C. Y. The effect of stimulation of extrinsic nerves on peristalsis and on the release of 5-hydroxytryptamine in the large intestine of the guinea-pig and of the rabbit. J Physiol. 1960 Jul;152:405–418. doi: 10.1113/jphysiol.1960.sp006496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. PATON W. D., VANE J. R. Analysis of he responses of the isolated stomach to electrical stimulation and to drugs. J Physiol. 1963 Jan;165:10–46. doi: 10.1113/jphysiol.1963.sp007040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SHORE P. A. Release of serotonin and catecholamines by drugs. Pharmacol Rev. 1962 Dec;14:531–550. [PubMed] [Google Scholar]
  8. VANE J. R. A sensitive method for the assay of 5-hydroxytryptamine. Br J Pharmacol Chemother. 1957 Sep;12(3):344–349. doi: 10.1111/j.1476-5381.1957.tb00146.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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