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. 1991 Apr;213(4):335–340. doi: 10.1097/00000658-199104000-00008

Biliary response to glucagon in humans.

G D Branum 1, B A Bowers 1, C R Watters 1, J Haebig 1, G Cucchiaro 1, M Farouk 1, W C Meyers 1
PMCID: PMC1358352  PMID: 2009015

Abstract

Glucagon has been demonstrated to have profound effect on biliary secretion in several species. Glucagon's biliary effects were studied in humans following biliary tract surgery. Nine patients underwent common bile duct exploration and insertion of a balloon-occludable t tube. An aliquot of the collected sample was kept and the enterohepatic circulation was maintained by reinfusion of the collected bile via the distal t-tube port. Glucagon increased bile flow and decreased cholesterol and phospholipid output during stable bile acid output. Furthermore high-performance liquid chromatographic analysis of bile acid profiles revealed no significant changes in bile salt species or conjugation after glucagon infusion. Glucagon is probably important in the physiologic regulation of biliary secretion in humans.

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Selected References

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  1. ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
  2. Branum G. D., Bowers B. A., Watters C. R., Cucchiaro G., Meyers W. C. Biliary response to glucagon and insulin following hepatic transplantation in humans. J Surg Res. 1990 Aug;49(2):121–125. doi: 10.1016/0022-4804(90)90249-2. [DOI] [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. Garberoglio C. A., Bickerstaff K. I., Baker A. L., Moossa A. R. Is glucagon a choleretic hormone at physiologic blood levels? Am J Surg. 1982 Jan;143(1):61–66. doi: 10.1016/0002-9610(82)90130-1. [DOI] [PubMed] [Google Scholar]
  5. Garberoglio C. A., Richter H. M., 3rd, Henarejos A., Moossa A. R., Baker A. L. Pharmacological and physiological doses of insulin and determinants of bile flow in dogs. Am J Physiol. 1983 Jul;245(1):G157–G163. doi: 10.1152/ajpgi.1983.245.1.G157. [DOI] [PubMed] [Google Scholar]
  6. Gorman C. K., Salter J. M., Penhos J. C. Effect of glucagon on lipids and glucose in normal and eviscerated rats and on isolated perfused rat livers. Metabolism. 1967 Dec;16(12):1140–1157. doi: 10.1016/0026-0495(67)90060-1. [DOI] [PubMed] [Google Scholar]
  7. Guettet C., Mathe D., Riottot M., Lutton C. Effects of chronic glucagon administration on cholesterol and bile acid metabolism. Biochim Biophys Acta. 1988 Nov 25;963(2):215–223. doi: 10.1016/0005-2760(88)90283-4. [DOI] [PubMed] [Google Scholar]
  8. Ishida T., Lewis R. M., Hartley C. J., Entman M. L., Field J. B. Comparison of hepatic extraction of insulin and glucagon in conscious and anesthetized dogs. Endocrinology. 1983 Mar;112(3):1098–1109. doi: 10.1210/endo-112-3-1098. [DOI] [PubMed] [Google Scholar]
  9. Jones R. S., Putnam W., Andersen D. K., Hanks J. B., Lebovitz H. E. Insulin's effect on bile flow and lipid excretion during euglycemia and hypoglycemia. Dig Dis Sci. 1984 Jan;29(1):33–39. doi: 10.1007/BF01296859. [DOI] [PubMed] [Google Scholar]
  10. Kaminski D. L., Deshpande Y. G. Effect of theophylline on glucagon and secretin stimulated bile flow. Dig Dis Sci. 1984 Mar;29(3):261–266. doi: 10.1007/BF01296261. [DOI] [PubMed] [Google Scholar]
  11. Kaminski D. L., Deshpande Y., Beinfeld M. C. Effect of stimulation of endogenous glucagon secretion by amino acid administration on canine hepatic bile flow. Dig Dis Sci. 1987 Apr;32(4):401–408. doi: 10.1007/BF01296294. [DOI] [PubMed] [Google Scholar]
  12. Khedis A., Dumont M., Duval M., Erlinger S. Influence of glucagon on canalicular bile production in the dog. Biomedicine. 1974 Apr 10;21(4):176–181. [PubMed] [Google Scholar]
  13. Kortz W. J., Meyers W. C., Schirmer B. D., Jones R. S. Effects of dibutyryl cyclic AMP and theophylline on biliary cholesterol secretion. J Surg Res. 1984 Jan;36(1):62–70. doi: 10.1016/0022-4804(84)90068-4. [DOI] [PubMed] [Google Scholar]
  14. Larsen J. A., Krarup N., Thomsen Oø The effect of glucagon, dibutyrylic cyclic AMP and theophylline on bile production in the cat. Acta Physiol Scand. 1979 May;106(1):23–27. doi: 10.1111/j.1748-1716.1979.tb06365.x. [DOI] [PubMed] [Google Scholar]
  15. Meyers W. C., Jones R. S. Effect of glucagon and insulin upon biliary lipid secretion. Am J Surg. 1979 Jan;137(1):7–12. doi: 10.1016/0002-9610(79)90003-5. [DOI] [PubMed] [Google Scholar]
  16. Meyers W. C., Jones R. S. Glucagon or insulin suppressed biliary lipid excretion in dog and man. Ann Surg. 1979 Dec;190(6):709–718. doi: 10.1097/00000658-197912000-00007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meyers W. C., Wellman C., Quarfordt S. H., Jones R. S. Insulin or glucagon choleresis in the isolated perfused guinea pig liver. Proc Soc Exp Biol Med. 1983 May;173(1):56–62. doi: 10.3181/00379727-173-41609. [DOI] [PubMed] [Google Scholar]
  18. Rossi S. S., Converse J. L., Hofmann A. F. High pressure liquid chromatographic analysis of conjugated bile acids in human bile: simultaneous resolution of sulfated and unsulfated lithocholyl amidates and the common conjugated bile acids. J Lipid Res. 1987 May;28(5):589–595. [PubMed] [Google Scholar]
  19. Russell T. R., Searle G. L., Jones R. S. The choleretic mechanisms of sodium taurocholate, secretin, and glucagon. Surgery. 1975 Apr;77(4):498–504. [PubMed] [Google Scholar]
  20. Ruwart M. J., Kaminski D. L., Hahn J. Secretin-, glucagon-, and insulin-induced effects on bile flow and metabolism of isolated perfused canine and porcine liver. J Surg Res. 1979 Jun;26(6):674–680. doi: 10.1016/0022-4804(79)90064-7. [DOI] [PubMed] [Google Scholar]
  21. Schirmer B. D., Kortz W. J., Miller R. J., Christian K. G., Hayes E., Jones R. S. Glucagon lowers canine biliary cholesterol output at physiologic doses. Dig Dis Sci. 1986 Mar;31(3):297–304. doi: 10.1007/BF01318122. [DOI] [PubMed] [Google Scholar]
  22. Sewell R. B., Grinpukel S. A., Zinsmeister A. R., LaRusso N. F. Pharmacologic perturbation of rat liver lysosomes: effects on release of lysosomal enzymes and of lipids into bile. Gastroenterology. 1988 Oct;95(4):1088–1098. doi: 10.1016/0016-5085(88)90187-4. [DOI] [PubMed] [Google Scholar]
  23. Snow J. R., Jones R. S. The effect of insulin on bile-salt-independent canalicular secretion. Surgery. 1978 Apr;83(4):458–463. [PubMed] [Google Scholar]
  24. Thomsen O. O., Larsen J. A., Orskov H. Insulin-induced choleresis in relation to insulin concentrations in plasma and bile in the cat. Scand J Gastroenterol. 1982 Mar;17(2):297–303. doi: 10.3109/00365528209182057. [DOI] [PubMed] [Google Scholar]
  25. UNGER R. H., EISENTRAUT A. M., McCALL M. S., MADISON L. L. Glucagon antibodies and an immunoassay for glucagon. J Clin Invest. 1961 Jul;40:1280–1289. doi: 10.1172/JCI104357. [DOI] [PMC free article] [PubMed] [Google Scholar]

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