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. 1998 May;94(1):122–128. doi: 10.1046/j.1365-2567.1998.00462.x

Immunohistochemical investigation of the tissue distribution of mannan-binding lectin in non-infected and virus-infected chickens.

O L Nielsen 1, P H Jørgensen 1, J Hedemand 1, J C Jensenius 1, C Koch 1, S B Laursen 1
PMCID: PMC1364340  PMID: 9708196

Abstract

This paper describes the results of immuno-histochemical staining for chicken mannan-binding lectin (MBL) in formalin-fixed tissue sections from non-infected chickens, and from chickens infected with infectious laryngotracheitis virus (ILTV) or infectious bursal disease virus (IBDV). In the non-infected chickens, MBL was detected in the cytoplasm of a few hepatocytes and in the germinal centres of the caecal tonsils, whereas sections of kidney, heart muscle, spleen, cerebrum, thymus, adrenal gland, bursa of Fabricius, bone marrow and trachea were without staining. In the ILTV-infected chickens, an intense staining reaction for MBL was detected in the cytoplasm of all hepatocytes and on the surface of, and inside, ILTV-infected cells. Also in the IBDV-infected chickens, an intense staining reaction for MBL was detected in the cytoplasm of all hepatocytes. No staining was seen in the follicles of the bursa of Fabricius, but MBL was present in non-identified cells in the interstitium, and in the cytoplasm of macrophage-like cells, located peripheral to the ellipsoid of the spleen. These findings indicate the liver as the primary site of MBL synthesis, and points to up-regulation as a result of the viral infections. The location outside the liver could indicate a role of MBL in the immune defence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ezekowitz R. A., Kuhlman M., Groopman J. E., Byrn R. A. A human serum mannose-binding protein inhibits in vitro infection by the human immunodeficiency virus. J Exp Med. 1989 Jan 1;169(1):185–196. doi: 10.1084/jem.169.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fischer P. B., Ellermann-Eriksen S., Thiel S., Jensenius J. C., Mogensen S. C. Mannan-binding protein and bovine conglutinin mediate enhancement of herpes simplex virus type 2 infection in mice. Scand J Immunol. 1994 May;39(5):439–445. doi: 10.1111/j.1365-3083.1994.tb03398.x. [DOI] [PubMed] [Google Scholar]
  3. Gallego M., del Cacho E., Bascuas J. A. Antigen-binding cells in the cecal tonsil and Peyer's patches of the chicken after bovine serum albumin administration. Poult Sci. 1995 Mar;74(3):472–479. doi: 10.3382/ps.0740472. [DOI] [PubMed] [Google Scholar]
  4. Hartshorn K. L., Sastry K., White M. R., Anders E. M., Super M., Ezekowitz R. A., Tauber A. I. Human mannose-binding protein functions as an opsonin for influenza A viruses. J Clin Invest. 1993 Apr;91(4):1414–1420. doi: 10.1172/JCI116345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holmskov U., Holt P., Reid K. B., Willis A. C., Teisner B., Jensenius J. C. Purification and characterization of bovine mannan-binding protein. Glycobiology. 1993 Apr;3(2):147–153. doi: 10.1093/glycob/3.2.147. [DOI] [PubMed] [Google Scholar]
  6. Holmskov U., Malhotra R., Sim R. B., Jensenius J. C. Collectins: collagenous C-type lectins of the innate immune defense system. Immunol Today. 1994 Feb;15(2):67–74. doi: 10.1016/0167-5699(94)90136-8. [DOI] [PubMed] [Google Scholar]
  7. Holmskov U., Teisner B., Pedersen N. T., Laursen S. B., Rasmussen H. B., Jensenius J. C. Tissue localization of conglutinin, a bovine C-type lectin. Immunology. 1992 May;76(1):169–173. [PMC free article] [PubMed] [Google Scholar]
  8. Jeurissen S. H., Claassen E., Janse E. M. Histological and functional differentiation of non-lymphoid cells in the chicken spleen. Immunology. 1992 Sep;77(1):75–80. [PMC free article] [PubMed] [Google Scholar]
  9. Jeurissen S. H., Scholten R., Hilgers L. A., Pol J. M., De Boer G. F. In situ detection by monoclonal antibody D-35.1 of cells infected with Marek's disease virus that interact with splenic ellipsoid-associated reticulum cells. Avian Dis. 1989 Oct-Dec;33(4):657–663. [PubMed] [Google Scholar]
  10. Kawasaki N., Kawasaki T., Yamashina I. Isolation and characterization of a mannan-binding protein from human serum. J Biochem. 1983 Sep;94(3):937–947. doi: 10.1093/oxfordjournals.jbchem.a134437. [DOI] [PubMed] [Google Scholar]
  11. Käufer I., Weiss E. Electron-microscope studies on the pathogenesis of infectious bursal disease after intrabursal application of the causal virus. Avian Dis. 1976 Jul-Sep;20(3):483–495. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Laursen S. B., Hedemand J. E., Thiel S., Willis A. C., Skriver E., Madsen P. S., Jensenius J. C. Collectin in a non-mammalian species: isolation and characterization of mannan-binding protein (MBP) from chicken serum. Glycobiology. 1995 Sep;5(6):553–561. doi: 10.1093/glycob/5.6.553. [DOI] [PubMed] [Google Scholar]
  14. Müller H. Replication of infectious bursal disease virus in lymphoid cells. Arch Virol. 1986;87(3-4):191–203. doi: 10.1007/BF01315299. [DOI] [PubMed] [Google Scholar]
  15. Nunoya T., Otaki Y., Tajima M., Hiraga M., Saito T. Occurrence of acute infectious bursal disease with high mortality in Japan and pathogenicity of field isolates in specific-pathogen-free chickens. Avian Dis. 1992 Jul-Sep;36(3):597–609. [PubMed] [Google Scholar]
  16. Oka S., Kawasaki T., Yamashina I. Isolation and characterization of mannan-binding proteins from chicken liver. Arch Biochem Biophys. 1985 Aug 15;241(1):95–105. doi: 10.1016/0003-9861(85)90366-2. [DOI] [PubMed] [Google Scholar]
  17. Olah I., Mandi Y., Beladi I., Glick B. Effect of human adenovirus on the ellipsoid-associated cells of the chicken's spleen. Poult Sci. 1990 Jun;69(6):929–933. doi: 10.3382/ps.0690929. [DOI] [PubMed] [Google Scholar]
  18. Oláh I., Glick B. Anti-vimentin monoclonal antibodies differentiate two resident cell populations in chicken spleen. Dev Comp Immunol. 1994 Jan-Feb;18(1):67–73. doi: 10.1016/0145-305x(94)90253-4. [DOI] [PubMed] [Google Scholar]
  19. Oláh I., Glick B. Splenic white pulp and associated vascular channels in chicken spleen. Am J Anat. 1982 Dec;165(4):445–480. doi: 10.1002/aja.1001650408. [DOI] [PubMed] [Google Scholar]
  20. Oláh I., Glick B., Taylor R. L., Jr Effect of soluble antigen on the ellipsoid-associated cells of the chicken's spleen. J Leukoc Biol. 1984 May;35(5):501–510. doi: 10.1002/jlb.35.5.501. [DOI] [PubMed] [Google Scholar]
  21. Schapira A. H., Keir G. Two-dimensional protein mapping by gold stain and immunoblotting. Anal Biochem. 1988 Feb 15;169(1):167–171. doi: 10.1016/0003-2697(88)90268-0. [DOI] [PubMed] [Google Scholar]
  22. Sugii S., Hirota Y. Identification and carbohydrate specificity of a chicken serum mannan-binding protein reactive with a Ra chemotype strain of Salmonella typhimurium. J Vet Med Sci. 1994 Aug;56(4):747–751. doi: 10.1292/jvms.56.747. [DOI] [PubMed] [Google Scholar]
  23. Tanimura N., Tsukamoto K., Nakamura K., Narita M., Maeda M. Association between pathogenicity of infectious bursal disease virus and viral antigen distribution detected by immunohistochemistry. Avian Dis. 1995 Jan-Mar;39(1):9–20. [PubMed] [Google Scholar]
  24. Thiel S., Holmskov U., Hviid L., Laursen S. B., Jensenius J. C. The concentration of the C-type lectin, mannan-binding protein, in human plasma increases during an acute phase response. Clin Exp Immunol. 1992 Oct;90(1):31–35. doi: 10.1111/j.1365-2249.1992.tb05827.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thiel S., Vorup-Jensen T., Stover C. M., Schwaeble W., Laursen S. B., Poulsen K., Willis A. C., Eggleton P., Hansen S., Holmskov U. A second serine protease associated with mannan-binding lectin that activates complement. Nature. 1997 Apr 3;386(6624):506–510. doi: 10.1038/386506a0. [DOI] [PubMed] [Google Scholar]
  26. Turner M. W. Mannose-binding lectin: the pluripotent molecule of the innate immune system. Immunol Today. 1996 Nov;17(11):532–540. doi: 10.1016/0167-5699(96)10062-1. [DOI] [PubMed] [Google Scholar]

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