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. 1998 Dec;95(4):612–617. doi: 10.1046/j.1365-2567.1998.00642.x

Cross-talk between gammadelta T lymphocytes and immune cells in humoral response.

M Szczepanik 1, B Nowak 1, P W Askenase 1, W Ptak 1
PMCID: PMC1364360  PMID: 9893053

Abstract

The role of gamma delta T cells in immunoregulation is largely unknown. In the current study we noted that gamma delta T cells play a positive role in the humoral response. These positively acting gamma delta T cells are required for the successful adoptive cell transfer of the humoral response, as well as for in vitro generation of plaque-forming cells (PFC). The presented results show that gammadelta T cells cause an increase in interleukin-10 (IL-10) production, which partly elucidates the mechanism of action of these cells. However, experiments with cell culture inserts strongly suggest that direct cell-cell contact between immune and gamma delta H-2-compatible regulatory T cells is critical to the exertion of the positive immunoregulatory function of gamma delta cells. The mechanism of cross-talk between these two cell populations is still not clear but we regard as most likely that the positively acting gamma delta T cells may interact with a complex of heat-shock protein-non-polymorphic MHC (IB) on the surface of T helper type 2 and/or B cells. This could provide, by direct cell-cell contact, the cognate recognition between gamma delta T-cell receptors and heat-shock protein-MHC that leads to positive internal signalling in the immune cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askenase P. W., Szczepanik M., Ptak M., Paliwal V., Ptak W. Gamma delta T cells in normal spleen assist immunized alpha beta T cells in the adoptive cell transfer of contact sensitivity. Effect of Bordetella pertussis, cyclophosphamide, and antibodies to determinants on suppressor cells. J Immunol. 1995 Apr 15;154(8):3644–3653. [PubMed] [Google Scholar]
  2. Bonneville M., Ito K., Krecko E. G., Itohara S., Kappes D., Ishida I., Kanagawa O., Janeway C. A., Murphy D. B., Tonegawa S. Recognition of a self major histocompatibility complex TL region product by gamma delta T-cell receptors. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5928–5932. doi: 10.1073/pnas.86.15.5928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Born W. K., O'Brien R. L., Modlin R. L. Antigen specificity of gamma delta T lymphocytes. FASEB J. 1991 Sep;5(12):2699–2705. doi: 10.1096/fasebj.5.12.1717333. [DOI] [PubMed] [Google Scholar]
  4. Born W., Hall L., Dallas A., Boymel J., Shinnick T., Young D., Brennan P., O'Brien R. Recognition of a peptide antigen by heat shock--reactive gamma delta T lymphocytes. Science. 1990 Jul 6;249(4964):67–69. doi: 10.1126/science.1695022. [DOI] [PubMed] [Google Scholar]
  5. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  6. Ferrick D. A., Schrenzel M. D., Mulvania T., Hsieh B., Ferlin W. G., Lepper H. Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo. Nature. 1995 Jan 19;373(6511):255–257. doi: 10.1038/373255a0. [DOI] [PubMed] [Google Scholar]
  7. Fiorentino D. F., Bond M. W., Mosmann T. R. Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J Exp Med. 1989 Dec 1;170(6):2081–2095. doi: 10.1084/jem.170.6.2081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fujihashi K., Kiyono H., Aicher W. K., Green D. R., Singh B., Eldridge J. H., McGhee J. R. Immunoregulatory function of CD3+, CD4-, and CD8- T cells. Gamma delta T cell receptor-positive T cells from nude mice abrogate oral tolerance. J Immunol. 1989 Dec 1;143(11):3415–3422. [PubMed] [Google Scholar]
  9. Fujihashi K., Taguchi T., Aicher W. K., McGhee J. R., Bluestone J. A., Eldridge J. H., Kiyono H. Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help. J Exp Med. 1992 Mar 1;175(3):695–707. doi: 10.1084/jem.175.3.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fujihashi K., Taguchi T., McGhee J. R., Eldridge J. H., Bruce M. G., Green D. R., Singh B., Kiyono H. Regulatory function for murine intraepithelial lymphocytes. Two subsets of CD3+, T cell receptor-1+ intraepithelial lymphocyte T cells abrogate oral tolerance. J Immunol. 1990 Oct 1;145(7):2010–2019. [PubMed] [Google Scholar]
  11. Go N. F., Castle B. E., Barrett R., Kastelein R., Dang W., Mosmann T. R., Moore K. W., Howard M. Interleukin 10, a novel B cell stimulatory factor: unresponsiveness of X chromosome-linked immunodeficiency B cells. J Exp Med. 1990 Dec 1;172(6):1625–1631. doi: 10.1084/jem.172.6.1625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goodman T., Lefrancois L. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989 Nov 1;170(5):1569–1581. doi: 10.1084/jem.170.5.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hiromatsu K., Yoshikai Y., Matsuzaki G., Ohga S., Muramori K., Matsumoto K., Bluestone J. A., Nomoto K. A protective role of gamma/delta T cells in primary infection with Listeria monocytogenes in mice. J Exp Med. 1992 Jan 1;175(1):49–56. doi: 10.1084/jem.175.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaufmann S. H., Blum C., Yamamoto S. Crosstalk between alpha/beta T cells and gamma/delta T cells in vivo: activation of alpha/beta T-cell responses after gamma/delta T-cell modulation with the monoclonal antibody GL3. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9620–9624. doi: 10.1073/pnas.90.20.9620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kaufmann S. H. gamma/delta and other unconventional T lymphocytes: what do they see and what do they do? Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2272–2279. doi: 10.1073/pnas.93.6.2272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lefrancois L., Goodman T. In vivo modulation of cytolytic activity and Thy-1 expression in TCR-gamma delta+ intraepithelial lymphocytes. Science. 1989 Mar 31;243(4899):1716–1718. doi: 10.1126/science.2564701. [DOI] [PubMed] [Google Scholar]
  17. McMenamin C., McKersey M., Kühnlein P., Hünig T., Holt P. G. Gamma delta T cells down-regulate primary IgE responses in rats to inhaled soluble protein antigens. J Immunol. 1995 May 1;154(9):4390–4394. [PubMed] [Google Scholar]
  18. McMenamin C., Pimm C., McKersey M., Holt P. G. Regulation of IgE responses to inhaled antigen in mice by antigen-specific gamma delta T cells. Science. 1994 Sep 23;265(5180):1869–1871. doi: 10.1126/science.7916481. [DOI] [PubMed] [Google Scholar]
  19. Ptak W., Askenase P. W. Gamma delta T cells assist alpha beta T cells in adoptive transfer of contact sensitivity. J Immunol. 1992 Dec 1;149(11):3503–3508. [PubMed] [Google Scholar]
  20. Ptak W., Szczepanik M., Ramabhadran R., Askenase P. W. Immune or normal gamma delta T cells that assist alpha beta T cells in elicitation of contact sensitivity preferentially use V gamma 5 and V delta 4 variable region gene segments. J Immunol. 1996 Feb 1;156(3):976–986. [PubMed] [Google Scholar]
  21. Szczepanik M., Anderson L. R., Ushio H., Ptak W., Owen M. J., Hayday A. C., Askenase P. W. Gamma delta T cells from tolerized alpha beta T cell receptor (TCR)-deficient mice inhibit contact sensitivity-effector T cells in vivo, and their interferon-gamma production in vitro. J Exp Med. 1996 Dec 1;184(6):2129–2139. doi: 10.1084/jem.184.6.2129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Szczepanik M., Anderson L. R., Ushio H., Ptak W., Owen M. J., Hayday A. C., Askenase P. W. Gamma/delta T cells from tolerized alpha/beta-TCR-deficient mice antigen specifically inhibit contact sensitivity in vivo and IFN-gamma production in vitro. Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):373–375. doi: 10.1159/000237607. [DOI] [PubMed] [Google Scholar]
  23. Szczepanik M., Lewis J., Geba G. P., Ptak W., Askenase P. W. Positive regulatory gamma delta T cells in contact sensitivity: augmented responses by in vivo treatment with anti-gamma delta monoclonal antibody, or anti-V gamma 5 or V delta 4. Immunol Invest. 1998 Jan-Feb;27(1-2):1–15. doi: 10.3109/08820139809070886. [DOI] [PubMed] [Google Scholar]
  24. Vidović D., Roglić M., McKune K., Guerder S., MacKay C., Dembić Z. Qa-1 restricted recognition of foreign antigen by a gamma delta T-cell hybridoma. Nature. 1989 Aug 24;340(6235):646–650. doi: 10.1038/340646a0. [DOI] [PubMed] [Google Scholar]

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