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. 1998 Sep;95(1):90–96. doi: 10.1046/j.1365-2567.1998.00560.x

Lack of SC/pIgR-mediated epithelial transport of a human polymeric IgA devoid of J chain: in vitro and in vivo studies.

J P Vaerman 1, A Langendries 1, D Giffroy 1, P Brandtzaeg 1, K Kobayashi 1
PMCID: PMC1364381  PMID: 9767462

Abstract

Three human polymeric IgA (pIgA) myeloma proteins of tetrameric size were compared for their J-chain content, their in vitro secretory component (SC)-binding ability, and their capacity to be transcytosed by polymeric immunoglobulin receptor (pIgR)-expressing epithelial cells in vitro and rat hepatocytes in vivo. One of the three pIgA preparations, pIgA-L, was shown to lack J chain and was unable to combine with purified free human and rat SC, whereas pIgA-G and pIgA-C contained J chain and combined readily with SC. Furthermore, pIgA-L was not transferred into rat bile after intravenous injection, and was hardly transported apically by polarized Madin-Darbey canine kidney cell monolayers expressing the human pIgR, whereas pIgA-G and pIgA-C were efficiently transported in both test systems. Together with our recent demonstration that antibodies to human J chain block the SC/pIgR-mediated epithelial transport of pIgA, these data unanimously confirm the proposed key role of J chain in the epithelial transport of polymeric immunoglobulins into exocrine secretions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altamirano G. A., Barranco-Acosta C., van Roost E., Vaerman J. P. Isolation and characterization of secretory IgA (sIgA) and free secretory component (FSC) from rat bile. Mol Immunol. 1980 Dec;17(12):1525–1537. doi: 10.1016/0161-5890(80)90178-9. [DOI] [PubMed] [Google Scholar]
  2. Brandtzaeg P. Blocking effect of J chain and J-chain antibody on the binding of secretory component to human IgA and IgM. Scand J Immunol. 1975;4(8):837–842. doi: 10.1111/j.1365-3083.1975.tb03725.x. [DOI] [PubMed] [Google Scholar]
  3. Brandtzaeg P. Blocking effect of J chain and J-chain antibody on the binding of secretory component to human IgA and IgM. Scand J Immunol. 1975;4(8):837–842. doi: 10.1111/j.1365-3083.1975.tb03725.x. [DOI] [PubMed] [Google Scholar]
  4. Brandtzaeg P. Complex formation between secretory component and human immunoglobulins related to their content of J chain. Scand J Immunol. 1976;5(4):411–419. doi: 10.1111/j.1365-3083.1976.tb00295.x. [DOI] [PubMed] [Google Scholar]
  5. Brandtzaeg P. Human secretory component--VI. Immunoglobulin-binding properities. Immunochemistry. 1977 Mar;14(3):179–188. doi: 10.1016/0019-2791(77)90192-6. [DOI] [PubMed] [Google Scholar]
  6. Brandtzaeg P., Prydz H. Direct evidence for an integrated function of J chain and secretory component in epithelial transport of immunoglobulins. Nature. 1984 Sep 6;311(5981):71–73. doi: 10.1038/311071a0. [DOI] [PubMed] [Google Scholar]
  7. Brandtzaeg P. Role of J chain and secretory component in receptor-mediated glandular and hepatic transport of immunoglobulins in man. Scand J Immunol. 1985 Aug;22(2):111–146. doi: 10.1111/j.1365-3083.1985.tb01866.x. [DOI] [PubMed] [Google Scholar]
  8. Delacroix D. L., Dehennin J. P., Vaerman J. P. Influence of molecular size of IgA on its immunoassay by various techniques. II. Solid-phase radioimmunoassays. J Immunol Methods. 1982;48(3):327–337. doi: 10.1016/0022-1759(82)90333-7. [DOI] [PubMed] [Google Scholar]
  9. Delacroix D. L., Hodgson H. J., McPherson A., Dive C., Vaerman J. P. Selective transport of polymeric immunoglobulin A in bile. Quantitative relationships of monomeric and polymeric immunoglobulin A, immunoglobulin M, and other proteins in serum, bile, and saliva. J Clin Invest. 1982 Aug;70(2):230–241. doi: 10.1172/JCI110610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hendrickson B. A., Conner D. A., Ladd D. J., Kendall D., Casanova J. E., Corthesy B., Max E. E., Neutra M. R., Seidman C. E., Seidman J. G. Altered hepatic transport of immunoglobulin A in mice lacking the J chain. J Exp Med. 1995 Dec 1;182(6):1905–1911. doi: 10.1084/jem.182.6.1905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hendrickson B. A., Rindisbacher L., Corthesy B., Kendall D., Waltz D. A., Neutra M. R., Seidman J. G. Lack of association of secretory component with IgA in J chain-deficient mice. J Immunol. 1996 Jul 15;157(2):750–754. [PubMed] [Google Scholar]
  12. Jerry L. M., Kunkel H. G., Adams L. Stabilization of dissociable IgA2 proteins by secretory component. J Immunol. 1972 Aug;109(2):275–283. [PubMed] [Google Scholar]
  13. Jerry L. M., Kunkel H. G., Grey H. M. Absence of disulfide bonds linking the heavy and light chains: a property of a genetic variant of gamma-A2 globulins. Proc Natl Acad Sci U S A. 1970 Mar;65(3):557–563. doi: 10.1073/pnas.65.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnsson B., Löfås S., Lindquist G. Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. Anal Biochem. 1991 Nov 1;198(2):268–277. doi: 10.1016/0003-2697(91)90424-r. [DOI] [PubMed] [Google Scholar]
  15. Kobayashi K., Vaerman J. P., Heremans J. F. A facile method to isolate J-chain from polymeric serum IgA or IgM. Immunochemistry. 1974 Mar;11(3):169–171. doi: 10.1016/0019-2791(74)90217-1. [DOI] [PubMed] [Google Scholar]
  16. Kobayashi K., Vaerman J. P., Heremans J. F. Improved procedure for the isolation of J-chain from human polymeric immunoglobulins. Biochim Biophys Acta. 1973 Mar 23;303(1):105–117. doi: 10.1016/0005-2795(73)90152-9. [DOI] [PubMed] [Google Scholar]
  17. Kobayashi K., Vaerman J. P., Heremans J. F. J-chain determinants in polymeric immunoglobulins. Eur J Immunol. 1973 Apr;3(4):185–191. doi: 10.1002/eji.1830030402. [DOI] [PubMed] [Google Scholar]
  18. Koshland M. E., Chapuis R. M., Recht B., Brown J. C. Selective proteolysis of the J chain component in human polymeric immunoglobulin. J Immunol. 1977 Mar;118(3):775–781. [PubMed] [Google Scholar]
  19. Lemaître-Coelho I., Altamirano G. A., Barranco-Acosta C., Meykens R., Vaerman J. P. In vivo experiments involving secretory component in the rat hepatic transfer of polymeric IgA from blood into bile. Immunology. 1981 Jun;43(2):261–270. [PMC free article] [PubMed] [Google Scholar]
  20. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  21. Mostov K. E. Transepithelial transport of immunoglobulins. Annu Rev Immunol. 1994;12:63–84. doi: 10.1146/annurev.iy.12.040194.000431. [DOI] [PubMed] [Google Scholar]
  22. Natvig I. B., Johansen F. E., Nordeng T. W., Haraldsen G., Brandtzaeg P. Mechanism for enhanced external transfer of dimeric IgA over pentameric IgM: studies of diffusion, binding to the human polymeric Ig receptor, and epithelial transcytosis. J Immunol. 1997 Nov 1;159(9):4330–4340. [PubMed] [Google Scholar]
  23. Schiff J. M., Fisher M. M., Underdown B. J. Secretory component as the mucosal transport receptor: separation of physicochemically analogous human IgA fractions with different receptor-binding capacities. Mol Immunol. 1986 Jan;23(1):45–56. doi: 10.1016/0161-5890(86)90170-7. [DOI] [PubMed] [Google Scholar]
  24. Socken D. J., Underdown B. J. Comparison of human, bovine and rabbit secretory component-immunoglobulin interactions. Immunochemistry. 1978 Jul;15(7):499–506. doi: 10.1016/0161-5890(78)90080-9. [DOI] [PubMed] [Google Scholar]
  25. Song W., Vaerman J. P., Mostov K. E. Dimeric and tetrameric IgA are transcytosed equally by the polymeric Ig receptor. J Immunol. 1995 Jul 15;155(2):715–721. [PubMed] [Google Scholar]
  26. Takahashi T., Iwase T., Takenouchi N., Saito M., Kobayashi K., Moldoveanu Z., Mestecky J., Moro I. The joining (J) chain is present in invertebrates that do not express immunoglobulins. Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1886–1891. doi: 10.1073/pnas.93.5.1886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tomasi T. B., Czerwinski D. S. Naturally occurring polymers of IgA lacking J chain. Scand J Immunol. 1976;5(6-7):647–653. doi: 10.1111/j.1365-3083.1976.tb03014.x. [DOI] [PubMed] [Google Scholar]
  28. Vaerman J. P., Langendries A. E., Giffroy D. A., Kaetzel C. S., Fiani C. M., Moro I., Brandtzaeg P., Kobayashi K. Antibody against the human J chain inhibits polymeric Ig receptor-mediated biliary and epithelial transport of human polymeric IgA. Eur J Immunol. 1998 Jan;28(1):171–182. doi: 10.1002/(SICI)1521-4141(199801)28:01<171::AID-IMMU171>3.0.CO;2-#. [DOI] [PubMed] [Google Scholar]
  29. Vaerman J. P., Langendries A., Vander Maelen C. Homogenous IgA monomers, dimers, trimers and tetramers from the same IgA myeloma serum. Immunol Invest. 1995 May;24(4):631–641. doi: 10.3109/08820139509066863. [DOI] [PubMed] [Google Scholar]

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