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. 1967 Jul;191(2):289–308. doi: 10.1113/jphysiol.1967.sp008251

The partition of sodium fluxes in isolated toad oocytes

D A T Dick, E J A Lea
PMCID: PMC1365454  PMID: 6050106

Abstract

1. The rate constant for Na efflux from the oocyte calculated from (d/dt) (ln [Na*]i]) is only approximately 52% of that calculated from (d/dt)[(ln(d[Na*]i)dt)]. The difference may be interpreted by supposing that 48% of the internal Na of the oocyte is either bound to proteins or sequestered in cell organelles.

2. The mean rate constant for Na efflux was 6·4 × 10-3 min-1 corresponding to an apparent Na efflux rate of 13·3 p-mole/cm2.sec. When this is corrected for the increase in surface area produced by microvilli the true efflux rate is 1·1-1·3 p-mole/cm2.sec.

3. The action of ouabain (1-5 μM) appears to involve two different effects: (a) there is 48-65% inhibition of the membrane Na pump, and (b) there is a release of some of the sequestered Na in the cell.

4. Removal of external K causes a 40% reduction in Na efflux although this value may be an underestimation owing to the presence of K which has leaked from the cell and may be retained near the cell surface.

5. Raising the external K concentration to 15 mM reduces the inhibitory effect of ouabain by approximately a half.

6. It was concluded that the Na pump in the toad oocyte may have a slightly lower level of activity than that in frog muscle, but that its general properties are similar to those in frog muscle and some other animal cells.

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Selected References

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