Abstract
Zone centrifugation of mixtures of two labeled DNA's at low concentrations in density gradients of sucrose permits accurate measurement of relative sedimentation rates. The individual rates are constant during the run. Measurements with DNA's from phages T2, T5, and lambda conform to the relation D2/D1 = (M2/M1)0.35, where D and M refer to distances sedimented and molecular weights of the DNA pair. The results show that high molecular weight DNA's sediment artificially fast in the optical centrifuge, owing to a hitherto unknown effect of molecular interactions. The molecular weight of lambda DNA is 31 million, measured either from sedimentation rate or from tests of fragility under shear.
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Selected References
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- BURGI E., HERSHEY A. D. A relative molecular weight series derived from the nucleic acid of bacteriophage T2. J Mol Biol. 1961 Aug;3:458–472. doi: 10.1016/s0022-2836(61)80058-2. [DOI] [PubMed] [Google Scholar]
- Doty P., McGill B. B., Rice S. A. THE PROPERTIES OF SONIC FRAGMENTS OF DEOXYRIBOSE NUCLEIC ACID. Proc Natl Acad Sci U S A. 1958 May;44(5):432–438. doi: 10.1073/pnas.44.5.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERSHEY A. D., GOLDBERG E., BURGI E., INGRAHAM L. Local denaturation of DNA by shearing forces and by heat. J Mol Biol. 1963 Mar;6:230–243. doi: 10.1016/s0022-2836(63)80072-8. [DOI] [PubMed] [Google Scholar]
- LEVINTHAL C., DAVISON P. F. Degradation of deoxyribonucleic acid under hydrodynamic shearing forces. J Mol Biol. 1961 Oct;3:674–683. doi: 10.1016/s0022-2836(61)80030-2. [DOI] [PubMed] [Google Scholar]
- RUBENSTEIN I., THOMAS C. A., Jr, HERSHEY A. D. The molecular weights of T2 bacteriophage DNA and its first and second breakage products. Proc Natl Acad Sci U S A. 1961 Aug;47:1113–1122. doi: 10.1073/pnas.47.8.1113. [DOI] [PMC free article] [PubMed] [Google Scholar]