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. 1969 Jan;9(1):90–112. doi: 10.1016/S0006-3495(69)86371-X

Regulation of Cell Division in Escherichia coli

Olga Pierucci
PMCID: PMC1367416  PMID: 4882571

Abstract

The rate of cell division was measured in cultures of Escherichia coli B/r strain after periods of partial or complete inhibition of deoxyribonucleic acid (DNA) synthesis. The rate of DNA synthesis was temporarily decreased by removing thymidine from the growth medium or replacing it with 5-bromouracil. After restoration of DNA synthesis, a temporary period of accelerated cell division was observed. The results were consistent with the idea that chromosome replication begins when an initiator complement of fixed size accumulated in the cell. The increase in the potential for the initiation of new replication points during inhibition of DNA synthesis results in an increase in the rate of cell division after an interval which encompasses the time for the arrival of these replication points to the termini of the chromosomes and the time from this event to division.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Tomizawa J. Replication of the escherichia coli K12 chromosome. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1911–1918. doi: 10.1073/pnas.58.5.1911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARNER H. D., COHEN S. S. Synchronization of division of a thymineless mutant of Escherichia coli. J Bacteriol. 1956 Jul;72(1):115–123. doi: 10.1128/jb.72.1.115-123.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyle J. V., Goss W. A., Cook T. M. Induction of excessive deoxyribonucleic acid synthesis in Escherichia coli by nalidixic acid. J Bacteriol. 1967 Nov;94(5):1664–1671. doi: 10.1128/jb.94.5.1664-1671.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark D. J. Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B-r. J Bacteriol. 1968 Oct;96(4):1214–1224. doi: 10.1128/jb.96.4.1214-1224.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donachie W. D., Hobbs D. G. Recovery from "thymineless death" in Escherichia coli 15T. Biochem Biophys Res Commun. 1967 Oct 26;29(2):172–177. doi: 10.1016/0006-291x(67)90582-7. [DOI] [PubMed] [Google Scholar]
  6. FORRO F., Jr, WERTHEIMER S. A. The organization and replication of deoxyribonucleic acid in thymine-deficient strains of Escherichia coli. Biochim Biophys Acta. 1960 May 6;40:9–21. doi: 10.1016/0006-3002(60)91310-x. [DOI] [PubMed] [Google Scholar]
  7. HANAWALT P. C., MAALOE O., CUMMINGS D. J., SCHAECHTER M. The normal DNA replication cycle. II. J Mol Biol. 1961 Apr;3:156–165. doi: 10.1016/s0022-2836(61)80042-9. [DOI] [PubMed] [Google Scholar]
  8. HELMSTETTER C. E., CUMMINGS D. J. AN IMPROVED METHOD FOR THE SELECTION OF BACTERIAL CELLS AT DIVISION. Biochim Biophys Acta. 1964 Mar 16;82:608–610. doi: 10.1016/0304-4165(64)90453-2. [DOI] [PubMed] [Google Scholar]
  9. Helmstetter C. E., Pierucci O. Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968 May;95(5):1627–1633. doi: 10.1128/jb.95.5.1627-1633.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kallenbach N. R., Ma R. Initiation of deoxyribonucleic acid synthesis after thymine starvation of Bacillus subtilis. J Bacteriol. 1968 Feb;95(2):304–309. doi: 10.1128/jb.95.2.304-309.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LARK C., LARK K. G. EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI. J Mol Biol. 1964 Oct;10:120–136. doi: 10.1016/s0022-2836(64)80032-2. [DOI] [PubMed] [Google Scholar]
  12. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  13. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  14. NAKADA D. Involvement of newly-formed protein in the syntheses of deoxyibonucleic acid. Biochim Biophys Acta. 1960 Nov 4;44:241–244. doi: 10.1016/0006-3002(60)91559-6. [DOI] [PubMed] [Google Scholar]
  15. PRITCHARD R. H., LARK K. G. INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI. J Mol Biol. 1964 Aug;9:288–307. doi: 10.1016/s0022-2836(64)80208-4. [DOI] [PubMed] [Google Scholar]
  16. Pato M. L., Glaser D. A. The origin and direction of replication of the chromosome of Escherichia coli B-r. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1268–1274. doi: 10.1073/pnas.60.4.1268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pritchard R. H. Replication of the bacterial chromosome. Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):258–266. doi: 10.1098/rspb.1966.0028. [DOI] [PubMed] [Google Scholar]
  18. Swenson P. A., Setlow R. B. Effects of ultraviolet radiation on macromolecular synthesis in Escherichia coli. J Mol Biol. 1966 Jan;15(1):201–219. doi: 10.1016/s0022-2836(66)80221-8. [DOI] [PubMed] [Google Scholar]
  19. Wolf B., Newman A., Glaser D. A. On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968 Mar 28;32(3):611–629. doi: 10.1016/0022-2836(68)90346-x. [DOI] [PubMed] [Google Scholar]

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